FastQCFastQC Report
Sun 14 Aug 2016
C104_input.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameC104_input.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37798758
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC57300.020.22958611
AGCGGTT73100.015.91863710
GAGCGGT75550.015.422189
ATGCCGA76350.014.97481525
CGGTTCA78250.014.85206812
AATGCCG80350.014.3407724
AGAGCGG83400.014.0783618
AAGAGCG86100.013.7588647
TGCCGAG84400.013.61780226
TCGGAAG87000.013.3583253
ATCGGAA93000.012.4803572
CGGAAGA94750.012.4239094
GATCGGA96000.012.0349251
GTTCAGC127800.09.45791814
GAATGCC129200.09.301187523
GGTTCAG135150.09.08778613
ACCGATC8600.08.54731722
GACCGAT8650.08.32277621
CCGAGAC32450.08.27689928
GAGACCG32650.08.1342130
Produced by FastQC (version 0.11.3)