##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	V400_H3K27ac_ChIPSeq.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31141437
Sequences flagged as poor quality	0
Sequence length	36
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.46285648282704	40.0	37.0	40.0	2.0	40.0
2	33.79438241722757	40.0	36.0	40.0	2.0	40.0
3	33.700689727323756	40.0	36.0	40.0	2.0	40.0
4	33.65715374663025	40.0	36.0	40.0	2.0	40.0
5	33.602284795014434	39.0	36.0	40.0	2.0	40.0
6	33.451959651059134	40.0	36.0	40.0	2.0	40.0
7	33.24309469084551	39.0	35.0	40.0	2.0	40.0
8	33.391066089853204	39.0	36.0	40.0	2.0	40.0
9	33.357935377227456	39.0	36.0	40.0	2.0	40.0
10	33.27637536443807	39.0	35.0	40.0	2.0	40.0
11	33.477982695532	39.0	36.0	40.0	2.0	40.0
12	33.48789723480005	39.0	36.0	40.0	2.0	40.0
13	33.46762562690989	39.0	36.0	40.0	2.0	40.0
14	33.47627670489322	39.0	36.0	40.0	2.0	40.0
15	33.45711943222145	39.0	36.0	40.0	2.0	40.0
16	33.385237135974165	39.0	36.0	40.0	2.0	40.0
17	33.51792028736503	39.0	36.0	40.0	2.0	40.0
18	33.48945409937249	39.0	35.0	40.0	2.0	40.0
19	33.461056019990345	39.0	35.0	40.0	2.0	40.0
20	33.39501520112896	39.0	35.0	40.0	2.0	40.0
21	33.383118672397806	39.0	35.0	40.0	2.0	40.0
22	33.29978953765043	39.0	35.0	40.0	2.0	40.0
23	33.19985346212508	39.0	35.0	40.0	2.0	40.0
24	33.189798627468605	39.0	35.0	40.0	2.0	40.0
25	33.13800182053256	39.0	35.0	40.0	2.0	40.0
26	32.9786144743417	39.0	35.0	40.0	2.0	40.0
27	32.811063567811594	39.0	35.0	40.0	2.0	40.0
28	32.713508243052495	39.0	34.0	40.0	2.0	40.0
29	32.735740261440085	39.0	34.0	40.0	2.0	40.0
30	32.47812183490441	39.0	33.0	40.0	2.0	40.0
31	32.3811795839736	39.0	33.0	40.0	2.0	40.0
32	32.308961721965495	39.0	33.0	40.0	2.0	40.0
33	32.04314974289722	38.0	33.0	40.0	2.0	40.0
34	32.00298454435484	38.0	33.0	40.0	2.0	40.0
35	32.276485860302465	39.0	33.0	40.0	2.0	40.0
36	32.16349361142198	39.0	33.0	40.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	0.16671129329979095
1	2	0.15726906828249554
1	3	0.18757433886472086
1	4	0.18154545411532297
1	5	0.25394673503141973
1	6	0.22316476632069282
1	7	0.11695207350227577
1	8	0.2530007121550426
1	9	0.26504046714366325
1	10	0.30243297534999414
1	11	0.29796202533010785
1	12	0.2931091615243133
1	13	0.29388709398069324
1	14	0.2639108691719585
1	15	0.24916678708717654
1	16	0.3436883395116368
1	17	0.13070963455560758
1	18	0.142393771077856
1	19	0.10312006198097379
1	20	0.12519890983598003
1	21	0.0987479994222511
1	22	0.03775861776645684
1	23	0.019748986233167898
1	24	0.0327466615151053
1	25	0.11552292854267421
1	26	0.0962937870309446
1	27	0.05738259437860904
1	28	0.1001188005454523
1	29	0.0793318581251441
1	30	0.20359492201859553
1	31	0.03905567904848084
1	32	0.13331436840584132
1	33	0.2173823781905071
1	34	0.029021642395150593
1	35	0.07564265335572173
1	36	-0.03213779979281384
2	1	-0.22207769316153758
2	2	-0.19408201003194137
2	3	-0.12593647443602407
2	4	-0.2042850771937168
2	5	-0.10067807140273288
2	6	-0.12772533250657858
2	7	-0.21926070166782097
2	8	-0.14772946396286102
2	9	-0.06213161811025003
