##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	V400_input.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27714554
Sequences flagged as poor quality	0
Sequence length	36
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.91323187087911	39.0	38.0	40.0	35.0	40.0
2	37.73345156483485	39.0	38.0	40.0	33.0	40.0
3	37.73032587859794	39.0	38.0	40.0	33.0	40.0
4	37.67241623300162	39.0	38.0	40.0	33.0	40.0
5	37.60688539314037	39.0	38.0	40.0	33.0	40.0
6	37.658951141699774	39.0	38.0	40.0	33.0	40.0
7	37.641199133134165	39.0	38.0	40.0	33.0	40.0
8	37.60271072736729	39.0	38.0	40.0	33.0	40.0
9	37.30030802588416	39.0	38.0	40.0	33.0	40.0
10	37.547724888518864	39.0	38.0	40.0	33.0	40.0
11	37.68243425457974	39.0	38.0	40.0	33.0	40.0
12	37.64358091420125	39.0	38.0	40.0	33.0	40.0
13	37.60888206968801	39.0	38.0	40.0	33.0	40.0
14	37.55420531032179	39.0	38.0	40.0	33.0	40.0
15	37.492870136030334	39.0	38.0	40.0	33.0	40.0
16	37.55664594133465	39.0	38.0	40.0	33.0	40.0
17	37.53690977671876	39.0	38.0	40.0	33.0	40.0
18	37.47500926769379	39.0	38.0	40.0	33.0	40.0
19	37.30862318765801	39.0	37.0	40.0	33.0	40.0
20	37.37888349204537	39.0	37.0	40.0	33.0	40.0
21	37.35432000818054	39.0	37.0	40.0	33.0	40.0
22	37.206754581004624	39.0	37.0	40.0	33.0	40.0
23	37.26187067632407	39.0	37.0	40.0	33.0	40.0
24	37.21220016746436	39.0	37.0	40.0	33.0	40.0
25	37.086956116991814	39.0	36.0	40.0	33.0	40.0
26	37.014661285907756	39.0	36.0	40.0	33.0	40.0
27	36.954569862462876	39.0	36.0	40.0	32.0	40.0
28	36.71949940092848	39.0	36.0	40.0	31.0	40.0
29	36.51149850724641	39.0	36.0	40.0	31.0	40.0
30	36.52214630623318	39.0	36.0	40.0	31.0	40.0
31	36.42551884471964	39.0	36.0	40.0	31.0	40.0
32	36.24384747450744	39.0	35.0	40.0	31.0	40.0
33	36.258572048462334	38.0	35.0	40.0	31.0	40.0
34	36.158068933745064	38.0	35.0	40.0	31.0	40.0
35	35.934851342006084	38.0	35.0	40.0	30.0	40.0
36	35.959384156064715	38.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-5.715656007104869
1	2	-7.006493557918837
1	3	-6.089469707308382
1	4	-6.085094827893528
1	5	-7.0217282060808905
1	6	-6.7536156598446695
1	7	-6.190016216152831
1	8	-6.145836832574787
1	9	-6.257116306346177
1	10	-7.380377729900012
1	11	-7.291586722629873
1	12	-7.1377190805158435
1	13	-6.425995089888666
1	14	-6.937708279717828
1	15	-7.949726376020543
1	16	-7.7224702773459235
1	17	-7.335784465400003
1	18	-7.455763704208845
1	19	-9.599014243142605
1	20	-8.254981497536846
1	21	-8.287568478751997
1	22	-9.434752819395356
1	23	-8.111439775339896
1	24	-7.918575701134248
1	25	-8.886747142966975
1	26	-9.227359450313855
1	27	-8.868032137034024
1	28	-9.233339455365211
1	29	-10.316048193061047
1	30	-9.504104523136778
1	31	-9.86661416959815
1	32	-11.16199377344256
1	33	-10.94141091791294
1	34	-11.331301454190005
1	35	-12.992866404636917
1	36	-12.986918316824212
2	1	-4.417412972448744
2	2	-5.56726822932395
2	3	-4.8017310593956495
2	4	-4.809160549500653
2	5	-5.403899772252807
2	6	-5.125417274913033
