##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	V400_H3K4me3_ChIPSeq.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34282858
Sequences flagged as poor quality	0
Sequence length	36
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.499939969999	39.0	35.0	40.0	20.0	40.0
2	33.96628072840368	39.0	35.0	40.0	10.0	40.0
3	33.99034412475179	39.0	35.0	40.0	8.0	40.0
4	33.85654629494425	39.0	35.0	40.0	2.0	40.0
5	33.67158595704011	39.0	35.0	40.0	2.0	40.0
6	33.471091879212636	39.0	35.0	40.0	2.0	40.0
7	33.49668055679605	39.0	35.0	40.0	2.0	40.0
8	32.66602906910503	39.0	34.0	40.0	2.0	40.0
9	33.11167254492026	39.0	34.0	40.0	2.0	40.0
10	33.38042222150791	39.0	35.0	40.0	2.0	40.0
11	33.445148272060635	39.0	35.0	40.0	2.0	40.0
12	33.47917726112566	39.0	35.0	40.0	2.0	40.0
13	33.421022774705655	39.0	35.0	40.0	2.0	40.0
14	32.71054738201815	39.0	33.0	40.0	2.0	40.0
15	33.07274781466586	39.0	34.0	40.0	2.0	40.0
16	33.3498620214219	39.0	35.0	40.0	2.0	40.0
17	32.70579809886328	39.0	33.0	40.0	2.0	40.0
18	32.99502033931943	39.0	34.0	40.0	2.0	40.0
19	33.16021706825026	39.0	34.0	40.0	2.0	40.0
20	33.02008035619434	39.0	34.0	40.0	2.0	40.0
21	33.1679818817906	39.0	35.0	40.0	2.0	40.0
22	32.993446899905486	39.0	34.0	40.0	2.0	40.0
23	32.971896421237695	39.0	34.0	40.0	2.0	40.0
24	32.89298920177542	39.0	34.0	40.0	2.0	40.0
25	32.79483589145339	39.0	33.0	40.0	2.0	40.0
26	32.46988527035873	39.0	33.0	40.0	2.0	40.0
27	32.37601156239658	38.0	33.0	40.0	2.0	40.0
28	31.790820356925902	38.0	32.0	40.0	2.0	40.0
29	32.06722773813082	38.0	33.0	40.0	2.0	40.0
30	31.891983042954003	38.0	32.0	40.0	2.0	40.0
31	31.4285925053273	38.0	32.0	40.0	2.0	40.0
32	31.54466561685143	38.0	32.0	40.0	2.0	40.0
33	31.561664753854537	38.0	32.0	40.0	2.0	40.0
34	31.602726645485625	38.0	32.0	40.0	2.0	40.0
35	31.49837070176588	38.0	32.0	40.0	2.0	40.0
36	31.33755948818503	38.0	32.0	40.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-2.092064161738172
1	2	-1.0282932000804124
1	3	-0.9172920416856627
1	4	-0.9528998742601189
1	5	-0.5169829747294798
1	6	-0.6501476811946105
1	7	-0.5814010939157228
1	8	0.186109576130832
1	9	-2.1328766851128904
1	10	-0.6471319284169539
1	11	-0.32044203840077756
1	12	-0.41810900458460765
1	13	-0.4184699986419034
1	14	0.31360982839315454
1	15	-0.13285480684706386
1	16	-0.011384266407269195
1	17	0.453927061531445
1	18	0.24937780221973327
1	19	-0.042350930595567604
1	20	0.13653945515569177
1	21	-0.01128085733307671
1	22	0.06291490128060673
1	23	-0.0037789817746372023
1	24	0.038834628743366295
1	25	0.19862033298777249
1	26	0.5306477664983973
1	27	0.5723992833734997
1	28	1.147296992936031
1	29	0.7283061280025152
1	30	0.9118095489573292
1	31	1.2562456391474512
1	32	1.0559320409510171
1	33	0.8366880880074667
1	34	0.6910267963947803
1	35	0.7167624634839775
1	36	0.9084731617900879
2	1	0.1859294853636726
2	2	0.0867514718849165
2	3	0.24021330710174027
2	4	0.06773459505253498
2	5	0.501915340244004
2	6	0.7837603535147011
2	7	0.668064292838956
2	8	1.2913247606680827
2	9	0.9507355528957007
2	10	0.6403825333069477
2	11	0.6044938988499666
2	12	0.575391650150884
2	13	0.5913745689095009
2	14	1.246702519392315
2	15	0.632459454590574
