##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	C91_H3K4me1_ChIPSeq.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22562708
Sequences flagged as poor quality	0
Sequence length	152
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.319139262893444	34.0	34.0	34.0	34.0	34.0
2	33.248962934768294	34.0	34.0	34.0	34.0	34.0
3	33.24385805108146	34.0	34.0	34.0	34.0	34.0
4	33.25842775610091	34.0	34.0	34.0	34.0	34.0
5	33.25587748598262	34.0	34.0	34.0	34.0	34.0
6	33.32849926524777	34.0	34.0	34.0	34.0	34.0
7	33.30279206733518	34.0	34.0	34.0	34.0	34.0
8	33.282234871807056	34.0	34.0	34.0	33.0	34.0
9	33.277691933078245	34.0	34.0	34.0	33.0	34.0
10-14	33.306283465619465	34.0	34.0	34.0	33.4	34.0
15-19	33.27330394028944	34.0	34.0	34.0	33.0	34.0
20-24	33.07167558078578	34.0	34.0	34.0	33.0	34.0
25-29	32.79865481572513	34.0	34.0	34.0	32.0	34.0
30-34	32.57970151455224	34.0	34.0	34.0	31.8	34.0
35-39	32.26864628128858	34.0	34.0	34.0	31.0	34.0
40-44	31.83288799376387	34.0	33.8	34.0	30.0	34.0
45-49	31.11299390126398	34.0	33.0	34.0	26.6	34.0
50-54	30.5007586146131	34.0	32.2	34.0	23.0	34.0
55-59	29.773718970258358	34.0	31.6	34.0	13.4	34.0
60-64	28.768309477745312	33.6	30.6	34.0	2.0	34.0
65-69	27.606225644545862	33.0	28.8	34.0	2.0	34.0
70-74	26.301412064544735	33.0	26.2	34.0	2.0	34.0
75-79	29.78830701527494	33.2	28.4	34.0	20.8	34.0
80-84	33.024379405167146	34.0	34.0	34.0	33.0	34.0
85-89	32.95313907355447	34.0	34.0	34.0	33.0	34.0
90-94	32.850641465554574	34.0	34.0	34.0	33.0	34.0
95-99	32.64945745874121	34.0	34.0	34.0	32.2	34.0
100-104	32.448743200505895	34.0	34.0	34.0	32.0	34.0
105-109	32.3501060510999	34.0	34.0	34.0	31.8	34.0
110-114	32.09585575454861	34.0	34.0	34.0	31.0	34.0
115-119	31.798910325835	34.0	34.0	34.0	30.2	34.0
120-124	31.17882616749727	34.0	33.0	34.0	27.0	34.0
125-129	30.576810904081196	34.0	32.2	34.0	23.6	34.0
130-134	30.28257283655845	34.0	32.0	34.0	20.6	34.0
135-139	29.5420005878727	34.0	31.6	34.0	7.6	34.0
140-144	28.319434652968074	33.2	29.8	34.0	2.0	34.4
145-149	27.240459824237412	33.0	28.8	34.0	2.0	34.0
150-152	25.834214758263943	32.666666666666664	24.333333333333332	34.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1	1	-0.06555295042137033
1	2	-0.0677686348257609
1	3	-0.11919672883045962
1	4	-0.06032841024016733
1	5	-0.04694407161828451
1	6	-0.07138512305795786
1	7	-0.06832367089104707
1	8	-0.05974381516343641
1	9	-0.0430674210120614
1	10-14	-0.02905731989493887
1	15-19	-0.023763105493259218
1	20-24	0.06372752659444103
1	25-29	0.17230478986161302
1	30-34	0.2786286777908913
1	35-39	0.43368826430388907
1	40-44	0.6248590808461536
1	45-49	0.8714202776336712
1	50-54	1.2390862602253527
1	55-59	1.5976170426610459
1	60-64	2.162776772543033
1	65-69	2.61264934246692
1	70-74	3.1449949668647044
1	75-79	1.4038408549916426
1	80-84	-0.011484956215319642
1	85-89	-0.014801184407183143
1	90-94	-0.027031028534821644
1	95-99	-0.02564955558566595
1	100-104	0.009838086254568168
1	105-109	0.10370870483755112
1	110-114	0.2508687872069828
1	115-119	0.3262302790050562
1	120-124	0.5291692082186827
1	125-129	0.5980378850577708
1	130-134	0.7574896470416554
1	135-139	1.2011554625101866
