##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	C104_H3K4me1_ChIPSeq.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16268649
Sequences flagged as poor quality	0
Sequence length	152
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.497218914735946	34.0	34.0	34.0	34.0	34.0
2	33.45657306885163	34.0	34.0	34.0	34.0	34.0
3	33.42771215974971	34.0	34.0	34.0	34.0	34.0
4	33.43576119934728	34.0	34.0	34.0	34.0	34.0
5	33.43274294011752	34.0	34.0	34.0	34.0	34.0
6	33.49991028757213	34.0	34.0	34.0	34.0	34.0
7	33.479662017417674	34.0	34.0	34.0	34.0	34.0
8	33.46478899384946	34.0	34.0	34.0	34.0	34.0
9	33.46543514461465	34.0	34.0	34.0	34.0	34.0
10-14	33.52804335504442	34.0	34.0	34.0	34.0	34.0
15-19	33.50604407286677	34.0	34.0	34.0	34.0	34.0
20-24	33.385115309820755	34.0	34.0	34.0	34.0	34.0
25-29	33.24066619176553	34.0	34.0	34.0	33.0	34.0
30-34	33.12926924663505	34.0	34.0	34.0	33.0	34.0
35-39	32.96491379216554	34.0	34.0	34.0	33.0	34.0
40-44	32.73332245351166	34.0	34.0	34.0	32.2	34.0
45-49	32.34594746005031	34.0	34.0	34.0	31.2	34.0
50-54	32.036353897610056	34.0	33.8	34.0	30.6	34.0
55-59	31.631669870067263	34.0	33.0	34.0	29.2	34.0
60-64	31.108342518177142	34.0	33.0	34.0	27.0	34.0
65-69	30.454670046664596	34.0	32.0	34.0	23.4	34.0
70-74	29.715555753892044	34.0	31.4	34.0	9.8	34.0
75-79	31.528625431650777	33.6	32.6	34.0	21.2	34.0
80-84	33.34876904652624	34.0	34.0	34.0	34.0	34.0
85-89	33.2897164355811	34.0	34.0	34.0	34.0	34.0
90-94	33.2189205508091	34.0	34.0	34.0	34.0	34.0
95-99	33.07963680327727	34.0	34.0	34.0	33.0	34.0
100-104	32.94068672819728	34.0	34.0	34.0	33.0	34.0
105-109	32.877789680015844	34.0	34.0	34.0	33.0	34.0
110-114	32.72370010564491	34.0	34.0	34.0	32.6	34.0
115-119	32.53485763937743	34.0	34.0	34.0	32.0	34.0
120-124	32.13511345656299	34.0	33.8	34.0	30.8	34.0
125-129	31.734675227180823	34.0	33.0	34.0	29.6	34.0
130-134	31.537697002375552	34.0	33.0	34.0	29.0	34.0
135-139	31.123083717646132	34.0	33.0	34.0	27.4	34.0
140-144	30.070538653824297	34.0	31.8	34.0	17.8	34.8
145-149	29.299850208828037	33.6	31.4	34.0	6.4	34.4
150-152	28.07802350397996	33.0	29.333333333333332	34.0	2.0	34.666666666666664
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.03362581712654844
1	2	-0.048909552517628185
1	3	-0.0378862682566421
1	4	-0.020780659857770445
1	5	-0.03257210885787032
1	6	-0.04448060283084487
1	7	-0.03170194113067737
1	8	-0.029621608850554537
1	9	-0.031156698901142477
1	10-14	-0.04836463713951389
1	15-19	-0.04433251622259604
1	20-24	-0.010202737333372625
1	25-29	0.020125578584426762
1	30-34	0.07359542547682452
1	35-39	0.1386469188727233
1	40-44	0.21352418666061368
1	45-49	0.2950117859657908
1	50-54	0.42494559624854844
1	55-59	0.6083017750338264
1	60-64	0.8518751844907371
1	65-69	0.9861846042531894
1	70-74	1.2693925251752773
1	75-79	0.4828558059141912
1	80-84	-0.08766818385192465
1	85-89	-0.09699546627062716
1	90-94	-0.08278048253418291
1	95-99	-0.08312184601069816
1	100-104	-0.08833375050154757
1	105-109	-0.02952412582883568
1	110-114	-0.011500239784247412
1	115-119	0.052971255629479685
1	120-124	0.061333994048652585