2	16	0.6328491442643909
2	17	1.1819346949166913
2	18	0.8296641582404334
2	19	0.6344571761911766
2	20	0.7117907083090387
2	21	0.5360577124791348
2	22	0.5023669440050327
2	23	0.557504233626581
2	24	0.5603154965271813
2	25	0.6168643062156676
2	26	0.6209358264259919
2	27	0.6326699738745347
2	28	1.0337151051869178
2	29	0.5871489440376294
2	30	0.8504107573996649
2	31	0.9606830561128277
2	32	1.0129632718047326
2	33	0.6479463509510985
2	34	0.6591804065174856
2	35	0.6963457573606568
2	36	0.7507177451829712
3	1	0.04040248499153165
3	2	-0.02466744626430284
3	3	0.0659391603309416
3	4	0.1958300682799603
3	5	0.37384620585059736
3	6	0.5569828021919321
3	7	0.48994275563191536
3	8	0.9651918911145785
3	9	0.7293138749433368
3	10	0.45078544800876585
3	11	0.408822096567377
3	12	0.4602695697586867
3	13	0.47474630293547904
3	14	1.1399000624522273
3	15	0.6651897339029205
3	16	0.5026373293729378
3	17	1.1286174191516878
3	18	0.7487432876977209
3	19	0.5356269002433152
3	20	0.5733695759923378
3	21	0.43004447678247715
3	22	0.5148820792105511
3	23	0.486990936236694
3	24	0.5181324071332085
3	25	0.5407305259999831
3	26	0.5407563466651126
3	27	0.5081914645748924
3	28	0.9769504018174082
3	29	0.59168611968542
3	30	0.8316350349183708
3	31	1.0690109011887259
3	32	0.9697065448094015
3	33	0.8049420032180201
3	34	0.6771808657230309
3	35	0.7323396636729207
3	36	0.77827362954093
4	1	-0.0616352789231982
4	2	-0.0957725412411321
4	3	0.05146716067864787
4	4	0.10789564896631987
4	5	0.3362096757767432
4	6	0.6145001467629996
4	7	0.4841120812860922
4	8	1.099601954574858
4	9	0.7784943953847474
4	10	0.51389678478143
4	11	0.4182061616852337
4	12	0.4154034282440975
4	13	0.4517051926873421
4	14	1.1419089626704135
4	15	0.5799296576671296
4	16	0.4674748983104564
4	17	1.0993846028681489
4	18	0.7035709809281911
4	19	0.5149003447323821
4	20	0.5826293669193205
4	21	0.4168190851424285
4	22	0.39158356768509606
4	23	0.46088396405416177
4	24	0.46625828845095185
4	25	0.4485578923008475
4	26	0.5712595808836056
4	27	0.608935735338143
4	28	0.7417817669879945
4	29	0.6280475577533409
4	30	0.7776164574935791
4	31	1.1290838511079144
4	32	0.8387168777423248
4	33	0.8300378543267435
4	34	0.6897611099768781
4	35	0.5737850387200858
4	36	0.8269775873148255
5	1	0.12860893284791075
5	2	0.028432152716575843
5	3	0.1018791899719318
5	4	0.19763355784893122
5	5	0.3452948440104038
5	6	0.6266068372829423
5	7	0.5642416539966035
5	8	1.0862651427298289
5	9	0.8387272135849742
5	10	0.5139157465455853
5	11	0.5379837197784454
5	12	0.4755612588354339
5	13	0.5173811345035375
5	14	1.1609488128903038
5	15	0.6870584219660714
5	16	0.45708345089848024
5	17	1.1559675124509283
5	18	0.7651082917548351
5	19	0.5433367031541252
5	20	0.6035083370073764
5	21	0.3716660684204669
5	22	0.4701586129594375
5	23	0.35088773532779527
5	24	0.4310837021699143
5	25	0.4039669047211092
5	26	0.3851149285291342
5	27	0.4732351940976969
5	28	0.7232054836437491
5	29	0.6439690366027335
5	30	0.7354291348967266
5	31	1.0504012353236973
5	32	0.6314826662874431
5	33	0.7652720930471553
5	34	0.7575964782171383
5	35	0.7022694198683261
5	36	0.8060315211030478
6	1	-0.13309211420460088
6	2	-0.2464591571962984
6	3	-0.2517542776266808