1	140-144	1.0777585518167285
1	145-149	1.7125210355966516
1	150-152	2.0547609958431714
2	1	-0.049098018348445294
2	2	-0.07549617861901936
2	3	-0.11481682442187235
2	4	-0.049676366045545706
2	5	-0.0454458573277563
2	6	-0.08106880482861811
2	7	-0.09391883936260115
2	8	-0.07371767629609849
2	9	-0.0789932922701837
2	10-14	-0.02777352090479468
2	15-19	-0.022010420996167568
2	20-24	0.015676208908956824
2	25-29	0.13011119540329474
2	30-34	0.23883382052628832
2	35-39	0.392680286367046
2	40-44	0.5816933650297038
2	45-49	0.7998675972716285
2	50-54	1.1561600968174304
2	55-59	1.4164405196391954
2	60-64	1.7155342319364593
2	65-69	1.909646085311266
2	70-74	2.009308974144176
2	75-79	0.5960971503703725
2	80-84	-0.15907149503124174
2	85-89	-0.13507469019579332
2	90-94	-0.1541826967101798
2	95-99	-0.18274614606266226
2	100-104	-0.4497050403595608
2	105-109	-0.2534254088204051
2	110-114	-0.08782308320795806
2	115-119	0.04420029778354717
2	120-124	0.03592408051326146
2	125-129	0.15624466814687565
2	130-134	0.3962361420980294
2	135-139	0.5618994851702297
2	140-144	-8.154901893707226
2	145-149	-7.40653926753798
2	150-152	-5.371066494943623
3	1	-0.12895764264803233
3	2	-0.15423960277056636
3	3	-0.21174486518913227
3	4	-0.15514697728163895
3	5	-0.13402596510201903
3	6	-0.11956668698984885
3	7	-0.1007468715821247
3	8	-0.0882370041891889
3	9	-0.08385174716631383
3	10-14	-0.04733590794278797
3	15-19	-0.05238554598411582
3	20-24	-0.011467242158886393
3	25-29	0.07949953155858935
3	30-34	0.17962301036430262
3	35-39	0.3387535059366442
3	40-44	0.5433818643195103
3	45-49	0.7436196299057976
3	50-54	0.9986917007188367
3	55-59	1.3028129333570355
3	60-64	1.6948079025356897
3	65-69	2.14497141844576
3	70-74	2.4471530321014896
3	75-79	0.730262881938625
3	80-84	-0.2030827998708986
3	85-89	-0.1594563492937695
3	90-94	-0.18480221283539322
3	95-99	-0.14484348771579647
3	100-104	-0.1622086324105254
3	105-109	-0.09480970462309557
3	110-114	-0.01048905320357818
3	115-119	0.09040935074861167
3	120-124	0.08246441329312049
3	125-129	0.24991727446165868
3	130-134	0.4233142316965264
3	135-139	0.7745759663388334
3	140-144	0.030222792345224292
3	145-149	0.6041123007902769
3	150-152	0.7821538591025217
4	1	-0.06247060095471113
4	2	-0.07601412126881257
4	3	-0.10384402380768876
4	4	-0.06411012757855161
4	5	-0.04677387867949534
4	6	-0.057463312260175314
4	7	-0.05750371714507452
4	8	-0.050238190135708294
4	9	-0.06993832584503679
4	10-14	-0.01871344722753321
4	15-19	-0.032795228161454304
4	20-24	0.027884659975313753
4	25-29	0.1457476646526814
4	30-34	0.25647504502828156
4	35-39	0.4033243802806581
4	40-44	0.5738808532941171
4	45-49	0.8013697819681553
4	50-54	1.0219770063740192
4	55-59	1.4306794874694404
4	60-64	1.781920374309756
4	65-69	2.2701887146425683
4	70-74	2.5527293729139906
4	75-79	0.9365265481234779
4	80-84	-0.1104124990808586
4	85-89	-0.061776398316851555
4	90-94	-0.03071108997908567
4	95-99	-0.028210730548430263
4	100-104	-0.07867378718518836
4	105-109	0.006866022146311934
4	110-114	0.11167478478679271
4	115-119	0.22846337902861436
4	120-124	0.33353680174649014
4	125-129	0.4673921488788011
4	130-134	0.5290873210881522