2	7	-4.801579653340163
2	8	-4.753461436992161
2	9	-4.594250421181165
2	10	-8.785549524842601
2	11	-5.3721970604347575
2	12	-5.159055101247198
2	13	-5.073752085472336
2	14	-5.309484848341796
2	15	-9.32426038725134
2	16	-6.209625757258149
2	17	-5.59333281292632
2	18	-5.787039712081711
2	19	-11.54061642014537
2	20	-6.358159470602384
2	21	-6.325664054931973
2	22	-7.8186081180002525
2	23	-6.303689938700565
2	24	-6.206353726527791
2	25	-10.022210673108827
2	26	-7.264058736523058
2	27	-6.824213711265507
2	28	-7.211956727288079
2	29	-10.845532788511665
2	30	-7.338981631212516
2	31	-7.2427120387439565
2	32	-11.183868128381576
2	33	-8.345374829996633
2	34	-8.652523155323472
2	35	-13.752072452071332
2	36	-10.189458723115667
3	1	-3.549869101158592
3	2	-3.8792222510644194
3	3	-3.4196022294379205
3	4	-3.642510136176476
3	5	-4.302963378653018
3	6	-4.020549857951863
3	7	-3.664227970955004
3	8	-3.5615389192562503
3	9	-3.3038845648089747
3	10	-4.900972867779146
3	11	-4.22917919476069
3	12	-3.936718149417146
3	13	-3.9154612387114014
3	14	-4.168230460274444
3	15	-4.880767675987379
3	16	-4.652428696773995
3	17	-4.248917421191948
3	18	-4.298130289244938
3	19	-5.848932293702543
3	20	-4.804991382488758
3	21	-4.874499296843368
3	22	-5.9606934841743175
3	23	-4.935604975207248
3	24	-4.969889443130626
3	25	-5.786150538288524
3	26	-5.24780950260844
3	27	-5.206970556972291
3	28	-5.285676768188864
3	29	-5.95485173123609
3	30	-5.322298255439527
3	31	-5.529965550940464
3	32	-6.879434973922141
3	33	-5.9627098005945065
3	34	-6.325604852062508
3	35	-7.740411747867956
3	36	-7.208749711431491
4	1	-2.8349810166587233
4	2	-3.175716238800966
4	3	-2.743675690687759
4	4	-2.9279976035903132
4	5	-3.5205963477170172
4	6	-3.175272792296184
4	7	-2.876864554791993
4	8	-2.9255181097667986
4	9	-2.6956149648781818
4	10	-3.819245734050398
4	11	-3.2754818535583894
4	12	-3.2260276299402832
4	13	-3.2367007677130033
4	14	-3.336566095894341
4	15	-4.034795144142706
4	16	-3.6717484912434273
4	17	-3.5693841422804553
4	18	-3.563930202806212
4	19	-4.88079568514916
4	20	-3.9015381671945306
4	21	-4.145530839084721
4	22	-5.054482219401979
4	23	-4.034660036473014
4	24	-4.046755634052431
4	25	-4.793221598958354
4	26	-4.392080834060124
4	27	-4.206321957533767
4	28	-4.366215592498534
4	29	-5.021930786973627
4	30	-4.423539044290784
4	31	-4.581622982714627
4	32	-5.5473388879008425
4	33	-5.300966036507017
4	34	-5.095332406999848
4	35	-6.301639874715249
4	36	-5.815927807829556
5	1	-2.557365810238373
5	2	-2.528179022180602
5	3	-2.4478507774628753
5	4	-2.561921794101906
5	5	-3.4721849117945283
5	6	-2.6407226846769376
5	7	-2.424600037866554
5	8	-2.3658645141969075
5	9	-2.1776703962175503
5	10	-3.2991516846486846
5	11	-2.7189372300111643
5	12	-2.5886942122775523
5	13	-2.6189255181959865
5	14	-2.699539667739863
5	15	-3.3569205743091075
5	16	-3.1047591673468915
5	17	-2.843801012861455
5	18	-2.8882158220790473
5	19	-3.9512318680513516
5	20	-3.4782271726173377
