FastQCFastQC Report
Sun 14 Aug 2016
V400_H3K4me1_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV400_H3K4me1_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15749589
Sequences flagged as poor quality0
Sequence length142
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199870.12690489891513995No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAT45500.032.06012370-71
AGCGGTC60700.030.36196570-71
GATCGAC19100.029.95411372-73
AGCGGTA46700.029.4160170-71
ATCGGAG85550.027.86232270-71
GATCGAA28700.025.8675472-73
CGGTTCG16750.025.57849770-71
GATCGAT22750.024.25013472-73
CGGGTCG28900.022.35501970-71
TCGGACC30050.022.32555872-73
ATCGGTC17850.021.94017672-73
TCGGACA36750.021.59131472-73
GCGGTTA32450.021.3764170-71
GATCGTT28150.020.80801272-73
ATCGGTG32100.020.687672-73
CGAGACG33900.020.56238470-71
TCGGATC24000.020.43298372-73
AGATCGA105450.020.4115770-71
TCGGACT35200.020.31688772-73
GCGGTAA23400.019.93816872-73
Produced by FastQC (version 0.11.3)