6	4	-0.09253116640893211
6	5	0.09950564154905805
6	6	0.40197558686235624
6	7	0.3194600898532798
6	8	0.8913826543117764
6	9	0.6451216305833043
6	10	0.30750919728040316
6	11	0.2358351619132364
6	12	0.18553101463634647
6	13	0.2388762764102097
6	14	0.9819967547729433
6	15	0.49924897367291265
6	16	0.2241563808953444
6	17	0.8996567650523986
6	18	0.5392648960342896
6	19	0.3351233279221688
6	20	0.3805314457722204
6	21	0.17157569754714785
6	22	0.22426428186974334
6	23	0.26242885617909906
6	24	0.25826001436309554
6	25	0.2855638462149912
6	26	0.20593949961922675
6	27	0.252391397023807
6	28	0.5421817596166214
6	29	0.10032722034533492
6	30	0.3257758925738159
6	31	0.8904530502196266
6	32	0.758681265287759
6	33	0.6716454935185432
6	34	0.4990567452635375
6	35	0.44015299247630324
6	36	0.6316524192684589
7	1	-0.002534828313663695
7	2	-0.11989599694901898
7	3	-0.01296952081114
7	4	0.08911416040690057
7	5	0.2860025262344834
7	6	0.5592146995015739
7	7	0.4197101157685452
7	8	1.0242775825629238
7	9	0.7393038291564906
7	10	0.4008300585653686
7	11	0.3764672326363723
7	12	0.29548710050650584
7	13	0.3636372228111142
7	14	1.0414369351685124
7	15	0.39437880784059587
7	16	0.3828901618130942
7	17	1.0218039717743181
7	18	0.6144496936130963
7	19	0.43373458129375564
7	20	0.4844325686078008
7	21	0.3354253210832354
7	22	0.3432995694345422
7	23	0.37961236839187507
7	24	0.33800646681524427
7	25	0.36638699543473763
7	26	0.454770384666773
7	27	0.4400655492501997
7	28	0.8872118030495493
7	29	0.5257694238553299
7	30	0.5851161540135479
7	31	0.9708623764484869
7	32	0.8603576906824024
7	33	0.726999459565068
7	34	0.5541499128762837
7	35	0.6740245933654556
7	36	0.6655965517396503
8	1	0.054423766038617316
8	2	-0.005145580153808282
8	3	-0.07391177284131345
8	4	0.040448875209968094
8	5	0.3214929087766336
8	6	0.48119662760001347
8	7	0.4636307779749558
8	8	0.9765978621446081
8	9	0.8020603004899698
8	10	0.48796677485177753
8	11	0.40517927876932447
8	12	0.4097883990092228
8	13	0.4016153822258204
8	14	1.0858021791520258
8	15	0.6446210332979945
8	16	0.45369930102882705
8	17	1.0230045704334998
8	18	0.6267672395383386
8	19	0.4492434083289041
8	20	0.5029183784350764
8	21	0.33634536737731224
8	22	0.3132399755432047
8	23	0.36330697589487926
8	24	0.3704713455746145
8	25	0.40401684461974696
8	26	0.5241841111464893
8	27	0.46102235095205657
8	28	0.920727506807804
8	29	0.3618098728911079
8	30	0.5749367321928602
8	31	0.7243576043617637
8	32	0.7834745420354317
8	33	0.7392239126638636
8	34	0.5880616371012479
8	35	0.6047263186414931
8	36	0.6100999619847265
9	1	0.08008305878549038
9	2	-0.005082630244785946
9	3	0.13935372245211397
9	4	0.23421210376044854
9	5	0.4296831814111357
9	6	0.6320762971085401
9	7	0.528926877621231
9	8	1.1342855678679342
9	9	0.8747649980575787
9	10	0.45453784707225253
9	11	0.4749411060908102
9	12	0.425862042885754
9	13	0.4768995235327296
9	14	1.1498634282751254
9	15	0.6404807719073773
9	16	0.49500885645572623
9	17	1.0145169007328505
9	18	0.6999871957532662
9	19	0.4783655300249663
9	20	0.5523841413716397
9	21	0.3882941901040553
9	22	0.36540205919859403
9	23	0.46312422497676664
9	24	0.465465365300453
9	25	0.47139968153334877