5	21	-3.228495237722882
5	22	-5.967171255634579
5	23	-3.174001316482048
5	24	-3.246440783558782
5	25	-4.018318944593439
5	26	-3.5708380636184955
5	27	-3.3927822582983183
5	28	-3.5405923570419517
5	29	-4.055818347369701
5	30	-3.5696436794500386
5	31	-3.4007029123877786
5	32	-4.53479842050416
5	33	-3.808977559844557
5	34	-3.808805760668996
5	35	-4.937917257448774
5	36	-4.427656187592568
6	1	-2.2596515998804634
6	2	-2.3931599718695153
6	3	-1.9212766538894428
6	4	-2.234604624138001
6	5	-2.5461568187982735
6	6	-2.138337952265779
6	7	-2.00500063674626
6	8	-1.9703591874986088
6	9	-1.673404094801306
6	10	-2.794524509717
6	11	-2.2756148172913484
6	12	-2.0479600339361355
6	13	-2.145648835768384
6	14	-2.24280616029683
6	15	-2.8005094989589168
6	16	-2.4033543834562607
6	17	-2.3310094799163394
6	18	-2.4275628486287957
6	19	-3.5157380675208927
6	20	-2.5712205699434136
6	21	-2.6397371022471816
6	22	-3.097971597640523
6	23	-2.818348965113465
6	24	-2.752737889507557
6	25	-3.285021080925084
6	26	-2.725288721635195
6	27	-2.6830414317618434
6	28	-2.6985654570162865
6	29	-3.020905370562822
6	30	-2.6558497761695463
6	31	-2.72197559260983
6	32	-3.4207046680168105
6	33	-2.9062448835108228
6	34	-2.956585474922683
6	35	-4.067752379896376
6	36	-3.6185202490455453
7	1	-1.7228203958840425
7	2	-1.7231072014170508
7	3	-1.4599114974783802
7	4	-1.5766598831254512
7	5	-1.6705198909002235
7	6	-1.5634023367029144
7	7	-1.5293267975685012
7	8	-1.5868623080441253
7	9	-1.2137027665029336
7	10	-1.8389007857389075
7	11	-1.7915710482943084
7	12	-1.6736868326900307
7	13	-1.7904286963597968
7	14	-1.7825512493978763
7	15	-1.908120167505352
7	16	-1.9176417341417178
7	17	-1.9692560850697518
7	18	-1.9535628405333867
7	19	-2.774647999356901
7	20	-2.0983075132816182
7	21	-2.1373074717161558
7	22	-2.828914488101212
7	23	-2.5192436596185175
7	24	-2.2489207582618747
7	25	-2.3130339385240717
7	26	-2.184988308135239
7	27	-2.2056700864048295
7	28	-2.116774595790737
7	29	-2.2045392741249685
7	30	-2.2621129781473073
7	31	-2.0871823327399426
7	32	-2.363550250182371
7	33	-2.2897445686461353
7	34	-2.4490675417975822
7	35	-3.4062599395930775
7	36	-2.8768885681962075
8	1	-1.183094853974005
8	2	-1.2915931596006374
8	3	-1.1668725797017458
8	4	-1.458750100851205
8	5	-1.79590969032364
8	6	-1.1617552723017397
8	7	-1.3305660710597849
8	8	-1.3785882602663406
8	9	-0.874752035848978
8	10	-1.6109190417384553
8	11	-1.4401390329576316
8	12	-1.289780198551206
8	13	-1.5257835367006365
8	14	-1.6273038371957327
8	15	-1.4975513714834179
8	16	-1.5081933143530293
8	17	-1.4339578074850792
8	18	-1.5448091158981185
8	19	-2.2693800869302905
8	20	-1.5555498708569218
8	21	-1.6785912377141585
8	22	-1.9331576811664846
8	23	-1.6277212925281574
8	24	-1.5901957824672976
8	25	-1.7518403926781474
8	26	-1.7319632852726485
8	27	-1.614446656709326
8	28	-1.548275960952985
8	29	-1.6744888522495245
8	30	-1.5205045429926187
8	31	-1.5149575351877331
8	32	-1.713533865474993
