##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	Crypt3_H3K4me3_ChIPSeq.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	48975219
Sequences flagged as poor quality	0
Sequence length	36
%GC	69
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.610669224368348	37.0	28.0	39.0	2.0	40.0
2	28.85842368566029	37.0	24.0	39.0	2.0	40.0
3	28.797750082546848	37.0	23.0	39.0	2.0	40.0
4	28.72386185756515	37.0	23.0	39.0	2.0	40.0
5	27.76858153508206	35.0	18.0	39.0	2.0	40.0
6	27.791147825188897	36.0	17.0	39.0	2.0	40.0
7	27.669795820616955	35.0	17.0	39.0	2.0	40.0
8	27.423233452003554	35.0	15.0	39.0	2.0	40.0
9	27.558426170590476	35.0	16.0	39.0	2.0	40.0
10	27.424355693029163	35.0	15.0	39.0	2.0	40.0
11	28.2505242498252	37.0	18.0	39.0	2.0	40.0
12	28.219077243942493	37.0	18.0	39.0	2.0	40.0
13	28.14987865597906	36.0	18.0	39.0	2.0	40.0
14	28.08139634863093	36.0	17.0	39.0	2.0	40.0
15	27.901157603807754	36.0	16.0	39.0	2.0	40.0
16	27.8944229733817	36.0	12.0	39.0	2.0	40.0
17	27.765207012142202	36.0	11.0	39.0	2.0	40.0
18	27.647038760561745	36.0	8.0	39.0	2.0	40.0
19	27.54486173915833	36.0	2.0	39.0	2.0	40.0
20	27.37535458493815	36.0	2.0	39.0	2.0	40.0
21	27.219811227388284	36.0	2.0	39.0	2.0	40.0
22	26.94688515839	35.0	2.0	39.0	2.0	40.0
23	26.89657838998127	35.0	2.0	39.0	2.0	40.0
24	26.709182290741772	35.0	2.0	39.0	2.0	40.0
25	26.452070505289623	35.0	2.0	39.0	2.0	40.0
26	25.879522886870603	34.0	2.0	39.0	2.0	40.0
27	25.61922240715248	34.0	2.0	38.0	2.0	40.0
28	25.335608198097084	33.0	2.0	38.0	2.0	40.0
29	25.094563926299134	33.0	2.0	38.0	2.0	40.0
30	24.838088401401535	33.0	2.0	38.0	2.0	39.0
31	24.696465022443288	33.0	2.0	38.0	2.0	39.0
32	24.669890562408714	33.0	2.0	38.0	2.0	39.0
33	24.628801986572025	33.0	2.0	38.0	2.0	40.0
34	24.51618950392034	33.0	2.0	38.0	2.0	39.0
35	24.36203301102135	33.0	2.0	38.0	2.0	39.0
36	24.185662446961185	33.0	2.0	38.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.40868952861710284
1	2	-0.33866885574865435
1	3	-0.1130154425162857
1	4	-0.06676864615597822
1	5	0.9356403825046264
1	6	0.8924630546149999
1	7	0.8678812418181465
1	8	0.977482993400745
1	9	0.8338223702069385
1	10	0.9096392446797665
1	11	0.19331696910366958
1	12	0.1143320687705014
1	13	0.18448430621505096
1	14	0.16632866360001586
1	15	0.27581894181149025
1	16	-0.21443418200497888
1	17	0.31678632966237075
1	18	0.3416500839798253
1	19	0.36678435062288983
1	20	0.4550924460431247
1	21	0.47677684957429634
1	22	0.5527503818191484
1	23	0.48504914957861445
1	24	0.6181359351615896
1	25	0.7637186540726191
1	26	1.0388528490038205
1	27	1.0536240706797138
1	28	1.2925409798993606
1	29	1.3095860332001088
1	30	1.2606267569155776
1	31	1.4251325704664275
1	32	1.4881866986110666
1	33	1.4368495280452365
1	34	1.4561156100578323
1	35	1.4599649339749519
1	36	1.520590279842832
2	1	-0.6026945569593067
2	2	-0.24132770490899347
2	3	-0.2889863293524968
2	4	-0.18591031579963513
2	5	0.8543809484348763
2	6	0.7700986622703176
2	7	0.7630641788976256
2	8	0.7602000649818628
2	9	0.7532398128841535
2	10	0.844497748243878
2	11	0.19543577973977122
2	12	0.13615735709213794
2	13	0.1347635942815799
2	14	0.12239613001991856
2	15	0.29880913361647643
2	16	0.2605218978127617
2	17	0.33917626127248823