9	26	0.6300542906049316
9	27	0.47358332976016726
9	28	0.7686791495198797
9	29	0.4138591639569782
9	30	0.5765410751161042
9	31	0.6850381846376408
9	32	0.8068006770259792
9	33	0.7244006487408576
9	34	0.5217154621107696
9	35	0.5838988002100898
9	36	0.6303752512046721
10	1	0.06412453414160524
10	2	-0.05246824448563103
10	3	0.0042914744792383885
10	4	0.17935964669925397
10	5	0.35269507977918124
10	6	0.5981614930269785
10	7	0.518725107752914
10	8	0.9995532613451346
10	9	0.7777262251861288
10	10	0.47898204385762
10	11	0.42651635508676833
10	12	0.4447861948241041
10	13	0.4194356427921946
10	14	1.1036603103316693
10	15	0.6472365262547086
10	16	0.48665951494192683
10	17	1.1041226875274504
10	18	0.7350949602434156
10	19	0.47539334381194465
10	20	0.5576589889900703
10	21	0.36467647979430495
10	22	0.32454124700413445
10	23	0.35595146506651787
10	24	0.39806742621436797
10	25	0.37873820696560045
10	26	0.3613073172461867
10	27	0.2992293502445307
10	28	0.9365219984654338
10	29	0.33365005986610186
10	30	0.5099793095407321
10	31	0.8347106454643978
10	32	0.623330203573996
10	33	0.751959556962273
10	34	0.6495611198892561
10	35	0.6210727164998424
10	36	0.687082986266315
11	1	0.16584409282300783
11	2	0.13957881555991491
11	3	0.2438239062140184
11	4	0.21196442320506037
11	5	0.4710821537873926
11	6	0.6515146358703348
11	7	0.5614277206983829
11	8	1.1371667567304158
11	9	0.8671802911934989
11	10	0.5228509340438521
11	11	0.5085068523917897
11	12	0.40124534218200836
11	13	0.5112563862481352
11	14	1.160145697028618
11	15	0.6343729039077033
11	16	0.5392719465454547
11	17	1.123987814359836
11	18	0.8038075458033944
11	19	0.5809007983543708
11	20	0.645607030534805
11	21	0.4000930880583269
11	22	0.38528546515087214
11	23	0.45196051206681176
11	24	0.480486840853402
11	25	0.4589330093644577
11	26	0.2800853479119141
11	27	0.47977857610574404
11	28	0.966214829632488
11	29	0.5789640375518488
11	30	0.652949710989958
11	31	0.8782491928157512
11	32	0.8575026843954809
11	33	0.7975850920283385
11	34	0.7154065866890633
11	35	0.7223885628036406
11	36	0.7482043226633586
12	1	0.07418878742947044
12	2	0.11505921892964466
12	3	0.1951345594709437
12	4	0.22513595469403924
12	5	0.4106484406260904
12	6	0.6339812882874298
12	7	0.5771590989357094
12	8	1.1313363551411797
12	9	0.9321942646648651
12	10	0.5326034610909787
12	11	0.44550098608122113
12	12	0.48140195446144673
12	13	0.5111756935312641
12	14	1.1815071304850946
12	15	0.6901035290667679
12	16	0.5116988045889741
12	17	1.1269446097367535
12	18	0.8012182199161302
12	19	0.5653156107072093
12	20	0.646836337843304
12	21	0.48155790838629997
12	22	0.4439637981243223
12	23	0.5388563807465374
12	24	0.5374367617703868
12	25	0.5854421499554974
12	26	0.5593800271737379
12	27	0.546771913429474
12	28	0.9156826486320959
12	29	0.4802081426690563
12	30	0.5514851154430787
12	31	0.9904739820634489
12	32	0.851210736772746
12	33	0.8658100516706391
12	34	0.7464034739860992
12	35	0.7485677931794932
12	36	0.8028436843293996
13	1	0.11677702896693631
13	2	0.02006946637676066
13	3	0.1925050079096522
13	4	0.18570805727004824
13	5	0.48940412663188226
13	6	0.6344905707073281
13	7	0.5390822937087236
13	8	1.184273796838859