4	135-139	0.8287438646307663
4	140-144	1.079183786666718
4	145-149	1.3830265392588288
4	150-152	1.549570709267698
5	1	-0.03933047960671843
5	2	-0.054346726255978695
5	3	-0.07871214633353674
5	4	-0.040993253911743466
5	5	-0.03436329192212639
5	6	-0.08022461872293007
5	7	-0.08553327967508295
5	8	-0.07729035588212696
5	9	-0.07021435002925358
5	10-14	-0.039700295681143416
5	15-19	-0.047197065415645056
5	20-24	0.04689305715189107
5	25-29	0.12925717109860813
5	30-34	0.19452300992180227
5	35-39	0.3550533047500508
5	40-44	0.5060723916063559
5	45-49	0.6810289613293357
5	50-54	0.8283298879047436
5	55-59	1.0052736606725219
5	60-64	1.4067274487265387
5	65-69	1.9535686374849135
5	70-74	2.403337435998619
5	75-79	0.9811068744253184
5	80-84	-0.0811407593837643
5	85-89	-0.07081353588684891
5	90-94	-0.03791532191419833
5	95-99	-0.020113239860712895
5	100-104	-0.047607069869798124
5	105-109	0.09643001211303215
5	110-114	0.1688851507946083
5	115-119	0.2552601318020997
5	120-124	0.33569781066074
5	125-129	0.42016184173670723
5	130-134	0.6684585470476705
5	135-139	1.0854489884255116
5	140-144	1.2053412912751966
5	145-149	1.4925906552875539
5	150-152	1.6362621805370985
6	1	-0.06978042207556712
6	2	-0.10437436205770467
6	3	-0.08245848031629777
6	4	-0.08857419769925912
6	5	-0.09324674604960137
6	6	-0.04574849914917678
6	7	-0.10936052098269755
6	8	-0.053397758326347855
6	9	-0.027273340911790456
6	10-14	-0.03160579349481196
6	15-19	-0.01243556720832828
6	20-24	-0.03440367388412824
6	25-29	-0.029930887427916275
6	30-34	0.03701605211030312
6	35-39	0.19300646060287363
6	40-44	0.3468490216229121
6	45-49	0.5472841761030445
6	50-54	0.7094128990462565
6	55-59	0.7907531300566291
6	60-64	1.0342020898905737
6	65-69	1.3553399176254644
6	70-74	1.599853325487615
6	75-79	0.5298819997702431
6	80-84	-0.11010293600677556
6	85-89	-0.07985961786740603
6	90-94	-0.04437704016258692
6	95-99	-0.028298330601529642
6	100-104	-0.102715807012153
6	105-109	-0.008966235016075075
6	110-114	0.041332983901405385
6	115-119	0.20587328135104244
6	120-124	0.3071245512384486
6	125-129	0.4974520939278406
6	130-134	0.5282177195929947
6	135-139	0.9924603983770766
6	140-144	1.331921384565245
6	145-149	1.4965981282023186
6	150-152	1.555569428016792
7	1	-0.017656364928001267
7	2	-0.02049881085036276
7	3	-0.047390453984533565
7	4	-0.03543624238171361
7	5	-0.0034764670429510147
7	6	-0.05983929029665802
7	7	-0.040608301687427684
7	8	-0.06362767731690866
7	9	-0.07277839719486678
7	10-14	-0.04730931050128362
7	15-19	-0.054413372595199405
7	20-24	0.01122598068501901
7	25-29	0.0644354090150614
7	30-34	0.14380807278300978
7	35-39	0.22396418090279724
7	40-44	0.3646048958071155
7	45-49	0.5072680668441976
7	50-54	0.6276877753497985
7	55-59	0.8408888856141417
7	60-64	1.0164786211275398
7	65-69	1.3103604539403264
7	70-74	1.8532793531563243
7	75-79	0.8785217927223137
7	80-84	-0.04591479341398497
7	85-89	-0.04034253468268645
7	90-94	-0.024940572258870475
7	95-99	-0.11814456964045661
7	100-104	-0.047291358589767185
7	105-109	-0.026994265367441983
7	110-114	0.07770528655856879
7	115-119	0.0753603009918109
7	120-124	0.08670956605421765
7	125-129	0.2000416450911544
