##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	Crypt4_H3K4me3_ChIPSeq.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	47234860
Sequences flagged as poor quality	0
Sequence length	36
%GC	68
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.559791666578455	36.0	29.0	38.0	2.0	40.0
2	28.657129924805535	36.0	24.0	39.0	2.0	40.0
3	28.564706130175892	36.0	24.0	39.0	2.0	40.0
4	28.443822041602324	36.0	23.0	39.0	2.0	40.0
5	26.622312927359157	33.0	15.0	39.0	2.0	40.0
6	26.72199142751773	34.0	14.0	39.0	2.0	40.0
7	26.55516518943848	34.0	13.0	39.0	2.0	40.0
8	26.251453418089945	33.0	12.0	39.0	2.0	40.0
9	26.494478400063006	33.0	13.0	39.0	2.0	40.0
10	26.338642773578666	33.0	12.0	39.0	2.0	40.0
11	27.95459766367467	36.0	17.0	39.0	2.0	40.0
12	27.9166122224137	36.0	17.0	39.0	2.0	40.0
13	27.879082990824998	36.0	17.0	39.0	2.0	40.0
14	27.815190242968857	36.0	16.0	39.0	2.0	40.0
15	27.5626872398902	35.0	14.0	39.0	2.0	40.0
16	27.62014018883511	36.0	9.0	39.0	2.0	40.0
17	27.474117547929644	35.0	2.0	39.0	2.0	40.0
18	27.35456084764515	35.0	2.0	39.0	2.0	40.0
19	27.242924568845975	35.0	2.0	39.0	2.0	40.0
20	27.047884189770013	35.0	2.0	39.0	2.0	40.0
21	26.885650216810212	35.0	2.0	39.0	2.0	40.0
22	26.617599290015892	35.0	2.0	39.0	2.0	40.0
23	26.58911520008739	35.0	2.0	39.0	2.0	40.0
24	26.37312546284672	35.0	2.0	39.0	2.0	40.0
25	26.130781122247424	34.0	2.0	39.0	2.0	40.0
26	25.471919446781467	33.0	2.0	38.0	2.0	40.0
27	25.171184946880334	33.0	2.0	38.0	2.0	40.0
28	24.926624361753163	33.0	2.0	38.0	2.0	40.0
29	24.719916095866484	33.0	2.0	38.0	2.0	39.0
30	24.450004805772686	32.0	2.0	37.0	2.0	39.0
31	24.335075577655992	33.0	2.0	37.0	2.0	39.0
32	24.30681153707241	32.0	2.0	37.0	2.0	39.0
33	24.312458065928425	33.0	2.0	38.0	2.0	39.0
34	24.20865672090486	32.0	2.0	38.0	2.0	39.0
35	24.045354723185376	32.0	2.0	37.0	2.0	39.0
36	23.962798111394847	32.0	2.0	38.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	0.6910351124859311
1	2	0.907673205485299
1	3	0.9052330389518346
1	4	1.134896338003152
1	5	2.9767917518454965
1	6	2.796541385302639
1	7	2.739863045225089
1	8	2.8468313699929837
1	9	2.7683712285802393
1	10	2.838562247108097
1	11	1.276941344855416
1	12	1.3160694540325188
1	13	1.2417476510077776
1	14	1.29144205740144
1	15	1.4439659329394203
1	16	1.4759689358887016
1	17	1.4681688771353798
1	18	1.51063333973444
1	19	1.5549063766038422
1	20	1.616247142090124
1	21	1.6486771444253172
1	22	1.8089038488006501
1	23	1.7257841686282305
1	24	1.738736560115722
1	25	1.9453756225252086
1	26	2.125986224990381
1	27	2.326136149114948
1	28	2.639176943625131
1	29	2.524409121387354
1	30	2.663917478196691
1	31	2.614496765232026
1	32	2.580193746211492
1	33	2.4721055634173403
1	34	2.5392069369876715
1	35	2.5026942998454906
1	36	2.5978038088107347
2	1	0.28916890567036546
2	2	0.6971260947674054
2	3	0.6045021596033671
2	4	0.8344847906162052
2	5	2.76526348921794
2	6	2.595939533821465
2	7	2.607392731709659
2	8	2.7698766074781886
2	9	2.607642134057219
2	10	2.659514865163459
2	11	1.1950735283608296
2	12	1.154305050657964
2	13	1.1584329082161346
2	14	1.1340307165204635
2	15	1.3291018081978017
2	16	1.2947734664762756
2	17	1.3636261677636803
2	18	1.416392619492317
2	19	1.4915611666750088