2	18	0.38688769777746757
2	19	0.3543576035331739
2	20	0.476535115588856
2	21	0.5203483456223381
2	22	0.6846326838480934
2	23	0.6149630108502535
2	24	0.6173868898932859
2	25	0.6820429574213946
2	26	0.9109731084848463
2	27	0.7587560755363931
2	28	0.9716458128601033
2	29	1.0866587300178985
2	30	1.2375126858988637
2	31	1.1784545125070558
2	32	1.103470705753093
2	33	1.3349086872669105
2	34	1.2962341281379324
2	35	1.1666669687486007
2	36	1.3419138150540242
3	1	-0.5224493232077307
3	2	-0.34929574706132627
3	3	-0.30052660086530025
3	4	-0.12431808857813209
3	5	0.8165727386210158
3	6	0.8131427165215577
3	7	0.6748995460060279
3	8	0.8686631171910584
3	9	0.7953587077125057
3	10	0.8657043575592986
3	11	0.14883300751906958
3	12	0.022374124507877013
3	13	0.18159032682930842
3	14	0.1845355084377971
3	15	0.31809054010818016
3	16	0.3286280287868557
3	17	0.34342466158092577
3	18	0.3635075887702115
3	19	0.3640483279933129
3	20	0.48741735830335386
3	21	0.44277349597414783
3	22	0.6978328818681518
3	23	0.6284717757637779
3	24	0.5459099876062012
3	25	0.7630512640201168
3	26	0.9973335496979168
3	27	0.814897909860079
3	28	1.0570602562363973
3	29	0.9890039286178052
3	30	1.2595056521573724
3	31	1.2517982319704863
3	32	1.0727133972983616
3	33	1.3118373857120105
3	34	1.2238215820696503
3	35	1.272549275110972
3	36	1.4169647709398312
4	1	-0.508005923506289
4	2	-0.23319985954109868
4	3	-0.17911289242034556
4	4	-0.036643861393979904
4	5	0.9011652714781455
4	6	0.8915118331198002
4	7	0.7874079999940697
4	8	0.9133920102188462
4	9	0.7664899881045244
4	10	0.8916563785540284
4	11	0.13796371650686723
4	12	0.04175797364505485
4	13	0.17516534814106421
4	14	0.03194875222648008
4	15	0.3070059397770528
4	16	0.30150157942176037
4	17	0.3513997151728496
4	18	0.21671207414948412
4	19	0.23693217294199798
4	20	0.3879649840364223
4	21	0.4581455453061132
4	22	0.6507271059662507
4	23	0.6271083445810675
4	24	0.4926417214979679
4	25	0.685406410321189
4	26	0.8905798878200528
4	27	0.8902745347741856
4	28	1.0788635201019225
4	29	1.360597274916497
4	30	1.104987737994449
4	31	1.0281855530165913
4	32	0.9638086512157784
4	33	1.2416511961174592
4	34	1.1929387515675565
4	35	0.9853660181648785
4	36	1.125448070970915
5	1	-0.4083990683667018
5	2	-0.0837344924889365
5	3	-0.11479203974665353
5	4	0.02351474018066213
5	5	0.9989003534393461
5	6	0.8923938199757337
5	7	0.8269505716541232
5	8	0.7926769488796381
5	9	0.8870393296534864
5	10	0.9512844875198745
5	11	0.22803474505000665
5	12	0.13433083955619196
5	13	0.22380891100768352
5	14	0.247892326158901
5	15	0.3544921455785932
5	16	0.38738322029445627
5	17	0.4130930781739579
5	18	0.42756762786030933
5	19	0.4417272031754216
5	20	0.5585054847930166
5	21	0.6010893697576947
5	22	0.7166739446567618
5	23	0.6902137621326183
5	24	0.6664605711643468
5	25	0.664913592673102
5	26	0.8430524363614218
5	27	0.6887745860600525
5	28	0.8001048062366038
5	29	0.9275973634531809
5	30	0.9909572768936883
5	31	1.2631812179895512
5	32	1.1906401644094586
5	33	1.1583165140031753
5	34	1.279346539334771
5	35	1.1515474874417357
5	36	1.278299660437483
6	1	-0.3702813980986228
6	2	0.0459649496484964
6	3	0.10343850346317751
6	4	0.15629688791026908
6	5	1.1291530797104947
6	6	1.0089225939266484
