FastQCFastQC Report
Sun 14 Aug 2016
Crypt4_H3K4me3_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCrypt4_H3K4me3_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47234860
Sequences flagged as poor quality0
Sequence length36
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT7938401.680623166872941Illumina Paired End PCR Primer 2 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCG7033781.4891078326473286Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG3119410.660404201473234Illumina Paired End PCR Primer 2 (100% over 36bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAA941290.19927866833944252Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC688600.14578216173393974Illumina Paired End PCR Primer 2 (100% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN679060.14376246695766642No Hit
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCA579500.12268481371597163Illumina Paired End PCR Primer 2 (100% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT95850.026.0029828
TCGTATG97400.025.95805729
TCTCGTA98700.025.0169827
ATCTCGT99850.024.57267626
GCGGTTC1493750.022.9072311
AGATCGG1249100.021.63841620
CCGATCT116750.021.04596323
GACCGAT125250.020.05308721
ACCGATC125600.019.67583322
GTATGCC55800.019.1966062
TATGCCG57200.018.8947493
CGTATGC136850.018.51190230
GAGACCG609900.018.28518530
CGAGACC612850.018.14685629
CCGAGAC624300.017.76795628
CGATCTC143400.017.51126124
GATCTCG146650.017.25210425
CTTGAAA68350.017.13300317
ATGCCGT66900.016.8508454
AGACCGA161600.016.01583520
Produced by FastQC (version 0.11.3)