2	10	-0.19608225962047499
2	11	-0.11583844440476554
2	12	-0.03438704254950409
2	13	-0.12993012256125525
2	14	-0.10831156833284439
2	15	-0.1406816686330501
2	16	0.0014548522303101663
2	17	-0.24896483681674653
2	18	-0.2620992886974918
2	19	-0.26342110289716913
2	20	-0.29077227304046005
2	21	-0.346896040207163
2	22	-0.39097536985839554
2	23	-0.3723075186599445
2	24	-0.3720875893241171
2	25	-0.3626273521874879
2	26	-0.36507387600037333
2	27	-0.476194466064193
2	28	-0.6002935795266495
2	29	-0.320022667307434
2	30	-0.46637032282806956
2	31	-0.42494435135913733
2	32	-0.2666513400941639
2	33	-0.3707837245530605
2	34	-0.3948520217429099
2	35	-0.4460438951182937
2	36	-0.3776646004640938
3	1	-0.08691779712307124
3	2	-0.06242158715762258
3	3	0.06011068467259406
3	4	0.08027349265727679
3	5	0.08475088096571426
3	6	0.14884279594840422
3	7	-0.03855293873455423
3	8	0.14145802102552807
3	9	0.10809240072443771
3	10	-0.044408545582271586
3	11	0.11675633317950229
3	12	0.10700531462241258
3	13	0.04924317754768737
3	14	0.07779747997602726
3	15	0.030518166363989963
3	16	0.16991078639083668
3	17	-0.10333858745771352
3	18	-0.14157509389564638
3	19	-0.1243543298244063
3	20	-0.2779074411583622
3	21	-0.20761656888517166
3	22	-0.24460456883300452
3	23	-0.26757524106386654
3	24	-0.13923452942802328
3	25	-0.13019339181908407
3	26	-0.1572012250437993
3	27	-0.13148120406221153
3	28	-0.32225086914125
3	29	-0.1408710017267154
3	30	-0.2525840476567609
3	31	-0.2520157726768346
3	32	-0.053877867286793446
3	33	-0.29546021245115384
3	34	-0.1412611417666696
3	35	-0.09573986499951559
3	36	-0.23327831665694632
4	1	-0.150846332104436
4	2	-0.04613277184787279
4	3	0.02148270016783016
4	4	0.045929620922478875
4	5	0.04358523523831792
4	6	-0.014518467649686784
4	7	-0.3424691053663693
4	8	0.10043557705719053
4	9	0.014502412920613494
4	10	-0.03220503953657072
4	11	0.028374087839367235
4	12	0.06541859666252492
4	13	-0.014690713086594087
4	14	0.01865676566079344
4	15	-0.013725096313336849
4	16	0.13231978724774507
4	17	-0.1519577498742919
4	18	-0.22055258314468773
4	19	-0.17669547797621732
4	20	-0.14097200210647287
4	21	-0.11301495224494573
4	22	-0.18054669470321016
4	23	-0.1441668847635782
4	24	-0.08836740408998622
4	25	-0.20006184414860684
4	26	-0.12498736925752496
4	27	-0.1483626916283285
4	28	-0.2896210932971073
4	29	-0.13401346772914735
4	30	-0.2903377563877143
4	31	-0.14606581706640753
4	32	-0.06213116843508004
4	33	-0.37137383518696154
4	34	-0.12282523137558599
4	35	-0.14268343848731746
4	36	-0.3248772798359312
5	1	-0.010457329434579776
5	2	0.03325799573724453
5	3	0.11421057174058546
5	4	0.10799112406168376
5	5	0.13836932966233917
5	6	0.06829151459035643
5	7	-0.09151560630365196
5	8	0.1842791465086222
5	9	0.21007299428205783
5	10	0.0942402856066451
5	11	0.1104107297767456
5	12	0.16219190646646808
5	13	0.10682816197726908
5	14	0.11562254451936127
5	15	0.1197315299230084
5	16	0.2084829728923694
5	17	0.01023969680282022
5	18	-0.024576215849819505
5	19	-0.03301211806467563
5	20	-0.039707975467692336
5	21	-0.17169507654885052