6	7	0.9893695313112154
6	8	0.926083223126895
6	9	1.0341049880596955
6	10	1.0510643600764915
6	11	0.39058511049822187
6	12	0.326218165421178
6	13	0.322441678897583
6	14	0.32452480426906405
6	15	0.4485417006581862
6	16	0.5542066745404775
6	17	0.5947417111772104
6	18	0.5994674656519052
6	19	0.5348274340506265
6	20	0.6846242138183598
6	21	0.7525067746054326
6	22	0.8921791521547249
6	23	0.7963570497215606
6	24	0.850425421842413
6	25	0.9928819962058242
6	26	1.1910952962044234
6	27	1.2147985561935073
6	28	1.3309077073724076
6	29	1.2186536410094568
6	30	1.3278034011558155
6	31	1.3514998145724633
6	32	1.164273141925733
6	33	1.3565350290943599
6	34	1.378848015114169
6	35	1.1372989770856137
6	36	1.4484641091081656
7	1	-0.3101083884905371
7	2	0.0828826144733803
7	3	0.18386976053595916
7	4	0.18825382138100366
7	5	1.217365976374655
7	6	1.1018000628938331
7	7	1.0482314789618101
7	8	1.1216999077756071
7	9	1.113718512602567
7	10	1.1707598246467477
7	11	0.4560865621079593
7	12	0.4300782664488061
7	13	0.49513455012596097
7	14	0.4800256560289533
7	15	0.589893089740972
7	16	0.6202329275205614
7	17	0.6555071733830395
7	18	0.7002013536984038
7	19	0.6987669358309461
7	20	0.8042996385618153
7	21	0.7928856806568412
7	22	0.9092943747030553
7	23	0.8895045856163861
7	24	0.8948285672075507
7	25	0.9705270986738981
7	26	1.0778877591852165
7	27	1.1574640737307789
7	28	0.9584148040265106
7	29	1.1986662674452973
7	30	0.9280263408666016
7	31	1.5028643922822056
7	32	1.2440674283962672
7	33	1.4249093041973353
7	34	1.4623147007177977
7	35	1.324344130177785
7	36	1.5976451526693687
8	1	-0.30022535386938287
8	2	-0.026695061114157426
8	3	0.07215660798969381
8	4	0.13493594178359558
8	5	1.1567508209327677
8	6	1.0162796942852914
8	7	0.9274578829554692
8	8	0.9685284505112186
8	9	0.9876454856537897
8	10	1.0815483722039438
8	11	0.3653200422587233
8	12	0.32275766602139555
8	13	0.34002009508218833
8	14	0.30017700720176066
8	15	0.4217785404315535
8	16	0.49370189842456114
8	17	0.5574349320372534
8	18	0.5389861022483409
8	19	0.5228008363198136
8	20	0.6489261341794794
8	21	0.7485637417769659
8	22	0.7922068061045557
8	23	0.7978082748571431
8	24	0.7915784262353363
8	25	0.5705894316979965
8	26	1.077985876226073
8	27	1.0735032132804854
8	28	1.1049824998510793
8	29	1.2429946964017233
8	30	1.2504132964386123
8	31	1.508367301762469
8	32	1.3951275800978529
8	33	1.3993126851607727
8	34	1.3815396942848217
8	35	1.3134938970690726
8	36	1.34004688732659
9	1	-0.3731837961130253
9	2	-0.24400730492634892
9	3	-0.098748429795112
9	4	-0.3121067396946806
9	5	0.9317475750672131
9	6	0.89636661502675
9	7	0.9284048116332428
9	8	0.7338503980766653
9	9	0.828109722242985
9	10	0.9126756050008353
9	11	0.235869812091984
9	12	0.28209889763214946
9	13	0.3358333328283436
9	14	0.2800042603458692
9	15	0.40100513374174085
9	16	0.4566123865077145
9	17	0.4808910411855116
9	18	0.4645158670042626
9	19	0.49256492855163714
9	20	0.5572138814302114
9	21	0.6729581290851563
9	22	0.7278131549522513
9	23	0.758851286974263
9	24	0.7904626027567367
9	25	0.8052013689899233
9	26	0.9391297252305648
9	27	0.884830664009165
9	28	0.9329295551465684
9	29	0.8000063108390627
9	30	1.1305237368390664
9	31	1.1502885895708452
