FastQCFastQC Report
Sun 14 Aug 2016
V400_input.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV400_input.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27714554
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCG323830.11684474518334302Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC63850.023.54841211
GAGCGGT79850.018.8435759
AGCGGTT81000.018.61801110
ATGCCGA79150.018.59166325
CGGTTCA81550.018.45571712
AATGCCG81250.018.1412824
GATCGGA85800.017.773951
AGAGCGG85000.017.7726278
ATCGGAA86100.017.671012
TCGGAAG85850.017.6662583
AAGAGCG86450.017.6135257
CGGAAGA86800.017.4380764
TGCCGAG86950.016.87231626
GTTCAGC117450.012.99285314
GAATGCC116300.012.93153923
GGTTCAG124750.012.448832513
CCGAGAC29750.012.34079328
GAGACCG29200.012.31664230
GAAGAGC134600.011.6257976
AGATCGG42550.011.509068520
Produced by FastQC (version 0.11.3)