7	130-134	0.43207631680236247
7	135-139	0.6585100563863051
7	140-144	0.5368638012455982
7	145-149	0.7883450717792435
7	150-152	0.8693545964480336
8	1	-0.025836356427234364
8	2	-0.02630117734717885
8	3	-0.041975470312472396
8	4	-0.029970480313906478
8	5	-0.03596725016663527
8	6	-0.04831767909366391
8	7	-0.0471716704526699
8	8	-0.11279720209705602
8	9	-0.07923003439915988
8	10-14	0.002800716882155996
8	15-19	-0.021926358339747765
8	20-24	-0.025959896298836327
8	25-29	0.009168306246358782
8	30-34	0.16431755989004415
8	35-39	0.30982744652455096
8	40-44	0.4427680379762329
8	45-49	0.5370676085768409
8	50-54	0.7039768593504938
8	55-59	0.9417827990439172
8	60-64	1.2284733714090699
8	65-69	1.4685135446193378
8	70-74	1.9189625280385947
8	75-79	0.7307203630752461
8	80-84	-0.09569639987142153
8	85-89	-0.08746165783643534
8	90-94	-0.08661254867568857
8	95-99	-0.05660732787695366
8	100-104	0.004702407953274701
8	105-109	0.008161178089800103
8	110-114	-0.021734961614903625
8	115-119	0.13321912661804447
8	120-124	0.08681153145182563
8	125-129	0.10633499753888387
8	130-134	0.30806303227972975
8	135-139	0.6196836409123172
8	140-144	0.5552513120048239
8	145-149	0.7507593500348726
8	150-152	0.922378481028236
9	1	-0.09055166918876267
9	2	-0.09571170743870283
9	3	-0.10394878533276142
9	4	-0.10889867784723606
9	5	-0.09512459104937676
9	6	-0.11575438727120257
9	7	-0.08284653472828296
9	8	-0.11804272621661482
9	9	-0.07301770516195916
9	10-14	-0.014315851222960418
9	15-19	-0.015393703031804762
9	20-24	-0.004700106440822083
9	25-29	0.10919050817555132
9	30-34	0.1849556008467843
9	35-39	0.23393979546138866
9	40-44	0.3199228695862324
9	45-49	0.4800607061855544
9	50-54	0.5615521612123899
9	55-59	0.8054244853599783
9	60-64	1.2033115916225903
9	65-69	1.1657620623428109
9	70-74	1.4709409561463076
9	75-79	0.6800622411586126
9	80-84	-0.17685493812732744
9	85-89	-0.122238661230476
9	90-94	-0.14466148777077592
9	95-99	-0.12077771281372662
9	100-104	-0.1451983127991454
9	105-109	-0.014471500411204374
9	110-114	-0.0015715688837119046
9	115-119	0.04182144308399316
9	120-124	-0.001441179552784888
9	125-129	0.06482476074750565
9	130-134	0.3279657555803617
9	135-139	0.7041205272970323
9	140-144	0.782789726675869
9	145-149	0.6775852215927252
9	150-152	0.5542234208526899
10	1	-0.11364159227213833
10	2	-0.11056617816535663
10	3	-0.13023641340704017
10	4	-0.2534123366484522
10	5	-0.1419707405106294
10	6	-0.10194872135540578
10	7	-0.11577766075714635
10	8	-0.14141789858889098
10	9	-0.10415484015386767
10	10-14	-0.03974962216143041
10	15-19	-0.04487235448979021
10	20-24	-0.030845770857865773
10	25-29	0.026007434572193233
10	30-34	0.06091821920160356
10	35-39	0.11055053996228992
10	40-44	0.17542009914030032
10	45-49	0.40281249587889434
10	50-54	0.4921362343667717
10	55-59	0.6193872510278453
10	60-64	0.8839676393673948
10	65-69	1.0283234159831416
10	70-74	1.4401857513523595
10	75-79	0.574708500912493
10	80-84	-0.058455426438030145
10	85-89	-0.03253131588194691
10	90-94	-0.03613201395374688
10	95-99	0.028689248393625633
10	100-104	0.012449584691466953
10	105-109	0.08173681047824033
10	110-114	0.1462080559291863