9	32	1.17290091330932
9	33	1.1006145348471286
9	34	1.131970435781266
9	35	1.071389071490394
9	36	1.1956653231841798
10	1	-0.26160959824764163
10	2	0.008155987306800228
10	3	0.028780152222104505
10	4	0.06523568811221025
10	5	1.0177234085427074
10	6	1.0290265394383802
10	7	1.0474746604565084
10	8	1.141475535110338
10	9	1.036824176138179
10	10	1.1086813759532674
10	11	0.41748333481940847
10	12	0.30903869439253384
10	13	0.4188753679486368
10	14	0.43557706109593397
10	15	0.5334273138234913
10	16	0.5259959217714858
10	17	0.581962803190585
10	18	0.5889409305786302
10	19	0.5207035153823867
10	20	0.6728769409210074
10	21	0.6828953812178575
10	22	0.7584389955025799
10	23	0.7053251742079496
10	24	0.773432932404539
10	25	0.8038927932937803
10	26	0.9732582390119511
10	27	0.7721082727401765
10	28	0.7721113484131763
10	29	0.9094227226131792
10	30	1.090853521843318
10	31	1.1554374058247276
10	32	1.184486387938417
10	33	0.8981325360134846
10	34	1.058795831740806
10	35	0.9277264407877119
10	36	1.0383924618682059
11	1	-0.41895334858070044
11	2	-0.004677723847937187
11	3	0.04935973505185132
11	4	0.02157827833638848
11	5	1.14664607665458
11	6	1.0223494120747318
11	7	1.0341617182882281
11	8	1.029740143849363
11	9	1.0551673386898415
11	10	1.0928253466910256
11	11	0.4457473866848538
11	12	0.4020888988104332
11	13	0.37771979931942923
11	14	0.41947209069283176
11	15	0.5323209205950725
11	16	0.5959829005218289
11	17	0.5950981719634605
11	18	0.6196461670021058
11	19	0.646743963055453
11	20	0.6911670006476953
11	21	0.7581974784730292
11	22	0.9036826956370838
11	23	0.8084217110404737
11	24	0.8903392811896502
11	25	0.9356250617995663
11	26	0.9827465521273098
11	27	0.9557099711779458
11	28	0.9969921801318407
11	29	1.2249790526404425
11	30	1.3960963000722764
11	31	1.4606100952523349
11	32	1.319351763084697
11	33	1.3654522635131272
11	34	1.232966849481894
11	35	1.170143741064603
11	36	1.2473532238631044
12	1	-0.15143815745354416
12	2	0.12809970283787564
12	3	0.14824977135214112
12	4	0.20766876893390318
12	5	1.239408404748815
12	6	1.132483823741115
12	7	1.1415603128011647
12	8	1.1360095598563227
12	9	1.1257130130679265
12	10	1.1561351954099806
12	11	0.43035220350883563
12	12	0.3309531510597843
12	13	0.44995439087319156
12	14	0.4893728746935402
12	15	0.5470864410216372
12	16	0.558771750495918
12	17	0.5798729361249855
12	18	0.4916537371937473
12	19	0.6463782733903578
12	20	0.6338506098754664
12	21	0.7969451946078969
12	22	0.8835164394533273
12	23	0.799792229254443
12	24	0.9436623554639958
12	25	0.9412516948739231
12	26	1.1937311670661224
12	27	1.1048990592486447
12	28	1.1188528029326719
12	29	1.232649506412148
12	30	1.4208504568173232
12	31	1.424373412952626
12	32	1.122320944026736
12	33	1.300560749590442
12	34	1.2347654531300698
12	35	1.2086458461206355
12	36	1.2991605765325112
13	1	-0.3654959657892469
13	2	-0.12559500487408926
13	3	-0.14689586328858795
13	4	-0.11304695991729119
13	5	0.9552827294223505
13	6	0.8139847285720236
13	7	0.7647302414918293
13	8	0.7431579178758909
13	9	0.7989040609118128
13	10	0.8841487548503153
13	11	0.23679988856219225
13	12	0.1528894727944632
13	13	0.22110891263575638
13	14	0.128949518347099
13	15	0.3624525510249832
13	16	0.32563661150553713