2	20	1.5521822455032428
2	21	1.5773846255462018
2	22	1.7892005935459245
2	23	1.7514778235237216
2	24	1.7868102080187
2	25	1.8565889385240517
2	26	2.134269096137327
2	27	2.1450734089975434
2	28	2.313383441924376
2	29	2.328630392036281
2	30	2.3481654611771283
2	31	2.500443066339752
2	32	2.5354881540030725
2	33	2.455132138634223
2	34	2.381881487912242
2	35	2.442600965151019
2	36	2.479998485226883
3	1	0.34950748673998433
3	2	0.8431842682007016
3	3	0.8933049389114842
3	4	1.0317272007303657
3	5	2.9360103370546895
3	6	2.8266073869512063
3	7	2.802112179794424
3	8	2.945134208641498
3	9	2.8374188484297846
3	10	2.8908910892059865
3	11	1.4274774577385188
3	12	1.445350210249508
3	13	1.3725346242694094
3	14	1.4448778480257118
3	15	1.6164826616799814
3	16	1.6001754240421207
3	17	1.664008948893656
3	18	1.6884984793990192
3	19	1.7288365921413806
3	20	1.8619285863629464
3	21	1.8638204448569056
3	22	2.0585100854251266
3	23	1.9335860538828022
3	24	1.9925322148532878
3	25	2.1550321941286654
3	26	2.512200857521684
3	27	2.433010005508134
3	28	2.597488862082333
3	29	2.727146118147026
3	30	2.544482636363309
3	31	2.9278153485775427
3	32	2.8833062664565183
3	33	2.7917384769968727
3	34	2.7478045306447036
3	35	2.7599866217459557
3	36	2.749910821779899
4	1	0.6859006121165585
4	2	0.9846486661322693
4	3	0.946099773196174
4	4	1.234350364384305
4	5	3.095824944176499
4	6	2.9621276019312432
4	7	2.924388595994362
4	8	3.1377380717640584
4	9	2.9495261799096966
4	10	2.983864676868361
4	11	1.5543265218448248
4	12	1.451628740790106
4	13	1.5518699514147052
4	14	1.581471289523435
4	15	1.7113468792966913
4	16	1.620654733445786
4	17	1.7214996664673272
4	18	1.708124695751831
4	19	1.7746513158682689
4	20	1.8546692765325865
4	21	1.925959796753375
4	22	2.063337452894441
4	23	1.938659064478781
4	24	2.025147179367824
4	25	2.0773703613616803
4	26	2.3518085586801547
4	27	2.3323681626034976
4	28	2.394775191553105
4	29	2.7479169310499465
4	30	2.385245941664831
4	31	2.3035089280885934
4	32	2.5963933932558945
4	33	2.584855319699198
4	34	2.6214512128440006
4	35	2.585680100101186
4	36	2.6530746817096116
5	1	0.7002305427689883
5	2	1.0182449644298828
5	3	1.1028952634154585
5	4	1.257996145869864
5	5	3.1344780058713084
5	6	2.980044287802574
5	7	2.962621726904718
5	8	3.2195351022169056
5	9	3.003626000825701
5	10	2.999408945612945
5	11	1.5245501764971294
5	12	1.4177056118785814
5	13	1.519048063445041
5	14	1.5576638095702116
5	15	1.7043268160753158
5	16	1.6705882254656395
5	17	1.7554208932483064
5	18	1.7155711517902077
5	19	1.7679872666336038
5	20	1.8784916058428607
5	21	1.9320421964696912
5	22	1.9832782633398658
5	23	1.9946851067015636
5	24	2.0274811717203924
5	25	2.098421665731056
5	26	2.417344205590993
5	27	2.2501896348259045
5	28	2.415203095072876
5	29	2.3402037656198296
5	30	2.4795234007287803
5	31	2.4671415122044564
5	32	2.562528118643833
5	33	2.576188138810693
5	34	2.5931701853020783
5	35	2.50158053048645
5	36	2.427183859254999
6	1	0.49460259484445857
6	2	0.8699777468347349
6	3	0.7432366667009944
6	4	0.9871659451088952
6	5	2.916724526399637
6	6	2.877630925065354
6	7	2.7024539671954244
6	8	2.915277268820301
6	9	2.8397368941842807
6	10	2.8430109784715967
6	11	1.4489463035830283