10	115-119	0.2081945998295467
10	120-124	0.28425550545541967
10	125-129	0.355568879914788
10	130-134	0.5035633117210416
10	135-139	0.8289660913490557
10	140-144	1.0888090349322468
10	145-149	1.2377805074472725
10	150-152	1.1565491351127832
11	1	-0.016274246248080715
11	2	-0.02308466680324983
11	3	-0.029258676608407086
11	4	-0.0023366981101275996
11	5	-0.024044413330564396
11	6	-0.00976933514616718
11	7	0.013526910338462983
11	8	-0.0014039400674690228
11	9	0.007958851362808161
11	10-14	0.0375224729614132
11	15-19	0.02953025280834254
11	20-24	0.06716254022506973
11	25-29	0.11098609340159271
11	30-34	0.14094367749572
11	35-39	0.21733486800035706
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11	45-49	0.39078393853690585
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99	125-129	0.5197952099894145
99	130-134	0.7559300638664297
99	135-139	1.1723536963765113
99	140-144	1.611826362064729
99	145-149	2.001790123848238
99	150-152	2.137472130016775
100	1	-0.05229363463987369
100	2	-0.04593872790377418
100	3	-0.048312735227632686
100	4	-0.03361932775690235
100	5	-0.03514460921564222
100	6	-0.043657327382447875
100	7	-0.03330049068498653
100	8	-0.03806186649712373
100	9	-0.03814888053968701
100	10-14	-0.01513209622614653
100	15-19	-0.01888425681219985
100	20-24	0.07389531087554246
100	25-29	0.16646070728375406
100	30-34	0.24003196269759286
100	35-39	0.3434791029823785
100	40-44	0.562111045123384
100	45-49	0.924643965093999
100	50-54	1.268064184768626
100	55-59	1.6951569624530656
100	60-64	2.0947922078408006
100	65-69	2.5312631481412957
100	70-74	3.030109894712325
100	75-79	1.2555755572405367
100	80-84	-0.08959210792647099
100	85-89	-0.09167436895586434
100	90-94	-0.09148669072222049
100	95-99	-0.09041470175268529
100	100-104	-0.033549398994018986
100	105-109	0.038242143904938075
100	110-114	0.16058989637671317
100	115-119	0.2664082556192504
100	120-124	0.4943394737480489
100	125-129	0.6527278041162461
100	130-134	0.8477139661547994
100	135-139	1.2694995213962876
100	140-144	1.8921801485547363
100	145-149	2.1363030235473026
100	150-152	2.4994709494557377
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48223.0
3	13595.0
4	17662.0
5	22209.0
6	23161.0
7	29357.0
8	34038.0
9	37873.0
10	42746.0
11	49031.0
12	58409.0
13	69639.0
14	77311.0
15	84008.0
16	95564.0
17	148064.0
18	114535.0
19	141225.0
20	169229.0
21	198599.0
22	230079.0
23	271307.0
24	315979.0
25	365861.0
26	424237.0
27	494407.0
28	581263.0
29	718718.0
30	911489.0
31	1303664.0
32	2468491.0
33	1.2463097E7
34	539638.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.276745569189732	24.957076386403337	21.79713806962155	30.969039974785385
2	25.18729578027602	27.25276150362802	23.575977670765404	23.983965045330553
3	25.430724075458773	26.26349284798714	23.785719643473474	24.520063433080615
4	27.419106784522494	24.504704843053414	23.083665311805657	24.99252306061843
5	26.31690752723476	26.091810433393015	25.077699006697244	22.513583032674976
6	27.151077421244914	25.022406479375004	22.035122616250646	25.791393483129433
7	26.11915323285671	24.575882250768206	24.994250456539678	24.310714059835405