13	17	0.4130240752820349
13	18	0.4009000457715466
13	19	0.40260954640134017
13	20	0.4642641042223552
13	21	0.5234496301522604
13	22	0.5101754613991965
13	23	0.590479630237823
13	24	0.5104796021219329
13	25	0.6203303975093277
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117	17	0.8663002367548671
117	18	0.9124895368160715
117	19	0.8841408366834891
117	20	0.9590164091544864
117	21	1.073382851624853
117	22	1.1049836659922505
117	23	1.153264048346184
117	24	1.1915299512490662
117	25	1.338158033711725
117	26	1.5179044472788092
117	27	1.54087233366349
117	28	1.669816945577324
117	29	1.6504574837715964
117	30	1.7169471163953283
117	31	1.9592818901760758
117	32	1.9535110012934247
117	33	1.7702938688167187
117	34	1.6981748753072416
117	35	1.782078860309955
117	36	1.875243677732989
118	1	0.09912702869194234
118	2	0.3809571548593027
118	3	0.4434310141474107
118	4	0.5423562122993886
118	5	1.449979559643868
118	6	1.422097776735935
118	7	1.415205443168734
118	8	1.5491974997563709
118	9	1.4479813946229
118	10	1.4861602360584278
118	11	0.8197255257961089
118	12	0.7697708001302068
118	13	0.7787606322521192
118	14	0.8153423976129552
118	15	0.8208883966515756
118	16	0.8620666620877628
118	17	0.8055796888710596
118	18	0.9355635486395997
118	19	0.9511243427911182
118	20	1.0341402349223614
118	21	1.135557331488254
118	22	1.229610077718693
118	23	1.2066316383309506
118	24	1.2587893846332676
118	25	1.4067034551449389
118	26	1.578243923192563
118	27	1.576859181204597
118	28	1.8160382956935202
118	29	1.9906931155100374
118	30	2.0327420158747778
118	31	2.140725518459014
118	32	2.036048207407866
118	33	1.8884429399793845
118	34	1.8048564540688794
118	35	1.9156597221550697
118	36	2.004494793161278
119	1	0.14883807504066482
119	2	0.3830393779800403
119	3	0.3883021862441929
119	4	0.47113733895458054
119	5	1.403281171534477
119	6	1.303090107967762
119	7	1.2493787963354954
119	8	1.2924037069403589
119	9	1.2689020300174931
119	10	1.3085963289269635
119	11	0.6023860101081588
119	12	0.5875750228295438
119	13	0.5949999440706932
119	14	0.5748246018202785
119	15	0.6711744833441458
119	16	0.6749032536486865
119	17	0.6444904590945981
119	18	0.7233144767231217
119	19	0.6584469846455114
119	20	0.7681588987521053
119	21	0.8612175774162125
119	22	0.9383855087281461
119	23	0.9483650538166764
119	24	1.0148264854957851
119	25	1.1396260765289554
119	26	1.3410931512413313
119	27	1.3072367764103205
119	28	1.506325608073844
119	29	1.6860801972767874
119	30	1.6444576098058903
119	31	1.7679964742263827
119	32	1.7323570292911228
119	33	1.6670744743891674
119	34	1.553194992629951
119	35	1.588726284685496
119	36	1.7267626369628566
120	1	-0.05920055813208336
120	2	-0.07404008808563134
120	3	-0.03243885868640817
120	4	0.10967237746004344
120	5	1.0621889086506862
120	6	0.9590563724301084
120	7	0.9606644412355969
120	8	0.9078071760993645
120	9	0.9374516810104474
120	10	0.9844833387087633
120	11	0.35793459040422704
120	12	0.30128094013523565
120	13	0.36567219345584334
120	14	0.41787275092523757
120	15	0.4579565406884498
120	16	0.5063458746367289
120	17	0.4305883837900346
120	18	0.5459103426614753
120	19	0.5372883877063082
120	20	0.6045040550449698
120	21	0.6697928497832528