6	12	1.3841199565746578
6	13	1.4549630801503426
6	14	1.4127201068606539
6	15	1.6027608678937177
6	16	1.5638911725289368
6	17	1.6586277630780408
6	18	1.6889227525255066
6	19	1.7265158606581963
6	20	1.7863367716157086
6	21	1.7451220243780234
6	22	1.9800864313785524
6	23	1.933268483450604
6	24	1.9485059981971702
6	25	2.058671744526368
6	26	2.17112257658
6	27	2.006804927232679
6	28	2.4612003111965883
6	29	2.3749790005891924
6	30	2.5111218623737663
6	31	2.52235090563709
6	32	2.4041977317340653
6	33	2.4202067898627355
6	34	2.4101368585991665
6	35	2.2833148309528433
6	36	2.445001184071124
7	1	0.5230139848260329
7	2	0.8129587343532947
7	3	0.8424812424472279
7	4	1.0132958929843134
7	5	2.9870085789033247
7	6	2.8757899614933145
7	7	2.841830552557443
7	8	3.0311850504502615
7	9	2.8551239726113558
7	10	2.9689148980995768
7	11	1.5189661040531242
7	12	1.5265503106024205
7	13	1.5232766512488283
7	14	1.5224856204665578
7	15	1.6967908732486485
7	16	1.7094102326558733
7	17	1.7378332301163546
7	18	1.7640696773189362
7	19	1.8654918436633494
7	20	1.9322425972290347
7	21	1.947453122212977
7	22	2.0439033875709676
7	23	1.9228615484085196
7	24	2.05871498867036
7	25	2.2468772127897694
7	26	2.4476819142957886
7	27	2.4928056062854402
7	28	2.6866216909213705
7	29	2.8213231621924884
7	30	2.655357878085539
7	31	2.6800317875253654
7	32	2.921283583275933
7	33	2.828469548924879
7	34	2.7688677685777954
7	35	2.795868133073572
7	36	2.9056917425882567
8	1	0.5221291984005525
8	2	0.9062340286455495
8	3	0.9901263626701535
8	4	1.097790736869392
8	5	2.9483707625419235
8	6	2.786139699955985
8	7	2.73315890203083
8	8	3.0092104676747837
8	9	2.8659826046216352
8	10	2.8108386964253143
8	11	1.4396321740884659
8	12	1.3333132613641077
8	13	1.411597037709658
8	14	1.398571779814489
8	15	1.590776179403548
8	16	1.5676028969413913
8	17	1.6235197641609496
8	18	1.6298447396635716
8	19	1.6722165751255815
8	20	1.8184858767727583
8	21	1.8777690327650944
8	22	2.0180620940911105
8	23	1.929500007212578
8	24	2.048233500826125
8	25	2.089388520762178
8	26	2.4109445403604752
8	27	2.21362374219299
8	28	2.5192523560598907
8	29	2.6151571526784068
8	30	2.579937272662569
8	31	2.4269175727240118
8	32	2.6274170893246165
8	33	2.556718474428486
8	34	2.5652585491952458
8	35	2.5796785985229
8	36	2.535187124866688
9	1	0.4720512253822946
9	2	0.9552903889308446
9	3	0.9865541514145875
9	4	1.0898104611597041
9	5	2.941665794144548
9	6	2.787218553603001
9	7	2.851067676663323
9	8	2.9168590902488702
9	9	2.8652591358131794
9	10	2.904742362851014
9	11	1.4779698025726837
9	12	1.3624374362869673
9	13	1.4604234067909232
9	14	1.4833815478379364
9	15	1.5879627785184773
9	16	1.664136354040064
9	17	1.7047252904204733
9	18	1.7595331364429967
9	19	1.8146529627515768
9	20	1.885332857109539
9	21	1.937387116379405
9	22	2.1091146719448446
9	23	2.0171122726638693
9	24	2.102830547456289
9	25	2.2946240051277194
9	26	2.4207217061790907
9	27	2.2593363879201647
9	28	2.7119494347447173
9	29	2.634079233485643
9	30	2.8235030996436556
9	31	2.580925322468204
9	32	2.603049801224202
9	33	2.6809163396928177
9	34	2.638153911570466
9	35	2.6888661436259085
9	36	2.710216602980246
10	1	0.53029571932667
10	2	0.9264708979029201
10	3	1.0907199330202815