5	22	-0.28197141210728915
5	23	-0.1494628837648193
5	24	-0.22006907198979775
5	25	-0.08558021783047565
5	26	-0.10194850787904386
5	27	-0.10037719244825638
5	28	-0.17332682227497287
5	29	-0.09561561694933118
5	30	-0.30858965181954545
5	31	-0.08533292344223042
5	32	-0.042979043798546
5	33	-0.2806273195068769
5	34	-0.012750661489256743
5	35	-0.024148261174225638
5	36	-0.125919328066324
6	1	-0.06989438989347008
6	2	-0.022253038055119134
6	3	0.1184789041876897
6	4	0.093789632217252
6	5	0.14791196416189933
6	6	0.14975295706657477
6	7	0.03792138145989554
6	8	0.1645815149143246
6	9	0.23519151957161455
6	10	0.12388931420133531
6	11	0.16115058979697494
6	12	0.1398734909256163
6	13	0.15766330178273336
6	14	0.10113539399477389
6	15	0.12579025083871187
6	16	0.24167059599619023
6	17	-0.011298189713208728
6	18	0.010481229198880726
6	19	-0.05338307431194522
6	20	-0.09835773688243421
6	21	-0.21903152988685548
6	22	-0.26550973283980994
6	23	-0.15982473311388645
6	24	-0.14684444738258406
6	25	-0.0695161310633523
6	26	-0.07179200416273801
6	27	-0.03248026483394284
6	28	-0.19366917864471134
6	29	-0.07511953756635847
6	30	-0.14524899184447548
6	31	-0.05559159089497001
6	32	0.011898817825915842
6	33	-0.07043937994451
6	34	0.0087816665964624
6	35	-0.0598108949357794
6	36	-0.11553911357850666
7	1	-0.13338838344245119
7	2	0.0013294524599274382
7	3	0.059602732205419784
7	4	0.08488578990229456
7	5	0.155884367837686
7	6	0.19899137114764187
7	7	0.24162363529624287
7	8	0.24057880779778884
7	9	0.2099929463404706
7	10	0.15583793942930413
7	11	0.17146692717687273
7	12	0.17478097116301683
7	13	0.14983148578935612
7	14	0.14330696795630615
7	15	0.03412144493997715
7	16	0.23191914162402583
7	17	-0.005034084428260144
7	18	-0.02501416584604499
7	19	-0.08050034352298496
7	20	-0.06768581548537611
7	21	-0.10031448717112568
7	22	-0.28432906189512863
7	23	-0.13642280247182725
7	24	-0.0927143865450617
7	25	-0.1227108469311915
7	26	-0.06416442464137617
7	27	-0.03146508138569004
7	28	-0.23353802572908933
7	29	-0.04027448354703722
7	30	-0.10481256056637278
7	31	-0.16820566378927282
7	32	0.0702673370895397
7	33	-0.03511832735289033
7	34	0.06931468842500976
7	35	0.018733384427903843
7	36	0.04513027672013692
8	1	0.15290989286140189
8	2	0.2506370557499906
8	3	0.3522870479068274
8	4	0.336022191344135
8	5	0.39286572921520246
8	6	0.4088350824065614
8	7	0.2277154482169621
8	8	0.39967323240825436
8	9	0.36145239722215905
8	10	0.2763941642632375
8	11	0.35872015171762683
8	12	0.39172249235335954
8	13	0.2683483879905495
8	14	0.32299079254508456
8	15	0.3033680801397196
8	16	0.4645738565219375
8	17	0.2118102575391987
8	18	0.23398964234178976
8	19	0.218069296934857
8	20	0.1781055999117953
8	21	0.08116617494504652
8	22	0.08155824014703938
8	23	0.2041679866603161
8	24	0.1259342964302803
8	25	0.15122991007948627
8	26	0.1404356822870838
8	27	0.23675084536805002
8	28	0.039236360992916275
8	29	0.1808342077108236
8	30	-0.03919746378378619
8	31	0.06604353596724621
8	32	0.2757470051185891
8	33	0.06638290029175664
8	34	0.1761166783491177
