FastQCFastQC Report
Sun 14 Aug 2016
Crypt3_H3K27ac_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCrypt3_H3K27ac_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102591635
Sequences flagged as poor quality0
Sequence length36
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT4816580.46949051937811503Illumina Paired End PCR Primer 2 (96% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1131120.11025460311652115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT87350.022.99366828
TCGTATG89150.022.76148829
TATGCCG131850.021.8161133
TCTCGTA92150.021.59535827
ATGCCGA894350.021.47515714
AATGCCG917400.021.26684413
CGAGATC817250.020.51570118
ATCTCGT97400.020.36043526
CCGAGAT832650.020.33000817
CGGTTCA886200.020.2400171
GAATGCC978800.020.0157412
AGATCGG876100.019.42294920
GAGATCG873400.019.3363119
GTTCAGC964600.019.2291953
GTATGCC151650.019.2048422
ATCGGAA884600.019.18578322
AGCGGTT868200.019.18286130
TCGGAAG898450.019.14587623
GGAATGC1032300.019.12001611
ATGCCGT153450.018.871934
Produced by FastQC (version 0.11.3)