FastQCFastQC Report
Sun 14 Aug 2016
C91_input.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameC91_input.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36388650
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCG1150080.3160545939461893Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG384180.10557687630621086Illumina Paired End PCR Primer 2 (100% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT369830.1016333389669581Illumina Paired End PCR Primer 2 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC213850.023.62136711
TCGGAAG248250.020.6376673
AGCGGTT253800.019.91424210
GAGCGGT255200.019.8343779
ATCGGAA264100.019.3538252
AGAGCGG264750.019.3511498
AAGAGCG265800.019.3029177
CGGTTCA265200.019.05327612
ATGCCGA262100.018.98686825
AATGCCG264950.018.89587424
CGGAAGA272900.018.768084
GATCGGA272050.018.742871
TGCCGAG269800.018.45067626
CTCGTAT15550.017.45869628
GAGACCG78250.016.6005230
TCTCGTA16650.016.57488627
GTTCAGC309950.016.38475214
GGTTCAG314750.016.22535313
CCGAGAC81350.016.02233928
GAATGCC315850.015.9595823
Produced by FastQC (version 0.11.3)