8	35	0.20249414481714467
8	36	0.1836006131754857
9	1	0.04090811974625552
9	2	0.08965297684636653
9	3	0.21232177141168052
9	4	0.19908598654933485
9	5	0.25773957946085346
9	6	0.2936934178947226
9	7	0.11035738977804499
9	8	0.2773282876334022
9	9	0.3204121696723803
9	10	0.29179810205644685
9	11	0.24265498973617383
9	12	0.28877099444046905
9	13	0.2477359244686994
9	14	0.2359603910083834
9	15	0.19986248374505067
9	16	0.3469572187915233
9	17	0.14930794879552423
9	18	0.07193430637340725
9	19	-0.007411845246814153
9	20	0.1073656937391334
9	21	-0.04745855262320475
9	22	-0.260394402902719
9	23	-0.1168766736773108
9	24	-0.11698247797775707
9	25	0.03630988496036025
9	26	-0.008616809309089035
9	27	0.060288948873647996
9	28	-0.07217437374167446
9	29	0.0054435682811728725
9	30	-0.2638289882934117
9	31	0.014660136757754572
9	32	0.08916357635717986
9	33	-0.0060931811237807665
9	34	0.04483227806837675
9	35	0.10316275698514232
9	36	-0.10150152564465742
10	1	0.09916885796533847
10	2	0.1296900829685086
10	3	0.23506658822038418
10	4	0.2345071499253919
10	5	0.25806728381424193
10	6	0.29416028071879197
10	7	0.26189919438088793
10	8	0.1021256883804682
10	9	0.32424022523239415
10	10	0.16343667003774698
10	11	0.2012984884374589
10	12	0.21408010492587692
10	13	0.15473920639477257
10	14	0.234100235345565
10	15	0.15012197901026525
10	16	0.31396323392105785
10	17	0.12212416709888174
10	18	0.07067245998896965
10	19	0.060503342543036354
10	20	0.053073958760535334
10	21	-0.08328780286964133
10	22	0.005935716518337131
10	23	-0.028593615038687403
10	24	-0.003589540661309343
10	25	0.032544982184418814
10	26	0.05394758171602376
10	27	0.06557923544295363
10	28	7.824883894613777E-5
10	29	0.018843143126176187
10	30	-0.04646133532008889
10	31	-0.038937944124285195
10	32	0.0842613394325511
10	33	0.0052126156324234785
10	34	0.01961062325025864
10	35	-0.1264524478493243
10	36	-0.05471234718505258
11	1	-6.669149819253121
11	2	-7.0395543609117865
11	3	-7.196381678473504
11	4	-9.520668230114484
11	5	-14.875848197945299
11	6	-19.3868299108979
11	7	-20.082851983515603
11	8	-21.24878859984238
11	9	-22.427247664783966
11	10	-21.35851649740968
11	11	-20.25290667702057
11	12	-18.007128496085485
11	13	-15.542758943091513
11	14	-12.904931914388278
11	15	-11.021665116321135
11	16	-7.586647056781143
11	17	-6.916894653285752
11	18	-6.799112952622973
11	19	-6.785737837566028
11	20	-7.20255980481917
11	21	-4.684961732413374
11	22	-4.682311392583962
11	23	-4.620493663392455
11	24	-4.535172136045169
11	25	-4.5050054592855595
11	26	-4.332987217661852
11	27	-4.191844490436807
11	28	-4.226594035906746
11	29	-4.2297119876505676
11	30	-4.134908307780023
11	31	-4.176397477421958
11	32	-4.090440088024188
11	33	-4.083043533373971
11	34	-3.9765044733280206
11	35	-4.116428939071884
11	36	-4.24917327007531
12	1	-3.0108523432258174
12	2	-3.04000269384769
12	3	-3.0314689123832252
12	4	-3.041594678817102
12	5	-3.142824148847641
12	6	-6.468675316515785
12	7	-7.829929739363163
12	8	-9.605452164117398
12	9	-11.253731215336064