1	125-129	0.10743202773539551
1	130-134	0.23628092470145745
1	135-139	0.4227042100724354
1	140-144	0.8665051319229775
1	145-149	0.9989160661301781
1	150-152	0.8947132093002601
2	1	-0.10268321080412335
2	2	-0.1357895862777454
2	3	-0.1118308529574179
2	4	-0.1416072502880965
2	5	-0.10691050571573157
2	6	-0.12495810537098606
2	7	-0.1217960681619914
2	8	-0.1379834001678688
2	9	-0.15620484207006768
2	10-14	-0.1059853159998525
2	15-19	-0.09457704339116191
2	20-24	-0.10729881710742717
2	25-29	-0.10220214400285244
2	30-34	-0.03715323370720114
2	35-39	0.0061090681518010115
2	40-44	0.06256292550727238
2	45-49	0.01557444011833553
2	50-54	0.15985361619897276
2	55-59	0.19758405802228296
2	60-64	0.2631739541297051
2	65-69	0.4244737255386788
2	70-74	0.4427835248109524
2	75-79	0.13050624672330713
2	80-84	-0.11191272975419508
2	85-89	-0.126840162831364
2	90-94	-0.10544156063945564
2	95-99	-0.09883113235989072
2	100-104	-0.16361584319495392
2	105-109	-0.12204240869700556
2	110-114	-0.07167935968442407
2	115-119	-0.08216601168428639
2	120-124	-0.21042895550490925
2	125-129	-0.18299521028969679
2	130-134	0.005937706378311702
2	135-139	0.13374338965426702
2	140-144	0.4127741963072822
2	145-149	0.3744932502465552
2	150-152	0.4882460566417244
3	1	-0.057334127002526714
3	2	-0.06520456932162233
3	3	-0.06185022069364976
3	4	-0.08018629303585811
3	5	-0.053723054829177386
3	6	-0.056944137300369846
3	7	-0.05058375016218264
3	8	-0.057618606308629694
3	9	-0.046788938695058846
3	10-14	-0.05893858326435719
3	15-19	-0.0504158708807978
3	20-24	-0.031021220026111962
3	25-29	-0.0010662258409084302
3	30-34	0.0038768967223745676
3	35-39	0.009780549048272746
3	40-44	0.07304360935368948
3	45-49	0.09920352215153372
3	50-54	0.20404159229065755
3	55-59	0.3298962953948106
3	60-64	0.45304883718535294
3	65-69	0.5721519345498614
3	70-74	0.5492517980824516
3	75-79	0.1932990732257167
3	80-84	-0.09100458796676492
3	85-89	-0.1064125978194923
3	90-94	-0.07205320205240895
3	95-99	-0.05916089611562825
3	100-104	-0.04266380833460204
3	105-109	-0.04329298128032377
3	110-114	0.007723603128219736
3	115-119	-0.0022899484580634066
3	120-124	-0.06435657353411983
3	125-129	-0.0060825840666360875
3	130-134	0.10264013789899451
3	135-139	0.21766285688137543
3	140-144	0.5551952900682195
3	145-149	0.7381891609661508
3	150-152	0.8118834841352047
4	1	-0.09578680131140516
4	2	-0.0887360693861794
4	3	-0.08985790537617788
4	4	-0.09846526474527906
4	5	-0.09474317617683425
4	6	-0.08893613576587711
4	7	-0.09926044770319464
4	8	-0.08147523486089625
4	9	-0.08712051789557762
4	10-14	-0.09384981478452659
4	15-19	-0.10314990356722831
4	20-24	-0.09025831580515131
4	25-29	-0.09829432629110357
4	30-34	-0.02415316147985891
4	35-39	0.02876875920733113
4	40-44	0.07671527683665147
4	45-49	0.1277606700940197
4	50-54	0.23067467928952112
4	55-59	0.34090672168339964
4	60-64	0.38775351270118463
4	65-69	0.5379690853931471
4	70-74	0.7180356871988245
4	75-79	0.25617359761910663
4	80-84	-0.14915226743252674
4	85-89	-0.12077484374798075
4	90-94	-0.11469706751564246
4	95-99	-0.13952328485324728