120	22	0.7568961536744894
120	23	0.7952631054298713
120	24	0.8098516074481665
120	25	0.9492532369703746
120	26	1.1221433793176985
120	27	1.1675125035212233
120	28	1.3330741656155958
120	29	1.4521781690362268
120	30	1.598250015486503
120	31	1.7008381532494852
120	32	1.5705263276992554
120	33	1.5457034637523535
120	34	1.4624835056017886
120	35	1.5036605901450173
120	36	1.5943816318060406
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	9743864.0
3	232752.0
4	381975.0
5	581633.0
6	188909.0
7	140354.0
8	156808.0
9	176990.0
10	178347.0
11	165512.0
12	181234.0
13	197097.0
14	206335.0
15	206377.0
16	210769.0
17	220391.0
18	227020.0
19	235599.0
20	252566.0
21	286240.0
22	326682.0
23	366628.0
24	405846.0
25	449205.0
26	502538.0
27	561991.0
28	633062.0
29	727313.0
30	849308.0
31	1014224.0
32	1246588.0
33	1567277.0
34	2005775.0
35	2610509.0
36	3435683.0
37	4577247.0
38	5479095.0
39	8031534.0
40	13942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.61520400368549	19.62475587628794	11.234914716636357	34.52512540339021
2	36.65017230173922	19.80849068821161	14.928235383136625	28.61310162691254
3	34.582604625374366	18.412406446663006	15.143018233989707	31.861970693972925
4	36.54064718095993	17.65795522906068	14.636281804860216	31.165115785119173
5	35.7593791065802	18.676871915677907	15.626554609526647	29.93719436821525
6	35.37855661368125	16.77845801026618	13.524609202251714	34.318376173800864
7	34.61094533652801	17.349710853501836	15.318926482858963	32.72041732711119
8	35.65131004719734	16.02110207200648	15.200532914970283	33.12705496582589
9	34.812144780365614	15.672686378269846	14.770404326296335	34.744764515068205
10	35.14916918526298	17.293190621223253	14.199757257585762	33.35788293592801
11	36.63827111397303	16.082554562957814	14.29882878228863	32.98034554078051
12	36.175341238124716	15.841582219037969	14.706278170358	33.27679837247931
13	35.10709941521842	16.788033026126637	14.815947938035318	33.28891962061963
14	34.82883994529099	16.827054335284277	14.648086972601565	33.69601874682317
15	34.671588638998024	15.992743267795209	15.647251421454378	33.688416671752385
16	35.779854983110624	15.875898332248845	14.554900600473578	33.78934608416696
17	34.7445160294476	15.996048431396714	14.471218926868001	34.788216612287684
18	34.84764272758841	16.017187439427843	14.515492862486463	34.61967697049729
19	36.05448205882129	15.76484424764429	14.39312799692987	33.787545696604546
20	35.204097508913485	16.63780000597604	14.294691600813211	33.86341088429726
21	35.77465297540277	16.006658200713364	14.219003436177408	33.99968538770645
22	35.107161241447486	16.734340960546987	14.238875831833228	33.9196219661723
23	34.8898865067442	15.867819077458728	15.14241734663902	34.099877069158055
24	35.25521317858345	15.817298219521955	14.2418204163327	34.685668185561894
25	36.28442375162026	15.881393282809029	14.141593435504129	33.692589530066584
26	36.57245240012939	15.837903790164454	14.270629774016147	33.319014035689996
27	36.20817337401846	16.461640372853655	14.184248034091373	33.14593821903651
28	36.2367668111721	16.484348933968775	14.031904291820865	33.24697996303826
29	37.169518461489034	15.596430045551275	14.162656523290137	33.07139496966956