13	9	0.9038181993499563
13	10	0.5067803237020669
13	11	0.4718963474189408
13	12	0.5085683853822403
13	13	0.48990611721470856
13	14	1.204305393101052
13	15	0.7119747487969548
13	16	0.5747142344850928
13	17	1.1586694439940288
13	18	0.7480499655102406
13	19	0.5648762768705424
13	20	0.6427445625421981
13	21	0.4810896982585575
13	22	0.36789883453005956
13	23	0.4800405162158867
13	24	0.49683199880036
13	25	0.5720389333552944
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118	9	0.9629295652667196
118	10	0.6406263485689934
118	11	0.6337229956178234
118	12	0.5953913756739979
118	13	0.583891159176197
118	14	1.2583715452493607
118	15	0.8793462848453615
118	16	0.5425079272963558
118	17	1.2120318228321736
118	18	0.8315280473064846
118	19	0.5631624256574028
118	20	0.676390796286995
118	21	0.5011025444717419
118	22	0.5700432822042316
118	23	0.5178987596755675
118	24	0.5550226974360797
118	25	0.5613989676322362
118	26	0.6655723006804521
118	27	0.7018893462144504
118	28	1.1121708721983374
118	29	0.8370196899708517
118	30	0.8112113198826734
118	31	1.0951402748720085
118	32	0.9041449079005055
118	33	0.8375227567173162
118	34	0.7610837565826287
118	35	0.6835162480150281
118	36	0.8287667288020764
119	1	0.10425155290062804
119	2	0.12897521801623668
119	3	0.22978929064103681
119	4	0.2296015349092997
119	5	0.5658993626656894
119	6	0.7314671215265491
119	7	0.6423897556950138
119	8	1.4543063711074282
119	9	1.0055371308143535
119	10	0.6587254456184155
119	11	0.6681201402825607
119	12	0.5996121592514427
119	13	0.6236753351341804
119	14	1.2651729476643538
119	15	0.8858969775748733
119	16	0.5784287015831779
119	17	1.1782838746766657
119	18	0.789339504311215
119	19	0.5837373913189552
119	20	0.643043501013544
119	21	0.4801152994797775
119	22	0.5676291959521151
119	23	0.5134003191120726
119	24	0.5391199470965873
119	25	0.5685609080411922
119	26	0.6148074717417131
119	27	0.6858606239860663
119	28	1.0500218125783753
119	29	0.6115549957937034
119	30	0.8061957543032747
119	31	1.0311212918558752
119	32	0.9444159942800603
119	33	0.8527515371783174
119	34	0.7415154962886774
119	35	0.7191479455336172
119	36	0.817843391239645
120	1	-0.05478396044404121
120	2	0.07655824702604264
120	3	-0.006152527729000212
120	4	0.05528812133299965
120	5	0.34469781052391824
120	6	0.5179204265744133
120	7	0.4078182924437428
120	8	1.0985503632317162
120	9	0.733325098973495
120	10	0.42861820888161617
120	11	0.39309672181865096
120	12	0.357111072217009
120	13	0.39165298824354267
120	14	1.021411642254563
120	15	0.6385125161318967
120	16	0.35141297099364976
120	17	0.9973928452271323
120	18	0.6125355765166276
120	19	0.4052365844964356
120	20	0.45927650354389726
120	21	0.28448093734295554
120	22	0.3695152248317086
120	23	0.30010099977601357
120	24	0.30497178843009465
120	25	0.36732582432075844
120	26	0.45129109733924366
120	27	0.3961551349091721
120	28	0.7626369833159323
120	29	0.6148650744329132
120	30	0.6777232801324686
120	31	0.8332143548608215
120	32	0.8465539576170507
120	33	0.7336922888767354
120	34	0.6078638943986405
120	35	0.6193637525716262
120	36	0.7175535466735745
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3330103.0