12	10	-14.87088324274135
12	11	-18.272983488514924
12	12	-17.929297157482072
12	13	-17.62507847710787
12	14	-16.896738538846957
12	15	-15.939765186703632
12	16	-13.990179371554223
12	17	-12.736047195209348
12	18	-10.984881187551068
12	19	-9.255379027278895
12	20	-8.437755401716792
12	21	-5.201598571782636
12	22	-5.066649479750858
12	23	-4.972464217088255
12	24	-4.869885791436101
12	25	-4.9468564376911885
12	26	-3.3346515774694723
12	27	-3.2511940911980552
12	28	-3.314257903162172
12	29	-3.1562667648651193
12	30	-3.19098746328212
12	31	-3.204606180119029
12	32	-2.9922579280112487
12	33	-3.04239936644802
12	34	-2.975768169472559
12	35	-3.046514000759011
12	36	-3.2874131493378123
13	1	0.1062342304435333
13	2	0.11759489508038712
13	3	0.17589735677454144
13	4	0.18904478985125905
13	5	0.2682560849172262
13	6	0.2801149507330507
13	7	0.24131997352104406
13	8	0.1722610541139744
13	9	0.3334331853333268
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118	32	0.08810081438655004
118	33	0.10719100947044069
118	34	0.03104283206627656
118	35	0.06538121519531614
118	36	0.040497459167461614
119	1	0.022023109870431767
119	2	0.11901183473046473
119	3	0.18668493655531648
119	4	0.14494186658946973
119	5	0.2305491130570232
119	6	0.1836164776401361
119	7	0.10601247693232096
119	8	0.3019577897062291
119	9	0.2884452720685502
119	10	0.2521627507778845
119	11	0.2776667591660029
119	12	0.23898375362652047
119	13	0.20712337108954415
119	14	0.16467460687331936
119	15	0.16367448345818048
119	16	0.25278111558623806
119	17	0.08318697493859872
119	18	0.05926783001260816
119	19	0.03186878460060427
119	20	-0.0033281039819428315
119	21	0.07074701948020845
119	22	0.008671420203228308
119	23	0.04259244727985845
119	24	-0.05651364268655357
119	25	-0.003821435926653294
119	26	-0.01981380665595367
119	27	-0.09332954209251199
119	28	0.04153142279137256
119	29	-0.05076577816578265
119	30	0.08241257381087763
119	31	0.028477254064647184
119	32	0.009747391712174647
119	33	-0.06400347460590439
119	34	-0.09699559668602475
119	35	0.04414431703566635
119	36	-0.062253739031469024
120	1	-0.03961115435966889
120	2	0.02547173509508127
120	3	0.09065244954349794
120	4	0.04480111615750815
120	5	0.10872611130292142
120	6	0.002789856950499825
120	7	0.17880548968411603
120	8	0.09592026708696721
120	9	0.06806325699781723
120	10	0.13368953440740228
120	11	0.20460720792840448
120	12	0.17482813779346884
120	13	0.13111160379802556
120	14	0.11145168922757875
120	15	0.09391685890702206
120	16	0.23991968345150383
120	17	0.07250191103954506
120	18	0.028836021081275476
120	19	0.05293542634699833
120	20	-0.018696662152912324
120	21	0.04374125226524228
120	22	-0.004566449607395384
120	23	0.03702915398869777
120	24	-0.012900010245722626
120	25	-0.012097904684800653
120	26	-0.06033151563840278
120	27	-0.08883576824614892
120	28	0.005520307687376658
120	29	-0.10516596480521656
120	30	0.014765614830942297
120	31	-0.013503652939007793
120	32	-0.009727807921166232
120	33	-0.23639483273705153
120	34	0.06357769802963276
120	35	-0.006535769617244114