8	33	-1.6513910683999455
8	34	-1.7248440389519786
8	35	-1.9762009394380655
8	36	-1.9292144735816166
9	1	-1.0241447143130884
9	2	-0.9204507282671983
9	3	-0.8744386712698358
9	4	-0.9157991347529517
9	5	-0.9145995452585325
9	6	-1.0533522266100732
9	7	-0.8802888248253424
9	8	-0.8668716896567119
9	9	-0.5674485416035466
9	10	-1.313384209282134
9	11	-1.0264168878326103
9	12	-0.9887363298498286
9	13	-1.009589221359711
9	14	-1.0178542314648737
9	15	-1.3892640627989579
9	16	-1.1998969518251172
9	17	-1.221589626642448
9	18	-1.241786808860624
9	19	-1.727233036918598
9	20	-1.304101115236783
9	21	-1.3770600385927523
9	22	-1.365146332632925
9	23	-1.2376945095947107
9	24	-1.5156608303600123
9	25	-1.8392775189570756
9	26	-1.2702353817976686
9	27	-1.3464491084442898
9	28	-1.3231728489438908
9	29	-1.3141029920668643
9	30	-1.2151730462572345
9	31	-1.1747697077970685
9	32	-1.784585544641537
9	33	-1.277156776733321
9	34	-1.4358342591583835
9	35	-2.106814364888443
9	36	-1.581796259340564
10	1	-0.7704933981522757
10	2	-0.5136405258184595
10	3	-0.44887278849645895
10	4	-0.5323466173054285
10	5	-0.6635656326551427
10	6	-1.907328944081641
10	7	-1.8320661322314962
10	8	-1.7319420177317326
10	9	-35.07001584972517
10	10	-2.035672976726353
10	11	-2.1771775195604732
10	12	-2.0415481230443646
10	13	-2.120809745201761
10	14	-1.9877248805731895
10	15	-2.10474936593063
10	16	-0.6281517485961885
10	17	-0.6064266506069131
10	18	-0.8159462096367989
10	19	-0.7525645371633232
10	20	-1.611431604998323
10	21	-0.9158225424594235
10	22	-2.395308075042756
10	23	-1.8189961539820843
10	24	-1.5358061686645925
10	25	-4.047980484705441
10	26	-5.25484538327326
10	27	-5.488286906368064
10	28	-27.001793692605368
10	29	-7.62226827866629
10	30	-7.764576427904046
10	31	-27.899308958503724
10	32	-9.132874342311055
10	33	-6.931789816008962
10	34	-7.114582132700686
10	35	-9.335318077301263
10	36	-7.61109713410389
11	1	-0.6419952193005756
11	2	-0.6770094382151299
11	3	-0.585081229219071
11	4	-0.7270350977250857
11	5	-0.7732102936677165
11	6	-1.397110803824198
11	7	-1.3932205572353809
11	8	-1.5154850452991866
11	9	-20.764169527103242
11	10	-1.6806026695428429
11	11	-1.5007941730432819
11	12	-1.5068540929337573
11	13	-1.6022444838123064
11	14	-1.573789734918897
11	15	-1.49109898998951
11	16	-0.6316826222889986
11	17	-0.6647675793770063
11	18	-0.7543173915638235
11	19	-0.8722241497958905
11	20	-0.7805308223943968
11	21	-0.9110180138551627
11	22	-2.3777563136094813
11	23	-0.7547116911965688
11	24	-0.8258972130826407
11	25	-1.115121555910406
11	26	-0.7789543677674544
11	27	-0.7849192610396969
11	28	-0.813129300541533
11	29	-0.6149816239092729
11	30	-0.6061135292800941
11	31	-0.6584099255713056
11	32	-0.74602202562793
11	33	-0.5793274512584645
11	34	-0.6055690749664393
11	35	-0.9020840980798965
11	36	-0.676025389722227
12	1	-0.3493861511077796
12	2	-0.3497235373309735
12	3	-0.4056853053721028
12	4	-0.3984554090700243
12	5	-0.38116941991997066
