FastQCFastQC Report
Sun 14 Aug 2016
V400_H3K4me3_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV400_H3K4me3_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34282858
Sequences flagged as poor quality0
Sequence length36
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT1284730.37474413597606127Illumina Paired End PCR Primer 2 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCG1041610.30382822808996846Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG683100.19925409952694143Illumina Paired End PCR Primer 2 (100% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN578230.1686644678223735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC272250.022.64208611
CTCGTAT28750.020.47210328
TCGTATG30850.019.401429
TCTCGTA30850.019.01221327
GACCGAT33800.017.73970821
AGATCGG273050.017.57715820
ACCGATC35550.016.66723622
ATCTCGT35850.016.40819726
AGCGGTT412750.015.02668510
CGTATGC40900.014.673429530
ATGCCGA420750.014.65912825
CGGTTCA423550.014.54040812
AATGCCG426250.014.51976124
AAGAGCG440400.014.433227
ATCGGAA435100.014.3399382
GAGCGGT438250.014.2228659
TCGGAAG448150.014.0589963
GAGACCG177100.014.04614230
CGGAAGA456500.013.8684754
GAATGCC448950.013.84755623
Produced by FastQC (version 0.11.3)