120	36	-0.06663228578745617
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3527139.0
3	24054.0
4	39139.0
5	62521.0
6	39271.0
7	30326.0
8	35176.0
9	35844.0
10	33333.0
11	30642.0
12	40956.0
13	44278.0
14	45273.0
15	42917.0
16	43944.0
17	48393.0
18	53923.0
19	58478.0
20	69969.0
21	90720.0
22	117903.0
23	154248.0
24	189691.0
25	201771.0
26	204596.0
27	196324.0
28	202838.0
29	217003.0
30	237869.0
31	278567.0
32	327989.0
33	406520.0
34	539669.0
35	789437.0
36	1252073.0
37	2290256.0
38	3849553.0
39	1.454042E7
40	748414.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.157119671651575	24.7275259399514	15.088562025958396	32.02679236243863
2	29.93805468275158	24.09651965532547	22.105726534525733	23.859699127397217
3	28.007559281186712	23.135864835193484	21.276432169265092	27.580143714354712
4	30.140808232084034	22.71117429589054	20.42416013837744	26.723857333647988
5	29.700226278194673	23.77494010627477	21.290550725633263	25.234282889897287
6	30.29715126709316	22.412854970932003	19.54162047656844	27.748373285406398
7	29.210466327794048	22.316051480560912	21.842339652821487	26.631142538823553
8	28.824775228044132	20.965669392027106	22.27317822932748	27.93637715060128
9	28.93454799426822	21.081779722417128	22.010021777213858	27.97365050610079
10	29.50735417033953	22.537372532529737	20.443024282012143	27.512249015118595
11	29.984525824635178	21.624301771240315	20.687431103828636	27.703741300295874
12	29.826319909305532	21.366376194968044	20.982696796813393	27.824607098913027
13	28.96710971335545	21.897237083579686	21.253664647276192	27.881988555788674
14	28.946523429285953	21.91760912616589	21.088695063811535	28.047172380736622
15	28.71611860569675	21.542763494974114	21.516836223103834	28.224281676225303
16	29.331290057108006	21.466404651691633	21.031759092620398	28.17054619857996
17	28.92209033111729	21.495905432925124	20.88883218593895	28.69317205001864
18	29.09521852668661	21.466967120148947	20.940377585549083	28.49743676761536
19	29.677056059257247	21.404857671232858	20.903851244397725	28.01423502511217
20	29.424389719814815	21.8261751434134	20.72053758049708	28.0288975562747
21	29.676288861425633	21.518033788926736	20.758694071982443	28.046983277665188
22	29.27839032769443	21.7934541124881	20.878659668886517	28.049495890930952
23	29.23592110032705	21.614812220244616	21.244705470274145	27.90456120915419
24	29.26446415450072	21.524679492314732	20.86694200999588	28.343914343188665
25	29.415512068774767	21.601226075581963	20.70367236969693	28.27958948594634
26	29.443919436766684	21.650065243983153	20.752993413986616	28.153021905263543
27	29.59681978547344	21.862396996247615	20.874875218909708	27.66590799936924
28	29.591673545603335	21.86805911383671	20.693963862869698	27.846303477690252
29	29.564214432478387	21.55863681680354	20.629676138266596	28.247472612451478
30	29.676331911117053	21.929834679984392	20.63237758809246	27.76145582080609
31	30.081457259515926	21.554418245314476	20.67548722055153	27.688637274618067
32	29.348622462749933	21.43189987163655	20.67934003123369	28.540137634379832