30	36.49643197325683	16.338320845328987	14.05679487197444	33.10845230943974
31	37.15944483455545	15.638512021886422	14.05253365768159	33.149509485876536
32	36.0072832019312	15.595160254644302	14.190874061471584	34.20668248195291
33	36.52652545507844	15.792051008667784	14.074849294079709	33.60657424217406
34	36.51154516181903	15.498950278816936	14.21919003761482	33.77031452174922
35	35.63355785012673	15.631594397367662	14.814959508266087	33.91988824423952
36	35.74640741497062	15.684607069211264	14.742574687241989	33.82641082857613
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	104669.0
1	93344.5
2	82020.0
3	82020.0
4	57588.5
5	33157.0
6	33157.0
7	30598.0
8	28039.0
9	28039.0
10	29847.0
11	31655.0
12	31655.0
13	35841.5
14	40028.0
15	47180.5
16	54333.0
17	54333.0
18	63953.5
19	73574.0
20	73574.0
21	89705.0
22	105836.0
23	105836.0
24	130748.5
25	155661.0
26	191981.0
27	228301.0
28	228301.0
29	279565.0
30	330829.0
31	330829.0
32	380970.0
33	431111.0
34	431111.0
35	481087.5
36	531064.0
37	531064.0
38	566543.0
39	602022.0
40	623393.5
41	644765.0
42	644765.0
43	664768.0
44	684771.0
45	684771.0
46	714461.5
47	744152.0
48	744152.0
49	802349.0
50	860546.0
51	954255.0
52	1047964.0
53	1047964.0
54	1193827.0
55	1339690.0
56	1339690.0
57	1760156.5
58	2180623.0
59	2180623.0
60	2244443.0
61	2308263.0
62	2308263.0
63	2606632.0
64	2905001.0
65	3273911.5
66	3642822.0
67	3642822.0
68	3994546.5
69	4346271.0
70	4346271.0
71	4611549.0
72	4876827.0
73	4876827.0
74	4968928.5
75	5061030.0
76	4919764.0
77	4778498.0
78	4778498.0
79	4410897.5
80	4043297.0
81	4043297.0
82	3526943.0
83	3010589.0
84	3010589.0
85	2467616.0
86	1924643.0
87	1924643.0
88	1488988.5
89	1053334.0
90	761596.0
91	469858.0
92	469858.0
93	319108.5
94	168359.0
95	168359.0
96	106531.5
97	44704.0
98	44704.0
99	25808.5
100	6913.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.818728345043235
2	0.9381438396426568
3	0.8262627677070724
4	0.8390039052199032
5	1.0217943078518956
6	0.9261275585107644
7	0.896567711111205
8	1.0897756271391048
9	0.8867750034971768
10	0.9333740804711869
11	1.0964728917291824
12	0.9707685023317609
13	0.9319407025009934
14	0.9429789379808592
15	1.066980425345316
16	1.0035422199949735
17	0.9739823725954141
18	0.9787378388241612
19	0.9791645852568827
20	0.9832727853651864
21	0.9748440328566983
22	1.0373041925550144
23	1.0358646890379397
24	0.9614780895619885
25	0.9450840842590208
26	1.030110758667562
27	0.904183807733458
28	0.8711364823095533
29	0.864394297042347
30	0.869204893193025
31	0.8676530879831288
32	0.8680798344158502
33	0.8621013006598297
34	0.8584361817759303
35	0.8532907223957488
36	0.8506159002576384
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	4.8975219E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.87285176350794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.06900025495823	49.71293532118761
2	8.714634707879593	10.958278723232702
3	4.469527533879267	8.430358262715252
4	2.6777404241156004	6.734287069862921
5	1.752744258534409	5.510001497308678
6	1.1369796760484001	4.289109277818741
7	0.7210965256102339	3.173617647331099
8	0.4794729452461329	2.411666512885814
9	0.3228283631615169	1.8267425839898968