8	25.767764984187775	23.84325282355523	24.92143082262546	25.46755136963154
9	25.25211986694845	23.711360630656788	25.062086748549568	25.974432753845196
10-14	26.09847997418534	24.42657594270284	23.674408290439718	25.800535792672104
15-19	25.684996342902938	24.099282176475263	24.17446199401522	26.041259486606577
20-24	25.843945337567366	24.06422808621484	24.064624316439705	26.027202259778093
25-29	25.90987925209321	24.191258705627227	23.953660059139246	25.945201983140322
30-34	25.98807233399592	24.153008201262065	23.905405353297905	25.953514111444107
35-39	25.927570697109736	24.35229060333138	23.945676316369063	25.77446238318982
40-44	26.130731859418276	24.441441670126192	23.74845294315244	25.67937352730309
45-49	26.26181892130465	24.68082277695623	23.615728295224958	25.441630006514167
50-54	26.747649202015232	24.94612953180476	23.277382272850115	25.028838993329888
55-59	27.100901848084636	25.291269073527367	23.005320570568667	24.602508507819326
60-64	27.617176362216643	25.38330485429416	22.666964240092252	24.332554543396945
65-69	28.21316783378413	25.640920718688072	22.102271638821776	24.043639808706022
70-74	28.926383685734418	25.795124785881097	21.585769728278418	23.692721800106067
75-79	25.90403808617606	25.952881919665444	22.2821608188001	25.860919175358394
80-84	26.68947935174063	24.392333556183317	23.891067393374563	25.027119698701494
85-89	26.0610035119545	23.993590182017847	23.952225244636164	25.993181061391486
90-94	25.735450402838673	23.934361527109896	24.256213731575787	26.073974338475647
95-99	25.90452149156744	23.79911282520757	24.279343528902505	26.01702215432249
100-104	25.85529912206533	23.896023867789626	24.25737696648659	25.991300043658455
105-109	26.002927517671132	23.937094983450315	24.09654031760871	25.963437181269843
110-114	25.9915538435312	24.069094939254775	24.158589348326625	25.780761868887396
115-119	26.1697995326083	24.223573445175713	23.990890286508215	25.615736735707767
120-124	26.26894055639701	24.517232633063994	23.85775315043475	25.35607366010425
125-129	26.707769770704015	24.653117938687206	23.62695192295747	25.01216036765131
130-134	27.05044386943885	25.001296086605045	23.495914735020904	24.452345308935204
135-139	27.658255265070725	25.13004319418217	23.178045276468396	24.033656264278704
140-144	28.398285296677855	25.380553787515385	22.940082257499427	23.281078658307333
145-149	29.33813194415823	25.64865287973469	22.576195961230223	22.43701921487686
150-152	30.207984428281122	25.844434672145418	22.274741835266248	21.67283906430722
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	484.0
1	286.0
2	70.0
3	53.5
4	53.5
5	44.5
6	35.5
7	35.0
8	46.0
9	59.0
10	59.5
11	82.0
12	127.5
13	159.0
14	207.5
15	318.5
16	431.5
17	580.0
18	821.5
19	1141.0
20	1586.0
21	2241.0
22	3127.0
23	4291.5
24	5736.0
25	7713.0
26	10209.0
27	13359.0
28	17480.0
29	22760.0
30	30020.0
31	39504.5
32	51999.0
33	69086.5
34	92881.0
35	125328.0
36	170115.5
37	228855.5
38	300235.0
39	380983.0
40	462374.5
41	538760.5
42	606487.0
43	665699.0
44	716847.0
45	761846.5
46	803609.0
47	839504.5
48	870511.0
49	898015.0
50	918509.5
51	932190.0
52	941989.5
53	949091.5
54	953973.0
55	955314.0