4	100-104	-0.08418922755058844
4	105-109	-0.07148502041868454
4	110-114	-0.04874061904018845
4	115-119	-0.028298178428741494
4	120-124	-0.040701444767265116
4	125-129	-0.029838482645651254
4	130-134	0.11852820395548136
4	135-139	0.23070447619620538
4	140-144	0.5526008389029613
4	145-149	0.6941213673157591
4	150-152	0.689897137789842
5	1	-0.014758035304552664
5	2	-0.03542125902357185
5	3	-0.03454751254817978
5	4	-0.01626008887592434
5	5	-0.016973814157935863
5	6	0.0033691096028007905
5	7	-0.008816056286832463
5	8	-0.021383044032660337
5	9	-0.001510301745383913
5	10-14	-0.00922892928166874
5	15-19	-0.03364811732281936
5	20-24	-0.06531651943593175
5	25-29	-0.05225443393379692
5	30-34	-0.06314337600599629
5	35-39	-0.015539491063755406
5	40-44	0.04992868436082176
5	45-49	0.10194052151877031
5	50-54	0.12044284281404316
5	55-59	0.1573696301932408
5	60-64	0.11133147366657781
5	65-69	0.28119126778620185
5	70-74	0.5988440974589828
5	75-79	0.08061055869571732
5	80-84	-0.11847447708838388
5	85-89	-0.1015542120859223
5	90-94	-0.15824615955586552
5	95-99	-0.15864370572951003
5	100-104	-0.17850515826005164
5	105-109	-0.1367264476882255
5	110-114	-0.09182002374237896
5	115-119	-0.08591651268989153
5	120-124	-0.20339221374233674
5	125-129	-0.15056676638087652
5	130-134	-0.08743405688625927
5	135-139	0.06295244492936192
5	140-144	0.16375967429010174
5	145-149	0.2541774057439561
5	150-152	0.17591190694582792
6	1	-0.08554880154953537
6	2	-0.08628021095971405
6	3	-0.10478499122679352
6	4	-0.11757373450798525
6	5	-0.11442443642448552
6	6	-0.08647908054361153
6	7	-0.0799297516799129
6	8	-0.12538173496781724
6	9	-0.09180626097927558
6	10-14	-0.10940671543158942
6	15-19	-0.1067770941314592
6	20-24	-0.08653577070213458
6	25-29	-0.08960572846832093
6	30-34	-0.03389452240597279
6	35-39	0.003176892866981973
6	40-44	0.005695110974393458
6	45-49	0.009020298617159028
6	50-54	0.08758221227803631
6	55-59	0.18205181203385834
6	60-64	0.31447736802149606
6	65-69	0.2666162704705748
6	70-74	0.4113813868427094
6	75-79	0.20319922440046412
6	80-84	-0.11491373591890408
6	85-89	-0.10724546362776977
6	90-94	-0.0788962146856278
6	95-99	-0.04969658476401406
6	100-104	-0.06725806031870718
6	105-109	-0.04466622843522572
6	110-114	-0.0013327062595678285
6	115-119	0.05693943956195113
6	120-124	0.12802772095667336
6	125-129	0.08690715181766251
6	130-134	0.15281779920261584
6	135-139	0.30006938854474896
6	140-144	0.6449576555897885
6	145-149	0.6517463747104877
6	150-152	0.41422245005949776
7	1	-0.07496881052328774
7	2	-0.09130517069059607
7	3	-0.07698201433895235
7	4	-0.09002084456820114
7	5	-0.08878870664836569
7	6	-0.08194620824270515
7	7	-0.1083630648935241
7	8	-0.07484762758904395
7	9	-0.07507335947806837
7	10-14	-0.08353338450788073
7	15-19	-0.06934010179831773
7	20-24	-0.08619731870992098
7	25-29	-0.08589078366993874
7	30-34	-0.004537986998236931
7	35-39	-0.00417933570017226
7	40-44	-0.026779335792625147
7	45-49	-0.05480878998914562
7	50-54	-0.003305386436210256
7	55-59	0.03606024362146698
7	60-64	0.06565177514256959
7	65-69	0.26747956378999405
7	70-74	0.39116030285860504