12	6	-0.49948667847309736
12	7	-0.4089445997399892
12	8	-0.4680136803840824
12	9	-0.011301884521770944
12	10	-0.4449938448734798
12	11	-0.39877686044347627
12	12	-0.5005759008463144
12	13	-0.447766119476924
12	14	-0.6646903952823919
12	15	-0.8504849432940986
12	16	-0.47757135908326376
12	17	-0.47101771072608045
12	18	-0.5990491013249226
12	19	-0.6618774083845906
12	20	-0.5753001899264518
12	21	-0.6533467804883486
12	22	-0.6829566440480974
12	23	-0.6782413474862281
12	24	-0.6025820928231767
12	25	-0.6070191400938398
12	26	-0.4604226924099706
12	27	-0.47281238757152977
12	28	-0.5094620456277354
12	29	-0.21591555396637574
12	30	-0.3980017378897571
12	31	-0.3429600978466283
12	32	-0.2367249144615755
12	33	-0.33603903457890283
12	34	-0.2798406071747621
12	35	-0.2805534393877025
12	36	-0.29991480252607516
13	1	-0.43205244549571376
13	2	-0.27362681087485186
13	3	-0.30058651951925697
13	4	-0.36664626330875194
13	5	-0.4101548402960802
13	6	-0.287809557410597
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119	6	-3.311847876189937
119	7	-2.828464589992066
119	8	-2.78300784839913
119	9	-2.383038909512038
119	10	-3.8623954289469324
119	11	-3.36229982145867
119	12	-3.074377920100808
119	13	-2.964846680776702
119	14	-3.193981567485281
119	15	-3.6872560643586283
119	16	-3.8379900098670205
119	17	-3.5467584147166917
119	18	-3.580647975018721
119	19	-4.549343581282706
119	20	-3.7496129841242762
119	21	-3.7950858587498004
119	22	-6.456340540306972
119	23	-3.9615359505719496
119	24	-3.9043119444264462
119	25	-4.442700165081021
119	26	-4.271917456632153
119	27	-4.041173693119447
119	28	-4.100256515791543
119	29	-4.861918905726867
119	30	-4.140892817484534
119	31	-4.130526745466824
119	32	-4.852039014909543
119	33	-4.866024079451865
119	34	-4.697098797930856
119	35	-5.608262043410086
119	36	-5.582633683774681
120	1	-3.5984854044362606
120	2	-4.573390872850354
120	3	-4.03052388018164
120	4	-4.01431893437433
120	5	-4.684430257912048
120	6	-4.511947017604001
120	7	-4.172446843871711
120	8	-4.033449555534773
120	9	-3.9086371361399443
120	10	-5.259331808340832
120	11	-4.711612951667391
120	12	-4.415187593158862
120	13	-4.4391714441117145
120	14	-4.5867539128432
120	15	-5.522383114769319
120	16	-5.169125992747368
120	17	-5.213834685808209
120	18	-5.089224955958876
120	19	-6.558351168069478
120	20	-5.189987648157242
120	21	-5.491721332863758
120	22	-6.5857270407887185
120	23	-5.692786372677087
120	24	-5.541038932409428
120	25	-6.266639421501836
120	26	-6.25335240673401
120	27	-5.837069591283022
120	28	-5.9140151477709715
120	29	-7.02773937660109
120	30	-6.172341540652219
120	31	-6.027048361252536
120	32	-7.568480834091368
120	33	-7.14704979878978
120	34	-6.998902830769971
120	35	-8.375559783698204
120	36	-8.474677199334192
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62430.0
3	5418.0
4	1778.0
5	1047.0
6	891.0
7	905.0
8	1559.0
9	2428.0
10	2753.0
11	2871.0
12	3483.0
13	3984.0
14	4641.0
15	5244.0