56	948666.0
57	931812.0
58	893427.0
59	822593.5
60	734576.0
61	642739.5
62	551157.5
63	465524.5
64	389093.5
65	322574.5
66	266886.5
67	220182.0
68	180341.5
69	147425.0
70	119884.5
71	97160.0
72	79387.0
73	65104.5
74	53198.5
75	43724.5
76	36168.0
77	29951.5
78	24852.5
79	20656.5
80	16949.0
81	13865.0
82	11219.5
83	9017.0
84	7210.5
85	5603.0
86	4310.5
87	3218.5
88	2293.0
89	1654.0
90	1150.0
91	717.0
92	459.5
93	283.0
94	144.0
95	76.5
96	36.0
97	15.5
98	12.5
99	33.5
100	147.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4412059048940402
2	0.0
3	0.08259203638144855
4	0.0
5	0.0
6	1.6841949999973408E-4
7	2.083083289470395E-4
8	4.742338552624091E-4
9	0.001440429934208252
10-14	1.1257513947350647E-4
15-19	1.5955531578922176E-5
20-24	3.004958447363676E-4
25-29	0.012104043539454573
30-34	9.085788815775129E-4
35-39	0.0063157312499900285
40-44	0.02303890118154257
45-49	0.015795089844711903
50-54	0.020868062468388102
55-59	0.004398408205256213
60-64	0.00688569829472597
65-69	0.006445148339463508
70-74	0.019193618071022326
75-79	0.39464234523621894
80-84	0.6330906733358425
85-89	0.5684104939885761
90-94	0.5474812686491356
95-99	0.6255951191674333
100-104	0.7850316548882342
105-109	0.6654174667331598
110-114	0.6891823446015434
115-119	0.6522364248121281
120-124	0.7833722796040263
125-129	1.0335736295483682
130-134	0.831726404472371
135-139	0.6394276786279377
140-144	0.756481890383016
145-149	0.7020017278067863
150-152	0.7897796074241915
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
152	2.2562708E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.26984186450518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.0367757965553	95.36021678636884
2	1.7351995646000873	3.375651745040175
3	0.12332287075463531	0.35986788409740567
4	0.03821520753543251	0.1486874877516307
5	0.01739030221826371	0.08457759733732324
6	0.012795019421390713	0.07467417094631684
7	0.006238406147066614	0.042476614558820854
8	0.0072942666218099505	0.056760972865679396
9	0.005685879422203434	0.049775813303253494
>10	0.016826223409415673	0.24544275446318292
>50	1.678775634328394E-4	0.010320296879160244
>100	6.490661936362411E-5	0.012614360687484137
>500	1.0006885832642047E-5	0.005313557175886539
>1k	9.115445505136616E-6	0.025077193553122507
>5k	0.0	0.0
>10k+	4.556800205901066E-6	0.148542764971714
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	33513	0.14853270272344968	Illumina Paired End PCR Primer 2 (100% over 31bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.534556578935472E-5	4.43209210525616E-6	4.43209210525616E-6	0.0
2	1.994441447365272E-4	4.43209210525616E-6	8.86418421051232E-6	0.0
3	4.343450263151037E-4	8.86418421051232E-6	1.772836842102464E-5	0.0
4	6.426533552621431E-4	8.86418421051232E-6	2.21604605262808E-5	0.0
5	9.263072499985374E-4	8.86418421051232E-6	2.21604605262808E-5	0.0
6	0.0011301834868403208	8.86418421051232E-6	2.659255263153696E-5	0.0
7	0.0013562201842083849	8.86418421051232E-6	4.43209210525616E-5	0.0
8	0.0015556643289449121	8.86418421051232E-6	5.761719736833008E-5	0.0
9	0.0017684047499972077	8.86418421051232E-6	6.204928947358624E-5	4.43209210525616E-6