10	4	1.199034837237221
10	5	3.003642137890104
10	6	2.9581726430644473
10	7	2.9765863097371863
10	8	3.073122410913747
10	9	2.9867871636601073
10	10	2.9622213818131478
10	11	1.5972007973513236
10	12	1.5515774211226194
10	13	1.55858811819909
10	14	1.5234299596184684
10	15	1.7652593095399212
10	16	1.6992823121362584
10	17	1.7897399677870922
10	18	1.8052318329268857
10	19	1.9118343470088774
10	20	1.9726757908803627
10	21	1.9868287203516921
10	22	2.1514000797056063
10	23	2.0536810170523054
10	24	2.08310260646045
10	25	1.984878800952803
10	26	2.5597649966719764
10	27	2.2804254363727594
10	28	2.6790295674239424
10	29	2.8666024578833706
10	30	2.9175015147426393
10	31	2.8782739365404666
10	32	2.8162640671269656
10	33	2.7182935645287785
10	34	2.785052039551694
10	35	2.7393891884439476
10	36	2.8314779541509125
11	1	0.5228949626036474
11	2	0.8120530948282934
11	3	0.9761516188697108
11	4	1.0510599135549867
11	5	2.902246662811443
11	6	2.7376994566735746
11	7	2.6951710116590846
11	8	2.7894332085655655
11	9	2.617195912387757
11	10	2.7921418489280185
11	11	1.3642434050459755
11	12	1.3434363623512091
11	13	1.3544790787581071
11	14	1.3534246229473084
11	15	1.460399342885701
11	16	1.4131824076089217
11	17	1.5474470142594328
11	18	1.5397621472390526
11	19	1.576574219407064
11	20	1.5562158665294454
11	21	1.7040384342308919
11	22	1.7824395760640854
11	23	1.801122415828992
11	24	1.8515599631260784
11	25	1.6210340776517143
11	26	2.0696164922168947
11	27	2.214758231743634
11	28	1.9987094043031384
11	29	2.2759452921630725
11	30	2.2416558056027114
11	31	2.3088408350859027
11	32	2.395225512260833
11	33	2.3035318526426174
11	34	2.2359727169060974
11	35	2.3258509812682675
11	36	2.428956673057286
12	1	0.45606922789937343
12	2	0.8756496645410081
12	3	0.9383372383086481
12	4	1.0443895643505066
12	5	2.833993752613889
12	6	2.7629646026006256
12	7	2.665438495378222
12	8	2.7971331633114893
12	9	2.7387274257726375
12	10	2.786335719529802
12	11	1.3191185227112285
12	12	1.3719162145585244
12	13	1.3618933005278855
12	14	1.4411102778256577
12	15	1.5511215510904428
12	16	1.5579898479448602
12	17	1.612682019426913
12	18	1.6086284319845348
12	19	1.641475889464143
12	20	1.7630916266632823
12	21	1.82440137749008
12	22	1.8814603016225746
12	23	1.8916694498547848
12	24	1.9594925241424583
12	25	1.9923576958526503
12	26	2.2815994056580386
12	27	1.9236651540436682
12	28	2.497792946689664
12	29	2.508027201729387
12	30	2.542475586675426
12	31	2.4195931525145298
12	32	2.5990347838368955
12	33	2.6247877721748516
12	34	2.4846287359017367
12	35	2.518290613080616
12	36	2.601515646027547
13	1	0.5218783275004242
13	2	0.7779585601260415
13	3	0.9163111494219507
13	4	0.9676305637911291
13	5	2.695914890114988
13	6	2.6081759623666834
13	7	2.5055045868326857
13	8	2.6745689386747493
13	9	2.5401857339081815
13	10	2.662494230652783
13	11	1.3069384919623452
13	12	1.3181184410377007
13	13	1.2828416375011784
13	14	1.2384633539215635
13	15	1.4244702634568824
13	16	1.366562969204395
13	17	1.4348860683205942
13	18	1.4357107054034657
13	19	1.3834289917703444
13	20	1.5672306933104423
13	21	1.6084555334336201
13	22	1.680102913225948
13	23	1.6697607966828407
13	24	1.7122030865332931
13	25	1.64443678114824
