FastQCFastQC Report
Sun 14 Aug 2016
C91_H3K4me1_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameC91_H3K4me1_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22562708
Sequences flagged as poor quality0
Sequence length152
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG335130.14853270272344968Illumina Paired End PCR Primer 2 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA49300.030.488749145-146
ATCGGAC107750.022.97643370-74
CGGTTCG49200.022.90212270-74
GATCGGT87850.021.60995570-74
TCGGAAC109100.021.54010470-74
ATCGGAT74850.021.16527770-74
GATCGGC128900.019.73932670-74
TCGGAAT86100.019.43519270-74
AGCGGTA72550.019.01608370-74
GCGGTTA66050.018.8296970-74
CGCCGTA33800.018.668472140-144
AGCGGTC107000.018.34335370-74
ATCGGAG125500.018.13140170-74
TCGGAAA122750.018.00183570-74
GCGTCGG77250.017.132132145-146
TAGATCT252250.016.98971145-146
CGGTTCC130900.016.98149970-74
GCGGTTG95600.015.80698870-74
TATGCCG59600.015.49095560-64
CGGAAGT114650.015.43677570-74
Produced by FastQC (version 0.11.3)