10-14	0.0029686152921005755	1.3296276315768481E-5	7.623198421040595E-5	1.0637021052614784E-5
15-19	0.014029344349977848	1.3296276315768481E-5	1.0902946578930154E-4	2.1274042105229568E-5
20-24	0.17895281009708586	1.5069113157870943E-5	1.3030350789453108E-4	2.7478971052588196E-5
25-29	0.20711255049704141	2.21604605262808E-5	1.5069113157870943E-4	4.077524736835667E-5
30-34	0.3108456662205618	2.21604605262808E-5	1.8614786842075873E-4	5.673077894727885E-5
35-39	0.6979410450199507	2.8365389473639422E-5	2.1362683947334692E-4	6.64813815788424E-5
40-44	1.6131600869895582	3.545673684204928E-5	2.597205973680109E-4	7.977765789461088E-5
45-49	3.2942083015921675	3.545673684204928E-5	3.004958447363676E-4	8.686900526302073E-5
50-54	5.786449924361916	3.634315526310051E-5	3.412710921047243E-4	8.952826052617443E-5
55-59	8.95350682196481	4.43209210525616E-5	3.90024105262542E-4	9.396035263143058E-5
60-64	12.735004149324627	5.229868684202268E-5	4.077524736835667E-4	1.0193811842089168E-4
65-69	15.37124355817573	5.495794210517639E-5	4.4498204736771843E-4	1.409405289471459E-4
70-74	15.455289320767704	6.293570789463747E-5	5.123498473676121E-4	1.6487382631552914E-4
75-79	15.4555419500177	6.914063684199608E-5	5.291917973675854E-4	1.8260219473655378E-4
80-84	15.455580066009807	7.179989210514978E-5	5.646485342096347E-4	1.9323921578916857E-4
85-89	15.45559336228612	7.534556578935472E-5	5.841497394727618E-4	2.0210339999968087E-4
90-94	15.45574937192823	8.066407631566211E-5	5.956731789464279E-4	2.1451325789439814E-4
95-99	15.456651745880857	9.218751578932812E-5	6.178336394727088E-4	2.3401446315752524E-4
100-104	15.457232349946645	1.0016528157878922E-4	6.541767947358092E-4	2.490835763153962E-4
105-109	15.459178038380855	1.0725662894719907E-4	6.860878578936536E-4	2.71244036841677E-4
110-114	15.467737294654523	1.1878006842086508E-4	7.126804105251905E-4	2.863131499995479E-4
115-119	15.496019360796586	1.2321216052612124E-4	7.339544526304201E-4	2.996094263153164E-4
120-124	15.555108899162281	1.2853067105242865E-4	7.552284947356495E-4	3.102464473679312E-4
125-129	15.660417180420009	1.3118992631558232E-4	7.782753736829816E-4	3.191106315784435E-4
130-134	15.817689968775023	1.3296276315768478E-4	8.243691315776456E-4	3.244291421047509E-4
135-139	16.043330437108878	1.3296276315768478E-4	8.59825868419695E-4	3.2797481578895584E-4
140	16.20596251123757	1.3296276315768478E-4	8.775542368407197E-4	3.2797481578895584E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCA	4930	0.0	30.488749	145-146
ATCGGAC	10775	0.0	22.976433	70-74
CGGTTCG	4920	0.0	22.902122	70-74
GATCGGT	8785	0.0	21.609955	70-74
TCGGAAC	10910	0.0	21.540104	70-74
ATCGGAT	7485	0.0	21.165277	70-74
GATCGGC	12890	0.0	19.739326	70-74
TCGGAAT	8610	0.0	19.435192	70-74
AGCGGTA	7255	0.0	19.016083	70-74
GCGGTTA	6605	0.0	18.82969	70-74
CGCCGTA	3380	0.0	18.668472	140-144
AGCGGTC	10700	0.0	18.343353	70-74
ATCGGAG	12550	0.0	18.131401	70-74
TCGGAAA	12275	0.0	18.001835	70-74
GCGTCGG	7725	0.0	17.132132	145-146
TAGATCT	25225	0.0	16.98971	145-146
CGGTTCC	13090	0.0	16.981499	70-74
GCGGTTG	9560	0.0	15.806988	70-74
TATGCCG	5960	0.0	15.490955	60-64
CGGAAGT	11465	0.0	15.436775	70-74
>>END_MODULE