13	26	2.0016716924401763
13	27	1.8373051166072116
13	28	1.63710957614855
13	29	1.7335936513024421
13	30	2.226087462712666
13	31	2.242034021620004
13	32	2.080953878881907
13	33	2.1237905778886947
13	34	1.9986106170019582
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118	5	2.747156096164108
118	6	2.6989699358958674
118	7	2.6530537936655847
118	8	2.9283749156616317
118	9	2.7028495207607577
118	10	2.7005695080047545
118	11	1.2892144681977022
118	12	1.3605174167682677
118	13	1.271259174168879
118	14	1.328743679389973
118	15	1.4344355972486795
118	16	1.4446115314077979
118	17	1.467092403293897
118	18	1.4569804659485683
118	19	1.503589660139781
118	20	1.5419103397302187
118	21	1.6253039180164564
118	22	1.7607577655170843
118	23	1.7501190095921082
118	24	1.860514431772284
118	25	1.9915231827773
118	26	2.244278123224266
118	27	2.4154429489294174
118	28	2.4396984267520168
118	29	2.526343578637924
118	30	2.7051782794618475
118	31	2.7572274567969046
118	32	2.5639620698116623
118	33	2.463579334328557
118	34	2.472380616902438
118	35	2.521981281286209
118	36	2.5427771450188352
119	1	0.49557462916902395
119	2	0.6563646247853825
119	3	0.8317708679626854
119	4	0.9986297920391998
119	5	2.78784188528536
119	6	2.6889429302501924
119	7	2.674331671311286
119	8	2.8644946311877355
119	9	2.6469509571288654
119	10	2.6789577902638726
119	11	1.3193350368359873
119	12	1.260190724235052
119	13	1.1925230624777292
119	14	1.2265716568202514
119	15	1.3201241525604601
119	16	1.336506141229556
119	17	1.359640512641061
119	18	1.3969964559101022
119	19	1.3883145658830252
119	20	1.4555863242193467
119	21	1.5963590669371328
119	22	1.7091141806226808
119	23	1.660694748052716
119	24	1.770612709461151
119	25	1.9212191584435416
119	26	2.2049887357526288
119	27	2.1493264489826025
119	28	2.4719684779884155
119	29	2.4967089369345636
119	30	2.532542028000659
119	31	2.612994861843088
119	32	2.424713730455167
119	33	2.360709637825451
119	34	2.369338509699638
119	35	2.3687999117175416
119	36	2.4295184188352543
120	1	0.33158618558922015
120	2	0.5130656707486487
120	3	0.503710755039041
120	4	0.7922048266456407
120	5	2.5910460534839963
120	6	2.4672343138380306
120	7	2.461446003955473
120	8	2.6436710855994683
120	9	2.4840825851222874
120	10	2.519423442841191
120	11	1.1414669128941561
120	12	1.0816873724693679
120	13	1.0327737691059866
120	14	1.0796810738551699
120	15	1.214515974109343
120	16	1.181678124336365
120	17	1.21785125090992
120	18	1.245294856440978
120	19	1.3041429503909399
120	20	1.3768181362015781
120	21	1.4248404352695694
120	22	1.518148444139161
120	23	1.534363568739984
120	24	1.5951266026032123
120	25	1.721725667052695
120	26	1.9818646226628118
120	27	2.2998535128299267
120	28	2.470548376702105
120	29	2.447244820171335
120	30	2.406642729081014
120	31	2.6354529768065333
120	32	2.4877871813330756
120	33	2.38983391288647
120	34	2.343227061359112
120	35	2.3486977842317494
120	36	2.423177632440513
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	9355815.0
3	267965.0
4	455490.0
5	700624.0
6	155164.0
7	106990.0
8	124688.0
9	157973.0
10	175112.0
11	164232.0
12	180137.0
13	197870.0
14	208331.0
15	210315.0
16	213174.0
17	221732.0
18	228973.0
19	234775.0
