FastQCFastQC Report
Sun 14 Aug 2016
Crypt1_H3K4me3_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCrypt1_H3K4me3_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44684593
Sequences flagged as poor quality0
Sequence length36
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT6391511.4303610195129224Illumina Paired End PCR Primer 2 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCG3874560.867090811367578Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG1900310.4252718604822025Illumina Paired End PCR Primer 2 (100% over 36bp)
CGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC800390.17911990381114135Illumina Paired End PCR Primer 2 (100% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN654860.14655163134192584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT98250.026.06980928
TCGTATG100500.025.64246429
TCTCGTA101450.025.23511527
GCGGTTC812550.023.89873311
ATCTCGT107350.023.7495126
CGTATGC109250.023.7248330
GACCGAT113250.023.38704921
ACCGATC114400.022.9362422
AGATCGG949800.022.3289620
CCGATCT125000.020.99308223
AGACCGA153350.017.74878720
CCGAGAT1317200.016.18729417
CGAGATC1322050.016.04454618
GAGATCG1354700.015.69405619
GATCTCG172400.015.52003125
AATGCCG1587600.015.36561713
ATGCCGA1571750.015.34813114
CGGTTCA1544450.015.3160041
GAATGCC1606200.015.21721312
GAGACCG432250.015.12811530
Produced by FastQC (version 0.11.3)