7	75-79	0.11245396974651811
7	80-84	-0.1040439897478791
7	85-89	-0.07684190148750503
7	90-94	-0.0625846922577793
7	95-99	-0.04967298563670397
7	100-104	-0.07965720205532278
7	105-109	-0.07718710932291373
7	110-114	-0.08037114679719082
7	115-119	-0.08529030480482191
7	120-124	-0.0939874531115521
7	125-129	-0.031196865657413753
7	130-134	0.02708771001111998
7	135-139	0.15316367852038226
7	140-144	0.2373468072022682
7	145-149	0.12946036337697464
7	150-152	-0.10982483316484704
8	1	-0.028560764425222374
8	2	-0.046015828163668004
8	3	-0.04798547534579001
8	4	-0.05741867406516121
8	5	-0.0307054078486928
8	6	-0.029838113821028855
8	7	-0.010364525812228464
8	8	-0.03327616895400354
8	9	-0.019655850034531852
8	10-14	-0.0047749260359282175
8	15-19	0.016165377895397626
8	20-24	0.011481165394449988
8	25-29	0.026472727319145406
8	30-34	0.05594780580884162
8	35-39	0.11124530593501447
8	40-44	0.12208407364856555
8	45-49	0.16445670593465422
8	50-54	0.16599557537288234
8	55-59	0.280452428593982
8	60-64	0.24482960525178044
8	65-69	0.29337240258509567
8	70-74	0.33688109361142793
8	75-79	0.08743575343579124
8	80-84	-0.03532591367614657
8	85-89	-0.0010682803458834655
8	90-94	-0.0011587649413229428
8	95-99	-0.01891226852798411
8	100-104	-0.030947447494206415
8	105-109	-0.012748520811449282
8	110-114	0.009186953161481881
8	115-119	0.04798296771793531
8	120-124	0.004168903872226792
8	125-129	0.04071399448714175
8	130-134	0.1273677302000351
8	135-139	0.17355735656395055
8	140-144	0.18664515587985875
8	145-149	0.0020501734627877966
8	150-152	-0.21012760272770947
9	1	-0.03453270904515193
9	2	-0.03871788825292555
9	3	-0.051964304948697304
9	4	-0.039092295866417714
9	5	-0.04135653596834743
9	6	-0.04903129123615457
9	7	-0.05553638135861405
9	8	-0.06666830352436648
9	9	-0.058099195639201184
9	10-14	-0.05486107650822447
9	15-19	-0.07175595928958245
9	20-24	-0.0912195809488594
9	25-29	-0.08402123143343232
9	30-34	-0.04323903217468228
9	35-39	-0.03658629065368757
9	40-44	9.496448880952357E-4
9	45-49	0.024469424036389853
9	50-54	0.09181287293935014
9	55-59	0.044816933292121774
9	60-64	0.078030267220349
9	65-69	0.1871040053005011
9	70-74	0.14081188614836648
9	75-79	0.01100200859612599
9	80-84	-0.09068444303103718
9	85-89	-0.07190063275783132
9	90-94	-0.07561389676266828
9	95-99	-0.08603644313043901
9	100-104	-0.0683093176761318
9	105-109	-0.06699377322055255
9	110-114	-0.045384538308766764
9	115-119	-0.02348089899425787
9	120-124	-0.002982868035815045
9	125-129	-0.11491434191413319
9	130-134	-0.02622467847023202
9	135-139	0.18576205881890218
9	140-144	0.41864658543713773
9	145-149	0.287924890920241
9	150-152	-0.07374968819985739
10	1	-0.01498737334780742
10	2	-0.026086588425854984
10	3	-0.03876134064859116
10	4	-0.012579000838705667
10	5	-0.009079714336600375
10	6	-0.03055657105402787
10	7	-0.02984592327628377
10	8	-0.026213398366294882
10	9	-0.02556762829377135
10	10-14	-0.039630271359193614
10	15-19	-0.027846373353362708
10	20-24	-0.024881877842538813
10	25-29	0.029621796824038427
10	30-34	0.025796558821518545
10	35-39	0.04040494215168877