20	251670.0
21	288570.0
22	336099.0
23	384147.0
24	435670.0
25	492324.0
26	561904.0
27	642822.0
28	735969.0
29	852515.0
30	1001472.0
31	1188423.0
32	1439263.0
33	1757865.0
34	2157804.0
35	2663141.0
36	3293219.0
37	4036057.0
38	4537390.0
39	6799516.0
40	19660.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.26826326405677	19.136136605402513	10.473742558182146	35.12185757235857
2	36.65808516649248	21.19762049617716	14.51831936027666	27.6259749770537
3	34.39739466829445	17.55953427184197	17.123104733649434	30.919966326214137
4	34.893091063414325	17.211546424467095	15.512918724327546	32.38244378779104
5	36.387804197792	18.24759939184522	16.387983998585568	28.976612411777207
6	36.40938226613559	16.156623987536868	13.19768451330178	34.236309233025764
7	33.2039902208767	20.705139084047197	14.696452182284295	31.394418512791816
8	35.47622914666408	18.032045105166976	14.663437252222177	31.828288495946765
9	35.781753121337914	15.150126403438072	14.264050987026547	34.80406948819746
10	33.74829598261799	20.330750752094055	13.816741919193237	32.10421134609472
11	38.97979342239562	15.48746178478401	13.743260880820362	31.789483912000005
12	35.875183135537114	15.18605526587887	14.365313690712583	34.57344790787143
13	36.03723070148333	17.391616816300623	14.445674171981853	32.12547831023419
14	35.83471691718314	17.414041471629357	14.075319657164625	32.67592195402288
15	33.16910430633853	15.630586830086276	18.825579659886042	32.37472920368914
16	35.87224519472293	15.234568997663636	16.372748366497373	32.520437441116066
17	33.29762228557637	15.395851391554258	13.930844859682088	37.37568146318729
18	33.34699095473534	17.907576240291302	14.072379659968014	34.67305314500535
19	38.439901257953885	15.098608550786672	13.823484258932336	32.638005932327104
20	34.05442703026238	17.216699856489452	13.737259627856771	34.9916134853914
21	35.64648991560359	17.96944753200338	13.633939312101068	32.75012324029197
22	36.028261243977475	17.60896285948977	13.675808166065162	32.68696773046759
23	35.90505879433226	15.368671610990495	15.863858830675726	32.862410764001524
24	34.019673736869066	17.559351963923483	13.726425190008293	34.69454910919916
25	36.01314963681657	17.709989221465428	13.58298305038495	32.69387809133305
26	36.333290631097306	15.309753351242586	16.075698756808414	32.28125726085169
27	37.38086009313983	16.943472596202287	13.714494966493234	31.961172344164652
28	34.874097447410314	17.1663401052048	13.513913723414772	34.445648723970116
29	37.072314924954355	14.984999075881802	13.712330967073724	34.23035503209012
30	37.42353725137189	17.01133207779313	13.615030745752083	31.950099925082892
31	36.82546758747618	17.434401637221953	13.764103619421656	31.97602715588021
32	36.86911672458171	15.003084502813891	13.727846153849441	34.39995261875496
33	36.33328653112485	17.698637560079806	13.56667268002407	32.40140322877127
34	36.45924028738137	14.897051842954125	15.371235325543589	33.27247254412092
35	34.28418038891272	15.148986242190166	15.41645903921061	35.1503743296865
36	36.615813364038765	15.338921335111952	15.294868153610535	32.75039714723875
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108269.0
1	97299.5
2	86330.0
3	86330.0
4	60252.0
5	34174.0