3	67823.0
4	99276.0
5	194706.0
6	65552.0
7	52761.0
8	58376.0
9	58414.0
10	59148.0
11	49513.0
12	52699.0
13	59139.0
14	62184.0
15	62638.0
16	65667.0
17	71339.0
18	77380.0
19	83742.0
20	94806.0
21	114838.0
22	139587.0
23	159660.0
24	177628.0
25	205493.0
26	245550.0
27	297204.0
28	345856.0
29	338597.0
30	364018.0
31	442807.0
32	548158.0
33	699156.0
34	951315.0
35	1377204.0
36	2079309.0
37	3337065.0
38	4408397.0
39	1.3050019E7
40	335731.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.66197415523596	21.02224493235876	13.626241051990103	31.689539860415174
2	32.48200704041069	22.218187311135516	19.201789547734236	26.09801610071955
3	30.309235787467443	20.719793824935557	19.86640124976796	29.104569137829035
4	32.13348966192856	20.46515112541652	18.677505925884702	28.72385328677022
5	32.309505820646876	21.41826437938497	19.400584073424554	26.8716457265436
6	32.0334334653593	20.222384265341066	17.50058012452799	30.243602144771643
7	30.618730978506857	21.03100674902555	19.51538024798808	28.83488202447951
8	30.80829205930099	19.964528719597883	19.550444970437976	29.676734250663152
9	30.98356025549915	19.23765287140049	19.32867465854872	30.450112214551645
10	30.751545482938948	21.13670547338981	18.365144907160154	29.746604136511095
11	32.293153946677094	19.618361213601307	18.60569790573761	29.482786933983984
12	31.3718394223573	19.389311205855588	18.97722841934453	30.26162095244258
13	31.170604415894836	19.998953320913724	19.185583425556572	29.644858837634864
14	30.947029268329423	20.267510208851235	19.101754931813602	29.683705591005737
15	30.22036387169795	19.58382709567033	20.38081386468878	29.814995167942946
16	30.893000253379604	19.46326221647122	19.706853293741432	29.936884236407746
17	30.479210603870843	19.616957115128375	19.01905686097922	30.88477542002156
18	30.423064762903696	20.13966401823602	19.008353871926182	30.4289173469341
19	31.77452837082262	19.402324314327746	18.975435867568084	29.847711447281544
20	30.611835920182095	20.038365234149826	18.89050284629869	30.45929599936939
21	31.004688573223323	20.111057342656107	18.901700726877078	29.982553357243496
22	31.10595123962731	19.968133524383962	18.947855469288154	29.978059766700575
23	30.92391105632677	19.548769298589303	19.54764699138086	29.97967265370307
24	30.444673343021567	20.09361721057185	19.03943464004935	30.42227480635723
25	31.027670525582785	20.19437528885832	18.89696012656529	29.880994058993604
26	31.177824005549144	19.650273073871556	19.612058448208433	29.559844472370873
27	31.389091976911367	20.03040428833777	19.018380723347178	29.562123011403692
28	30.910560012086385	20.069458094320435	18.878182237238843	30.141799656354333
29	31.378002690893908	19.54647485942832	18.965435228453075	30.110087221224703
30	31.55017410964782	20.065786216457074	18.795333932166784	29.588705741728315
31	31.538799227870857	20.17725505996226	18.801807858193097	29.48213785397379
32	31.498872676820916	19.636549757396658	18.918757692155566	29.94581987362686
33	31.189427891821335	20.36869119729446	18.844801926076737	29.597078984807467
34	31.219279175218457	19.515598126853213	19.344800604243943	29.920322093684394
