FastQCFastQC Report
Sun 14 Aug 2016
V400_H3K27ac_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV400_H3K27ac_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31141437
Sequences flagged as poor quality0
Sequence length36
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT859330.27594423468640833Illumina Paired End PCR Primer 2 (96% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN596680.19160323269603777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA149000.022.45780814
CGGTTCA151200.022.0999411
AATGCCG153000.022.04859413
AGCGGTT138700.020.85056130
TGCCGAG174800.019.20136315
ATCGGAA153550.019.11983322
CTCGTAT22300.019.0314828
GAATGCC179250.018.9487712
TCTCGTA22600.018.9163827
GTTCAGC177900.018.8086953
GAGCGGT156350.018.65746929
AAGAGCG157650.018.49710827
GACCGAT24650.018.22060621
TCGGAAG162850.018.17994123
CGGAAGA164450.017.91339124
GATCGGA166200.017.72770521
ACCGATC24800.017.6967622
TCGTATG24300.017.58111229
GGTTCAG192400.017.4466292
TTCAGCA195800.017.121434
Produced by FastQC (version 0.11.3)