10	40-44	0.042664356508382184
10	45-49	0.09054803060167416
10	50-54	0.16209453439790167
10	55-59	0.22127570069094915
10	60-64	0.27766370747649916
10	65-69	0.37700375886087656
10	70-74	0.32328292173667705
10	75-79	0.12430493127710207
10	80-84	-0.03186453514872056
10	85-89	-0.012504706868114113
10	90-94	-0.001889364494637391
10	95-99	-0.0021466876008133795
10	100-104	0.008522670380905595
10	105-109	-0.004229140904733697
10	110-114	-0.005187907440188155
10	115-119	-0.013679975807427525
10	120-124	-0.034082038655711244
10	125-129	-0.09993438236708485
10	130-134	0.026090421035558364
10	135-139	0.1755691476742527
10	140-144	0.3226749558634374
10	145-149	0.15150583901600712
10	150-152	0.058082457647135044
11	1	-0.06324725269941212
11	2	-0.08356616130318884
11	3	-0.06902994651197503
11	4	-0.07427198018117309
11	5	-0.06378067249348618
11	6	-0.0866572449900147
11	7	-0.20825348777133712
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11	9	-0.08069806569569948
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98	40-44	0.22032662222285637
98	45-49	0.31638880033904826
98	50-54	0.5153343878172976
98	55-59	0.7211920461603079
98	60-64	0.9202985805785637
98	65-69	1.1385993428959615
98	70-74	1.4415657880753763
98	75-79	0.6278553308105046
98	80-84	-0.05898031531865655
98	85-89	-0.03850471686661194
98	90-94	-0.016181886211363405
98	95-99	-0.020608141358081866
98	100-104	0.01712209157359723
98	105-109	0.05579581003377854
98	110-114	0.05351133791302232
98	115-119	0.09932452356238741
98	120-124	0.1753649156235184
98	125-129	0.16199988060676063
98	130-134	0.32950302094185346
98	135-139	0.5492771307165114
98	140-144	0.9205425630985253
98	145-149	1.195968200259042
98	150-152	1.39118630764672
99	1	-0.045610551356951134
99	2	-0.048490470887244896
99	3	-0.0375537133340913
99	4	-0.04568647021441308
99	5	-0.07067988239946743
99	6	-0.013719224025912524
99	7	0.0022169548637549497
99	8	-0.010556554841642196
99	9	-0.0150339794847838
99	10-14	0.0014870033878651157
99	15-19	-0.005641812851393979
99	20-24	0.019791122624411628
99	25-29	0.0575277804303127
99	30-34	0.07863912795911432
99	35-39	0.15439406235719133
99	40-44	0.22331994834477342
99	45-49	0.37961926433168713
99	50-54	0.4643596846112672
99	55-59	0.6025588405899747
99	60-64	0.8097358557464389
99	65-69	0.9720594284322033
99	70-74	1.3116370741187033
99	75-79	0.594678805190064
99	80-84	-0.007871811459423839
99	85-89	-0.00398397429078301
99	90-94	-0.004338785307865578
99	95-99	-0.01635625622640191
99	100-104	-0.04052738575744286
99	105-109	0.01516587377371792
99	110-114	0.10329600949720685
99	115-119	0.14514921409938353
99	120-124	0.15254137605835183
99	125-129	0.11208008815480142
99	130-134	0.23762203632633572
99	135-139	0.4804150542167491
99	140-144	1.0106287043922428
99	145-149	1.2633310918817173
99	150-152	1.4612783238953142
100	1	-0.036196873949577935
100	2	-0.013969464900370099
100	3	-0.006712408578849249
100	4	-0.027268342487182906
100	5	-0.025489692877819436
100	6	-0.018303105787204288
100	7	-0.03213518825683792
100	8	-0.01886686345013544
100	9	-0.04023803168325912