35	30.724738064599176	19.649290337235428	19.364894195151933	30.261077403013463
36	31.323892615531108	19.797722480736894	19.283471887014343	29.594913016717655
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	95971.0
1	83494.5
2	71018.0
3	71018.0
4	47924.0
5	24830.0
6	24830.0
7	25407.5
8	25985.0
9	25985.0
10	27021.0
11	28057.0
12	28057.0
13	30842.0
14	33627.0
15	38034.0
16	42441.0
17	42441.0
18	49601.0
19	56761.0
20	56761.0
21	70497.0
22	84233.0
23	84233.0
24	107204.5
25	130176.0
26	164677.5
27	199179.0
28	199179.0
29	249267.0
30	299355.0
31	299355.0
32	363286.0
33	427217.0
34	427217.0
35	506141.5
36	585066.0
37	585066.0
38	676610.0
39	768154.0
40	869462.5
41	970771.0
42	970771.0
43	1084681.0
44	1198591.0
45	1198591.0
46	1329803.5
47	1461016.0
48	1461016.0
49	1619605.0
50	1778194.0
51	1947484.0
52	2116774.0
53	2116774.0
54	2296254.0
55	2475734.0
56	2475734.0
57	2721671.0
58	2967608.0
59	2967608.0
60	2910191.5
61	2852775.0
62	2852775.0
63	2780108.5
64	2707442.0
65	2633427.0
66	2559412.0
67	2559412.0
68	2453692.0
69	2347972.0
70	2347972.0
71	2220518.0
72	2093064.0
73	2093064.0
74	1944869.0
75	1796674.0
76	1626810.5
77	1456947.0
78	1456947.0
79	1276318.5
80	1095690.0
81	1095690.0
82	917565.0
83	739440.0
84	739440.0
85	588065.5
86	436691.0
87	436691.0
88	329278.5
89	221866.0
90	157398.0
91	92930.0
92	92930.0
93	62334.5
94	31739.0
95	31739.0
96	19935.5
97	8132.0
98	8132.0
99	4729.0
100	1326.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0149037166037906
2	1.0164671801866694
3	0.9896403619558206
4	0.9912388284547338
5	1.1112142400729834
6	1.1354741777946284
7	1.0454350101149676
8	3.596613794567536
9	1.8166075885505228
10	1.0787723707282515
11	1.1515755191705428
12	1.1911317311993066
13	1.0677727043643794
14	2.798976094700156
15	1.9327472639533143
16	1.21540333655963
17	1.8852308054363494
18	1.9653320618718544
19	1.1223539181009938
20	1.1244745114307564
21	1.1152891628813444
22	1.081385921792168
23	1.2368747086371854
24	1.118115648351138
25	1.102090146626632
26	1.0767509523272534
27	1.1441403164228607
28	1.8041990548162583
29	1.027119734299865
30	1.0215542706503642
31	1.887339731127434
32	1.032037644002726
33	1.0265042663595898
34	1.0234473450259018
35	1.0185994411551103
36	1.0231439864202687
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	3.4282858E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.4118040519761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.87749729790316	66.6086337979124
2	11.52149307028687	18.29885100167643
3	3.1572166774290533	7.521608164128812
4	0.9110810872940457	2.8940237111862435
5	0.27740939030153683	1.10147900724019
6	0.1038912627024771	0.49501155578648903
7	0.04628864721822494	0.2573105487907309
8	0.023178600429981728	0.14725235804358064
9	0.016172358374782318	0.11558485388848933
>10	0.06143983381962331	0.8482726593917097
>50	0.0030949214620730977	0.16420232255013884
>100	0.0011592359396942612	0.14783569635722807
>500	2.2007754020100065E-5	0.011087279946116205
>1k	1.8825891340847753E-5	0.02917955022225292
>5k	3.678319500331651E-6	0.016419052711804574
>10k+	3.3104873654211245E-5	1.3432484401672753