33	29.972282060607686	21.597608442422644	20.65901272506288	27.77109677190679
34	30.084308369494263	21.490056504490248	20.836666316242162	27.588968809773323
35	29.184973866959364	21.65870687317641	20.951875912302096	28.204443347562137
36	29.53608919913246	21.82504448963561	21.05392665623287	27.58493965499906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	79590.0
1	57931.0
2	36272.0
3	36272.0
4	31860.5
5	27449.0
6	27449.0
7	30566.0
8	33683.0
9	33683.0
10	40173.0
11	46663.0
12	46663.0
13	55678.0
14	64693.0
15	75385.5
16	86078.0
17	86078.0
18	98744.5
19	111411.0
20	111411.0
21	130967.0
22	150523.0
23	150523.0
24	182121.0
25	213719.0
26	260958.0
27	308197.0
28	308197.0
29	373972.0
30	439747.0
31	439747.0
32	515934.5
33	592122.0
34	592122.0
35	678406.0
36	764690.0
37	764690.0
38	856279.0
39	947868.0
40	1050576.0
41	1153284.0
42	1153284.0
43	1268938.0
44	1384592.0
45	1384592.0
46	1521628.5
47	1658665.0
48	1658665.0
49	1822749.5
50	1986834.0
51	2168796.0
52	2350758.0
53	2350758.0
54	2509086.5
55	2667415.0
56	2667415.0
57	2778550.0
58	2889685.0
59	2889685.0
60	2762569.0
61	2635453.0
62	2635453.0
63	2469528.5
64	2303604.0
65	2120874.0
66	1938144.0
67	1938144.0
68	1770157.0
69	1602170.0
70	1602170.0
71	1457129.5
72	1312089.0
73	1312089.0
74	1184936.5
75	1057784.0
76	940839.0
77	823894.0
78	823894.0
79	716148.0
80	608402.0
81	608402.0
82	509194.5
83	409987.0
84	409987.0
85	327366.0
86	244745.0
87	244745.0
88	186602.5
89	128460.0
90	92161.0
91	55862.0
92	55862.0
93	38047.0
94	20232.0
95	20232.0
96	12980.0
97	5728.0
98	5728.0
99	3336.5
100	945.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.1199900634000928
2	1.3984068879030855
3	1.3435796170870342
4	1.265930663379471
5	1.2747677636070551
6	1.4316134480242513
7	2.6177147830397165
8	1.0796386820556803
9	1.0754962913240003
10	1.5328098057902722
11	1.4169384669050438
12	1.0974573845131168
13	1.3909473734304554
14	1.3042558055365268
15	1.3515400718341932
16	1.2654682569722135
17	1.2855411906650294
18	1.2912859480440801
19	1.351488693344498
20	1.3680711008936421
21	1.4239901646157176
22	1.4315267468228907
23	1.4468246921296535
24	1.3923345926522275
25	1.3141236867136221
26	1.2915396293369508
27	1.0933856392047676
28	1.351401992143137
29	1.1209116650590016
30	1.4537383101492714
31	1.0933952726715854
32	1.1187794577366483
33	1.4315106910448607
34	1.0940375037927763
35	1.1145503658036076
36	1.4721478652382034
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	3.1141437E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.81795055379402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.48014797185205	80.33599111245863
2	6.612289370125708	11.613554019061546
3	1.315092111912663	3.464660821728924
4	0.3156922636334534	1.1089379039191165
5	0.10159462119529619	0.4460915710329976
6	0.043632874998074486	0.22990497954604683
7	0.02654791836993396	0.16319686479019144
8	0.015987193341636778	0.11231700434958429
9	0.01359841713929631	0.10747666115537298
>10	0.06790076155611557	1.1422125037707584