6	34174.0
7	31446.5
8	28719.0
9	28719.0
10	30386.5
11	32054.0
12	32054.0
13	36112.5
14	40171.0
15	46057.0
16	51943.0
17	51943.0
18	60538.5
19	69134.0
20	69134.0
21	83922.0
22	98710.0
23	98710.0
24	123046.5
25	147383.0
26	183690.5
27	219998.0
28	219998.0
29	272860.0
30	325722.0
31	325722.0
32	376151.0
33	426580.0
34	426580.0
35	476090.5
36	525601.0
37	525601.0
38	562093.5
39	598586.0
40	619578.0
41	640570.0
42	640570.0
43	657140.0
44	673710.0
45	673710.0
46	700212.0
47	726714.0
48	726714.0
49	776358.0
50	826002.0
51	908227.5
52	990453.0
53	990453.0
54	1134890.5
55	1279328.0
56	1279328.0
57	2317895.5
58	3356463.0
59	3356463.0
60	2900585.5
61	2444708.0
62	2444708.0
63	2523372.5
64	2602037.0
65	2926202.5
66	3250368.0
67	3250368.0
68	3575633.0
69	3900898.0
70	3900898.0
71	4160964.0
72	4421030.0
73	4421030.0
74	4537490.0
75	4653950.0
76	4553880.5
77	4453811.0
78	4453811.0
79	4135757.0
80	3817703.0
81	3817703.0
82	3346328.0
83	2874953.0
84	2874953.0
85	2362641.5
86	1850330.0
87	1850330.0
88	1434129.5
89	1017929.0
90	734754.5
91	451580.0
92	451580.0
93	306036.5
94	160493.0
95	160493.0
96	101310.5
97	42128.0
98	42128.0
99	24228.0
100	6328.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8380124340370649
2	0.9932156038993235
3	0.871165914326834
4	0.8878252206103712
5	1.0934508962236789
6	0.9794948053196304
7	0.9515853333745458
8	1.1561524687487164
9	0.9429243571379274
10	0.9896038646033882
11	1.166305986722518
12	1.0390461620929965
13	0.9916468472649225
14	1.0074296822304545
15	1.131854736099567
16	1.0718460899428939
17	1.0378394262203805
18	1.0410277494206608
19	1.0408668513043122
20	1.050357723088414
21	1.0390906207830404
22	1.0969906547833528
23	1.0880629264064718
24	1.0246796539674299
25	1.0041926661791736
26	1.0924029413869334
27	0.9539098877396907
28	0.9211290136140977
29	0.9062734598980499
30	0.901435931005194
31	0.9087208049309344
32	0.913585855870008
33	0.9060088248382656
34	0.9027167646945498
35	0.8939351148706696
36	0.8912654763875663
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	4.723486E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.6224210665788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.51756235912707	49.169598511746145
2	9.825650194274884	12.306120074375873
3	4.838756749035277	9.090439877305114
4	2.714675841223603	6.799982943534939
5	1.6265907696021233	5.093052603851731
6	0.980889595067391	3.685540874528169
7	0.5744896473141862	2.5183152814749525
8	0.3491000843868046	1.7489193983078937
9	0.2025209707212975	1.1414118152989232
>10	0.36207047615484844	2.952642345132168
>50	0.005025933548572817	0.21836293470863105
>100	0.00228904133503971	0.2724480414814815
>500	2.0086289360067572E-4	0.08624408985073115
>1k	1.1669610395284227E-4	0.1340441874353969
>5k	1.3506497341996717E-5	0.063249632112892
>10k+	4.727271401783442E-5	4.71962738885501
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT	793840	1.680623166872941	Illumina Paired End PCR Primer 2 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCG	703378	1.4891078326473286	Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG	311941	0.660404201473234	Illumina Paired End PCR Primer 2 (100% over 36bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAA	94129	0.19927866833944252	Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC	68860	0.14578216173393974	Illumina Paired End PCR Primer 2 (100% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	67906	0.14376246695766642	No Hit