16	6241.0
17	7508.0
18	9571.0
19	11991.0
20	15630.0
21	21561.0
22	29652.0
23	40848.0
24	56460.0
25	79889.0
26	116583.0
27	167409.0
28	231538.0
29	310193.0
30	425828.0
31	533632.0
32	674512.0
33	831513.0
34	1092782.0
35	1468840.0
36	2088259.0
37	3421640.0
38	5484293.0
39	1.0452541E7
40	36208.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.52641187260449	26.22161431175465	26.344022606906055	25.907951208734808
2	23.016051421935206	27.965266913550185	25.84459053535554	23.17409112915907
3	23.691237038911993	27.733572007567957	25.89804037315882	22.67715058036123
4	23.981363726798563	26.35607269739935	26.257730865883676	23.404832709918406
5	24.24114420170716	27.26347679995139	26.98861760503164	21.506761393309812
6	24.186295763590497	26.83011604660858	25.33104086755284	23.652547322248086
7	23.7593937106114	26.633118469090284	26.970688397150468	22.636799423147853
8	23.22749989049075	26.524016947918412	26.84229015556231	23.40619300602853
9	23.12965117730635	25.967660437130103	27.199854088681334	23.702834296882212
10	23.26593708842694	26.76276083274811	26.026768393253057	23.944533685571887
11	23.624107391372778	26.361033989578182	26.161521487951784	23.85333713109726
12	23.573722312110814	26.246888187340122	26.25240875245548	23.92698074809358
13	23.650227006411026	26.360606877937936	26.39103196131194	23.5981341543391
14	23.633144079829258	26.257514663087267	26.40050660041892	23.708834656664557
15	23.354883661603736	26.249761178342002	26.559775595141765	23.835579564912493
16	23.453422452022497	26.24276727912222	26.428601402565395	23.875208866289892
17	23.459729927313603	26.17966331678835	26.301628181511518	24.058978574386533
18	23.497859644430864	26.3532979819917	26.219032786888796	23.92980958668864
19	23.723031779209073	26.130777450856723	26.1765874522163	23.969603317717905
20	23.53985903352584	26.081640993192995	26.163706240332463	24.214793732948703
21	23.5387099113871	26.333150143959422	26.15959054107742	23.968549403576052
22	23.68328293081256	26.13914429722175	26.15438177913243	24.02319099283326
23	23.61324547087755	26.137372706663253	26.290501769288525	23.958880053170663
24	23.525632055994837	26.308873669769323	26.17350075343085	23.99199352080499
25	23.593116454264425	26.305518032150182	26.10995652320438	23.99140899038101
26	23.571196563365227	26.200407193996337	26.231791426266504	23.99660481637193
27	23.793143200851105	26.244863931507425	26.07366134773685	23.888331519904625
28	23.628957626506892	26.191514473399263	25.954850934815564	24.22467696527828
29	23.666138011993343	26.17246559492919	25.88636495993018	24.27503143314729
30	23.853055590344933	26.199788781275757	25.930257408793167	24.016898219586142
31	23.78245905111423	26.22866515518033	26.019471585397618	23.96940420830782
32	23.880625019574982	26.03344712922172	25.9529533648544	24.132974486348896
33	23.704431444221264	26.262347553957156	25.960168859534228	24.073052142287352
34	23.77197230036039	26.153077601423664	26.151194106754456	23.92375599146149