>50	0.005659096536973109	0.3290630065573552
>100	0.0017712880111183439	0.26269635106251166
>500	4.2122879751635704E-5	0.023884095729977566
>1k	2.199430699105318E-5	0.07459749560338344
>5k	1.099707049928766E-5	0.07698236993560614
>10k+	1.0997070492987426E-5	0.5084332392980233
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT	85933	0.27594423468640833	Illumina Paired End PCR Primer 2 (96% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	59668	0.19160323269603777	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4038249423107866E-4	0.0	6.422311211907145E-6	9.633466817860718E-6
2	4.495617848335001E-4	0.0	6.422311211907145E-6	1.284462242381429E-5
3	8.41322768759836E-4	0.0	6.422311211907145E-6	2.568924484762858E-5
4	0.0010821594392063539	0.0	1.284462242381429E-5	2.568924484762858E-5
5	0.001396852688589804	0.0	1.605577802976786E-5	3.5322711665489294E-5
6	0.0016023666473708325	0.0	2.2478089241675008E-5	4.816733408930359E-5
7	0.0018496256290292576	0.0	2.568924484762858E-5	4.816733408930359E-5
8	0.0021418407891710327	0.0	3.211155605953572E-5	5.137848969525716E-5
9	0.0025175459950676007	0.0	3.5322711665489294E-5	7.064542333097859E-5
10	0.00295747431308324	0.0	3.5322711665489294E-5	7.385657893693216E-5
11	0.003724940502906144	0.0	3.853386727144287E-5	7.706773454288574E-5
12	0.0043992831801563945	0.0	4.4956178483350015E-5	8.349004575479289E-5
13	0.005186016303615019	0.0	4.816733408930359E-5	8.670120136074646E-5
14	0.005770446623898569	0.0	5.78008009071643E-5	9.954582378456074E-5
15	0.01962337190798228	0.0	5.78008009071643E-5	1.1239044620837503E-4
16	0.02077938792612557	3.2111556059535724E-6	5.78008009071643E-5	1.156016018143286E-4
17	0.02244597768561547	6.422311211907145E-6	5.78008009071643E-5	1.3807969105600362E-4
18	0.023968065442837465	6.422311211907145E-6	6.101195651311787E-5	1.4129084666195717E-4
19	0.026716814641533724	6.422311211907145E-6	6.101195651311787E-5	1.541354690857715E-4
20	0.3244776405147906	6.422311211907145E-6	6.101195651311787E-5	1.6055778029767863E-4
21	0.3304728680311059	6.422311211907145E-6	7.385657893693216E-5	1.637689359036322E-4
22	0.33250552952967455	6.422311211907145E-6	7.706773454288574E-5	1.7019124711553933E-4
23	0.33544052575351613	6.422311211907145E-6	7.706773454288574E-5	1.7661355832744648E-4
24	0.3394416256385343	6.422311211907145E-6	7.706773454288574E-5	1.8303586953935362E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCCGA	14900	0.0	22.457808	14
CGGTTCA	15120	0.0	22.099941	1
AATGCCG	15300	0.0	22.048594	13
AGCGGTT	13870	0.0	20.850561	30
TGCCGAG	17480	0.0	19.201363	15
ATCGGAA	15355	0.0	19.119833	22
CTCGTAT	2230	0.0	19.03148	28
GAATGCC	17925	0.0	18.94877	12
TCTCGTA	2260	0.0	18.91638	27
GTTCAGC	17790	0.0	18.808695	3
GAGCGGT	15635	0.0	18.657469	29
AAGAGCG	15765	0.0	18.497108	27
GACCGAT	2465	0.0	18.220606	21
TCGGAAG	16285	0.0	18.179941	23
CGGAAGA	16445	0.0	17.913391	24
GATCGGA	16620	0.0	17.727705	21
ACCGATC	2480	0.0	17.69676	22
TCGTATG	2430	0.0	17.581112	29
GGTTCAG	19240	0.0	17.446629	2
TTCAGCA	19580	0.0	17.12143	4
>>END_MODULE
