FastQCFastQC Report
Sun 14 Aug 2016
Crypt3_H3K4me3_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCrypt3_H3K4me3_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48975219
Sequences flagged as poor quality0
Sequence length36
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT3232390.6600052161073542Illumina Paired End PCR Primer 2 (96% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN634720.1296002372138448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT57350.024.37575528
TCGTATG58750.023.9746229
TCTCGTA61800.022.64265627
ATCTCGT62500.022.2351826
ATGCCGA594700.021.25115214
AATGCCG607000.021.01055513
CCGAGAT533300.020.93844417
CGAGATC531050.020.93177218
GAATGCC617250.020.70336212
CAGGAAT625550.020.4844389
AGATCGG548550.020.43726720
CGGTTCA579800.020.3466531
GTATGCC55900.020.1796362
GAGATCG554400.020.15101419
TATGCCG56700.020.0642033
TTCAGCA602300.019.8732514
GTTCAGC607150.019.721223
AGGAATG653900.019.69812610
GGAATGC653700.019.68883311
ATCGGAA569250.019.6716422
Produced by FastQC (version 0.11.3)