35	23.619571369824207	26.251551495954395	26.002348744501997	24.1265283897194
36	23.817790752930502	26.219265648210992	26.057343247273725	23.905600351584784
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1540.0
1	1950.5
2	2361.0
3	2361.0
4	3657.5
5	4954.0
6	4954.0
7	7704.0
8	10454.0
9	10454.0
10	16702.0
11	22950.0
12	22950.0
13	35831.5
14	48713.0
15	72216.0
16	95719.0
17	95719.0
18	132484.5
19	169250.0
20	169250.0
21	222829.0
22	276408.0
23	276408.0
24	351536.5
25	426665.0
26	532605.0
27	638545.0
28	638545.0
29	782709.0
30	926873.0
31	926873.0
32	1108184.0
33	1289495.0
34	1289495.0
35	1484123.5
36	1678752.0
37	1678752.0
38	1847364.0
39	2015976.0
40	2121702.5
41	2227429.0
42	2227429.0
43	2278881.0
44	2330333.0
45	2330333.0
46	2359657.5
47	2388982.0
48	2388982.0
49	2408098.0
50	2427214.0
51	2431833.0
52	2436452.0
53	2436452.0
54	2387629.0
55	2338806.0
56	2338806.0
57	2207758.0
58	2076710.0
59	2076710.0
60	1804438.5
61	1532167.0
62	1532167.0
63	1266138.0
64	1000109.0
65	798852.0
66	597595.0
67	597595.0
68	468458.5
69	339322.0
70	339322.0
71	262507.0
72	185692.0
73	185692.0
74	143011.5
75	100331.0
76	77795.0
77	55259.0
78	55259.0
79	43795.5
80	32332.0
81	32332.0
82	25690.0
83	19048.0
84	19048.0
85	14671.5
86	10295.0
87	10295.0
88	7592.0
89	4889.0
90	3439.0
91	1989.0
92	1989.0
93	1321.5
94	654.0
95	654.0
96	418.0
97	182.0
98	182.0
99	145.5
100	109.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4271582360661478
2	0.0
3	0.0012592661602997472
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5704223131283296
10	1.0824637481086651E-5
11	0.0
12	0.0
13	0.002089155033849724
14	0.0014577178474530026
15	0.0012953482852367027
16	1.9123526216586418E-4
17	5.4123187405433264E-5
18	0.0
19	2.958734244830352E-4
20	7.577246236760656E-5
21	0.006797872338122417
22	3.6082124936955503E-6
23	8.659709984869321E-5
24	0.0
25	0.0
26	0.0
27	6.855603738021547E-5
28	0.03086464967107174
29	6.494782488651991E-4
30	5.592729365228103E-4
31	0.06565503453528425
32	0.0021865767711795035
33	5.70097574003897E-4
34	4.1133622428129275E-4
35	6.242207614093302E-4
36	1.5876134972260425E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	2.7714554E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.2647368229425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.55525597075786	93.88840522571978
2	0.8492065068645429	1.61798868769562
3	0.19663598918978797	0.5619742728025235
4	0.10147578849244535	0.3866825713853353
5	0.061522597834610886	0.2930467045688968
6	0.044501772465152314	0.25436697852283224
7	0.03443741839903971	0.22964701204522528
8	0.02542086277725139	0.1937369441829591
9	0.019335934671269078	0.1657829454915652
>10	0.10703220744298247	1.7728989452537072
>50	0.004121501647805469	0.26591210000915044
>100	0.0010337432319465212	0.1473474373715608
>500	4.525753122125676E-6	0.0021600348473256132
>1k	3.8029611274387033E-6	0.005879935716172526
>5k	0.0	0.0