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCA	57950	0.12268481371597163	Illumina Paired End PCR Primer 2 (100% over 22bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001619566565879522	2.117080478273885E-6	2.117080478273885E-6	1.693664382619108E-5
2	0.002303383560361987	2.117080478273885E-6	2.117080478273885E-6	2.5404965739286622E-5
3	0.0059553473853844385	6.3512414348216554E-6	2.117080478273885E-6	4.23416095654777E-5
4	0.007418249995871693	6.3512414348216554E-6	2.117080478273885E-6	5.7161172913394896E-5
5	0.010132347169018814	6.3512414348216554E-6	8.46832191309554E-6	6.351241434821656E-5
6	0.011853533597855482	6.3512414348216554E-6	8.46832191309554E-6	7.409781673958598E-5
7	0.013746203545432335	6.3512414348216554E-6	1.2702482869643311E-5	8.044905817440763E-5
8	0.015630405171096092	8.46832191309554E-6	1.2702482869643311E-5	9.738570200059871E-5
9	0.018397429356200062	1.0585402391369424E-5	1.2702482869643311E-5	1.1432234582678979E-4
10	0.021911782950134708	1.0585402391369424E-5	1.2702482869643311E-5	1.270248286964331E-4
11	0.02669638483103369	1.0585402391369424E-5	1.2702482869643311E-5	1.3761023108780252E-4
12	0.03216056954545859	1.2702482869643311E-5	1.4819563347917196E-5	1.6301519682708916E-4
13	0.03644765751396321	1.2702482869643311E-5	1.693664382619108E-5	1.672493577836369E-4
14	0.03975030306007046	1.2702482869643311E-5	2.117080478273885E-5	1.8842016256637577E-4
15	0.18176406154268268	1.4819563347917196E-5	2.117080478273885E-5	1.9053724304464965E-4
16	0.18823385948428767	1.4819563347917196E-5	2.117080478273885E-5	1.9477140400119743E-4
17	0.2020456925245465	1.4819563347917196E-5	2.117080478273885E-5	2.095909673491146E-4
18	0.21032771135555392	1.4819563347917196E-5	2.117080478273885E-5	2.1805928926221015E-4
19	0.22436607200698805	1.4819563347917196E-5	2.117080478273885E-5	2.265276111753057E-4
20	2.090498415788678	1.4819563347917196E-5	2.117080478273885E-5	2.3287885261012736E-4
21	2.1408362383205963	1.693664382619108E-5	2.117080478273885E-5	2.4346425500149676E-4
22	2.150125987459262	2.117080478273885E-5	2.117080478273885E-5	2.646350597842356E-4
23	2.1591024086871435	2.3287885261012735E-5	2.3287885261012735E-5	2.9004002552352224E-4
24	2.1682947721238084	2.3287885261012735E-5	2.5404965739286622E-5	3.0485958887143945E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAT	9585	0.0	26.00298	28
TCGTATG	9740	0.0	25.958057	29
TCTCGTA	9870	0.0	25.01698	27
ATCTCGT	9985	0.0	24.572676	26
GCGGTTC	149375	0.0	22.90723	11
AGATCGG	124910	0.0	21.638416	20
CCGATCT	11675	0.0	21.045963	23
GACCGAT	12525	0.0	20.053087	21
ACCGATC	12560	0.0	19.675833	22
GTATGCC	5580	0.0	19.196606	2
TATGCCG	5720	0.0	18.894749	3
CGTATGC	13685	0.0	18.511902	30
GAGACCG	60990	0.0	18.285185	30
CGAGACC	61285	0.0	18.146856	29
CCGAGAC	62430	0.0	17.767956	28
CGATCTC	14340	0.0	17.511261	24
GATCTCG	14665	0.0	17.252104	25
CTTGAAA	6835	0.0	17.133003	17
ATGCCGT	6690	0.0	16.850845	4
AGACCGA	16160	0.0	16.015835	20
>>END_MODULE