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT	128473	0.37474413597606127	Illumina Paired End PCR Primer 2 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCG	104161	0.30382822808996846	Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG	68310	0.19925409952694143	Illumina Paired End PCR Primer 2 (100% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	57823	0.1686644678223735	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010384198423597005	2.9169096695497208E-6	8.750729008649162E-6	0.0
2	0.0012396866095586313	5.8338193390994416E-6	1.1667638678198883E-5	2.9169096695497208E-6
3	0.001712225976025686	5.8338193390994416E-6	1.1667638678198883E-5	1.4584548347748604E-5
4	0.002059338226702103	8.750729008649162E-6	1.1667638678198883E-5	2.6252187025947486E-5
5	0.002581465057551503	8.750729008649162E-6	1.4584548347748604E-5	2.916909669549721E-5
6	0.0028848236631846737	8.750729008649162E-6	1.4584548347748604E-5	4.0836735373696094E-5
7	0.0032756895589043365	8.750729008649162E-6	1.7501458017298324E-5	4.958746438234525E-5
8	0.003663638544954449	8.750729008649162E-6	1.7501458017298324E-5	5.542128372144469E-5
9	0.004112842634065106	1.4584548347748604E-5	2.0418367686848047E-5	5.833819339099442E-5
10	0.004634969464914506	1.4584548347748604E-5	2.3335277356397766E-5	7.292274173874302E-5
11	0.0054283688950320305	1.4584548347748604E-5	2.3335277356397766E-5	8.167347074739219E-5
12	0.006341361621601093	1.4584548347748604E-5	2.3335277356397766E-5	8.750729008649163E-5
13	0.0071697639677532135	1.7501458017298324E-5	2.3335277356397766E-5	9.625801909514078E-5
14	0.007861071559436498	1.7501458017298324E-5	2.916909669549721E-5	1.0209183843424023E-4
15	0.027911908627921277	1.7501458017298324E-5	2.916909669549721E-5	1.1667638678198883E-4
16	0.029419950927078484	1.7501458017298324E-5	3.208600636504693E-5	1.283440254601877E-4
17	0.032103507823064224	1.7501458017298324E-5	4.375364504324581E-5	1.3709475446883686E-4
18	0.03445745392639085	1.7501458017298324E-5	4.375364504324581E-5	1.4292857380793632E-4
19	0.03808317264564116	2.0418367686848047E-5	4.375364504324581E-5	1.4876239314703577E-4
20	0.47859778785070956	2.0418367686848047E-5	4.375364504324581E-5	1.6334694149478437E-4
21	0.4889090635325678	2.0418367686848047E-5	4.375364504324581E-5	1.7793148984253298E-4
22	0.49214975017543755	2.0418367686848047E-5	4.375364504324581E-5	1.8959912852073186E-4
23	0.49647552721537974	2.0418367686848047E-5	4.375364504324581E-5	2.0126676719893073E-4
24	0.5026739602631729	2.0418367686848047E-5	4.375364504324581E-5	2.1001749620757989E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGTTC	27225	0.0	22.642086	11
CTCGTAT	2875	0.0	20.472103	28
TCGTATG	3085	0.0	19.4014	29
TCTCGTA	3085	0.0	19.012213	27
GACCGAT	3380	0.0	17.739708	21
AGATCGG	27305	0.0	17.577158	20
ACCGATC	3555	0.0	16.667236	22
ATCTCGT	3585	0.0	16.408197	26
AGCGGTT	41275	0.0	15.026685	10
CGTATGC	4090	0.0	14.6734295	30
ATGCCGA	42075	0.0	14.659128	25
CGGTTCA	42355	0.0	14.540408	12
AATGCCG	42625	0.0	14.519761	24
AAGAGCG	44040	0.0	14.43322	7
ATCGGAA	43510	0.0	14.339938	2
GAGCGGT	43825	0.0	14.222865	9
TCGGAAG	44815	0.0	14.058996	3
GAGACCG	17710	0.0	14.046142	30
CGGAAGA	45650	0.0	13.868475	4
GAATGCC	44895	0.0	13.847556	23
>>END_MODULE
