FastQCFastQC Report
Sun 14 Aug 2016
Crypt1_H3K27ac_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCrypt1_H3K27ac_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60045762
Sequences flagged as poor quality0
Sequence length36
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1105250.18406794471190158No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT720350.11996683462856214Illumina Paired End PCR Primer 2 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG182250.016.13060420
ATGCCGA212050.016.03532814
CGGTTCA204050.015.351251
AATGCCG226250.015.31394413
TCGTATG23200.014.60815429
ATCGGAA203700.014.42251122
CTCGTAT23300.014.30588528
AGCGGTT202300.014.03846530
TCTCGTA24000.013.88993727
GCGGTTC119750.013.74956811
GATCGGA215150.013.66056421
TGCCGAG255400.013.48753515
TCGGAAG224400.013.35122223
GAGCGGT222300.012.93175629
GACCGAT29050.012.70377221
CGGAAGA239450.012.69943624
AAGAGCG237050.012.4728127
GAATGCC284950.012.44102412
GTTCAGC260850.012.2468143
CGAGATC245600.012.11247518
Produced by FastQC (version 0.11.3)