>10k+	1.137751102664803E-5	0.21417020438733564
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCG	32383	0.11684474518334302	Illumina Paired End PCR Primer 2 (97% over 36bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.731874234743233E-4	0.0	3.6082124936955508E-6	0.0
2	9.128777609049743E-4	7.2164249873911015E-6	7.2164249873911015E-6	0.0
3	9.88650223272581E-4	1.4432849974782203E-5	1.0824637481086653E-5	0.0
4	0.0010319487731969275	1.4432849974782203E-5	2.5257487455868854E-5	7.2164249873911015E-6
5	0.0011257622980330119	1.8041062468477753E-5	2.8865699949564406E-5	7.2164249873911015E-6
6	0.0011762772729447496	1.8041062468477753E-5	3.608212493695551E-5	7.2164249873911015E-6
7	0.001252049735312356	1.8041062468477753E-5	4.690676241804216E-5	7.2164249873911015E-6
8	0.0013458632601484404	1.8041062468477753E-5	5.051497491173771E-5	7.2164249873911015E-6
9	0.0014757589099214802	2.1649274962173305E-5	6.494782488651992E-5	7.2164249873911015E-6
10	0.0015803970722386511	2.1649274962173305E-5	6.494782488651992E-5	7.2164249873911015E-6
11	0.0018329719467973398	2.1649274962173305E-5	7.216424987391101E-5	7.2164249873911015E-6
12	0.0020530729089127684	2.5257487455868854E-5	8.659709984869322E-5	1.0824637481086653E-5
13	0.0023092559959651524	2.8865699949564406E-5	9.020531234238876E-5	1.4432849974782203E-5
14	0.002514924108105799	2.8865699949564406E-5	1.0102994982347542E-4	1.8041062468477753E-5
15	0.005982416314547223	2.8865699949564406E-5	1.1185458730456207E-4	1.8041062468477753E-5
16	0.006285506164017649	2.8865699949564406E-5	1.1546279979825762E-4	1.8041062468477753E-5
17	0.007137044312529799	2.8865699949564406E-5	1.2628743727934426E-4	1.8041062468477753E-5
18	0.007754048648951738	2.8865699949564406E-5	1.3711207476043092E-4	1.8041062468477753E-5
19	0.00874991529721171	2.8865699949564406E-5	1.4072028725412649E-4	1.8041062468477753E-5
20	0.06988385957789543	2.8865699949564406E-5	1.4432849974782203E-4	1.8041062468477753E-5
21	0.07303743729738534	3.247391244325996E-5	1.4793671224151757E-4	1.8041062468477753E-5
22	0.07424618848277335	3.247391244325996E-5	1.5154492473521314E-4	1.8041062468477753E-5
23	0.0760611193671022	3.9690337430651056E-5	1.623695622162998E-4	1.8041062468477753E-5
24	0.07844975603792867	4.329854992434661E-5	1.623695622162998E-4	1.8041062468477753E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGTTC	6385	0.0	23.548412	11
GAGCGGT	7985	0.0	18.843575	9
AGCGGTT	8100	0.0	18.618011	10
ATGCCGA	7915	0.0	18.591663	25
CGGTTCA	8155	0.0	18.455717	12
AATGCCG	8125	0.0	18.14128	24
GATCGGA	8580	0.0	17.77395	1
AGAGCGG	8500	0.0	17.772627	8
ATCGGAA	8610	0.0	17.67101	2
TCGGAAG	8585	0.0	17.666258	3
AAGAGCG	8645	0.0	17.613525	7
CGGAAGA	8680	0.0	17.438076	4
TGCCGAG	8695	0.0	16.872316	26
GTTCAGC	11745	0.0	12.992853	14
GAATGCC	11630	0.0	12.931539	23
GGTTCAG	12475	0.0	12.4488325	13
CCGAGAC	2975	0.0	12.340793	28
GAGACCG	2920	0.0	12.316642	30
GAAGAGC	13460	0.0	11.625797	6
AGATCGG	4255	0.0	11.5090685	20
>>END_MODULE