>10	0.6495953815905583	5.188823110787284
>50	0.004089846445598869	0.18120030072756738
>100	0.0021516410846185914	0.22031415054153458
>500	6.599398751242863E-5	0.027430447683759806
>1k	3.034824770057704E-5	0.04132233669300799
>5k	3.2384230163687027E-6	0.01160977892237897
>10k+	3.886078757511367E-5	1.2823029783117799
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT	323239	0.6600052161073542	Illumina Paired End PCR Primer 2 (96% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	63472	0.1296002372138448	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.023960423740014E-4	0.0	4.0836979207790785E-6	2.041848960389539E-5
2	0.0016314373193512418	0.0	4.0836979207790785E-6	4.69625260889594E-5
3	0.005437443781517342	0.0	6.125546881168617E-6	5.921361985129663E-5
4	0.0070096674810172874	0.0	8.167395841558157E-6	8.167395841558156E-5
5	0.009804958707790567	0.0	8.167395841558157E-6	9.188320321752926E-5
6	0.011703878240952838	0.0	1.2251093762337235E-5	1.0005059905908742E-4
7	0.013817191914956011	0.0	1.2251093762337235E-5	1.0617614594025604E-4
8	0.015614019000098804	2.0418489603895393E-6	1.2251093762337235E-5	1.204690886629828E-4
9	0.01776408595538899	2.0418489603895393E-6	1.4292942722726773E-5	1.3680388034609912E-4
10	0.02015509108800514	2.0418489603895393E-6	1.4292942722726773E-5	1.4497127618765728E-4
11	0.02440009507665499	2.0418489603895393E-6	1.4292942722726773E-5	1.5926421891038404E-4
12	0.027961079663574348	2.0418489603895393E-6	1.4292942722726773E-5	1.7559901059350036E-4
13	0.03130971195861319	4.0836979207790785E-6	1.4292942722726773E-5	1.878501043558376E-4
14	0.03393348787271375	4.0836979207790785E-6	1.837664064350585E-5	1.939756512370062E-4
15	0.0845366306580477	4.0836979207790785E-6	1.837664064350585E-5	2.0214304707856436E-4
16	0.088495775792243	4.0836979207790785E-6	1.837664064350585E-5	2.08268593959733E-4
17	0.0949970228821233	4.0836979207790785E-6	1.837664064350585E-5	2.2256153668245976E-4
18	0.0992542779645355	4.0836979207790785E-6	1.837664064350585E-5	2.4298002628635515E-4
19	0.10626598729451317	4.0836979207790785E-6	1.837664064350585E-5	2.572729690090819E-4
20	0.8424423788691991	4.0836979207790785E-6	1.837664064350585E-5	2.715659117318087E-4
21	0.8618766972741868	4.0836979207790785E-6	1.837664064350585E-5	2.940262502960936E-4
22	0.8671936719670411	4.0836979207790785E-6	1.837664064350585E-5	3.246539847019367E-4
23	0.8727127897069741	4.0836979207790785E-6	1.837664064350585E-5	3.4711432326622166E-4
24	0.8781134802072044	4.0836979207790785E-6	2.041848960389539E-5	3.593654170285589E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAT	5735	0.0	24.375755	28
TCGTATG	5875	0.0	23.97462	29
TCTCGTA	6180	0.0	22.642656	27
ATCTCGT	6250	0.0	22.23518	26
ATGCCGA	59470	0.0	21.251152	14
AATGCCG	60700	0.0	21.010555	13
CCGAGAT	53330	0.0	20.938444	17
CGAGATC	53105	0.0	20.931772	18
GAATGCC	61725	0.0	20.703362	12
CAGGAAT	62555	0.0	20.484438	9
AGATCGG	54855	0.0	20.437267	20
CGGTTCA	57980	0.0	20.346653	1
GTATGCC	5590	0.0	20.179636	2
GAGATCG	55440	0.0	20.151014	19
TATGCCG	5670	0.0	20.064203	3
TTCAGCA	60230	0.0	19.873251	4
GTTCAGC	60715	0.0	19.72122	3
AGGAATG	65390	0.0	19.698126	10
GGAATGC	65370	0.0	19.688833	11
ATCGGAA	56925	0.0	19.67164	22
>>END_MODULE
