FastQCFastQC Report
Sun 14 Aug 2016
Crypt2_H3K27ac_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCrypt2_H3K27ac_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38540916
Sequences flagged as poor quality0
Sequence length36
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT1326780.34425232654044857Illumina Paired End PCR Primer 2 (96% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN649050.1684054421539955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA206150.024.35500114
AATGCCG211100.023.97600713
CGGTTCA206050.023.9711991
AGCGGTT182500.022.94965430
ATCGGAA196000.021.80328222
TCGTATG31400.021.52031529
CTCGTAT31350.021.42429728
TCTCGTA31650.021.27092427
TCGGAAG204650.020.98482923
GAGCGGT202900.020.81869329
GAATGCC246550.020.75931212
TGCCGAG243900.020.67944115
GATCGGA209400.020.50724821
GTTCAGC243050.020.428763
AAGAGCG210650.020.33844827
CGGAAGA215500.019.9948324
ACCGATC34550.019.73801622
GACCGAT34700.019.60138321
GGTTCAG260300.019.1732252
AGATCGG223500.019.0957520
Produced by FastQC (version 0.11.3)