100	10-14	-0.026181820248041276
100	15-19	-0.015220533425583938
100	20-24	0.025859248508133703
100	25-29	0.0855909210576371
100	30-34	0.10456491326521444
100	35-39	0.14345487557044123
100	40-44	0.21059811011248541
100	45-49	0.3591372766897081
100	50-54	0.5103187066610388
100	55-59	0.6787174987093856
100	60-64	0.9093231381119189
100	65-69	1.1952598288244793
100	70-74	1.4156868409499062
100	75-79	0.6250773743719762
100	80-84	-0.05915184454817535
100	85-89	-0.06392174151186225
100	90-94	-0.0594253185963467
100	95-99	-0.06856053155962627
100	100-104	-0.05285808880541509
100	105-109	-0.0076888680565758705
100	110-114	0.04027953133411444
100	115-119	0.08618434117327922
100	120-124	0.18383247020067728
100	125-129	0.2271934614023401
100	130-134	0.3524942377809097
100	135-139	0.5819875111982498
100	140-144	1.1056816194774193
100	145-149	1.3318303556901405
100	150-152	1.4874740180453188
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	18909.0
3	5360.0
4	6575.0
5	7881.0
6	8302.0
7	9979.0
8	11451.0
9	13330.0
10	15294.0
11	18246.0
12	22196.0
13	27444.0
14	31967.0
15	39734.0
16	45523.0
17	94535.0
18	49215.0
19	56986.0
20	70669.0
21	82914.0
22	95430.0
23	112442.0
24	134459.0
25	155391.0
26	182548.0
27	217417.0
28	256538.0
29	327784.0
30	437634.0
31	644929.0
32	1276703.0
33	1.0813965E7
34	976899.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.794387718976395	24.11493860036653	22.024391249429897	30.066282431227176
2	26.123561697558014	26.56009751473878	22.636088198466837	24.680252589236375
3	26.51206590621351	25.52990447442356	23.2171735171957	24.74085610216723
4	28.871622960210157	23.496026006830682	22.303265624576447	25.329085408382713
5	27.302820289502833	25.41136636484074	24.245215444748975	23.040597900907443
6	28.341609681295598	24.23489498113826	20.927945522704437	26.4955498148617
7	26.84583766426552	24.083183402658985	24.11986749959892	24.951111433476576
8	27.26737844485512	23.14060574805093	23.727735161717526	25.864280645376432
9	26.447326913937097	23.048358397139225	23.752966755860918	26.751347933062757
10-14	27.08101658094081	23.74501975966944	22.92526114010274	26.248702519287008
15-19	26.475235896969686	23.384949789008296	23.548303242635573	26.591511071386442
20-24	26.50308483762326	23.51716594606578	23.395522325215083	26.584226891095874
25-29	26.631049433071475	23.73829389704623	23.201972424353066	26.428684245529233
30-34	26.670226349715957	23.727822895126046	23.189589769907094	26.4123609852509
35-39	26.53699421840956	24.056014209477926	23.304190462081575	26.102801110030942
40-44	26.8778120928364	24.34397672598879	22.930144312154127	25.84806686902068
45-49	27.125940994286758	24.782287442904877	22.69280306581532	25.398968496993053
50-54	27.82226110720241	25.1930682583237	22.239337673231855	24.745332961242035
55-59	28.26524649958152	25.63866992869213	21.90212824553612	24.193955326190235
60-64	28.86062652391057	25.818489094110873	21.516269213404758	23.804615168573793
65-69	29.501008598607875	26.129585628421715	20.792756034653298	23.57664973831711
70-74	30.018018122129575	26.3596680760946	20.328235698084825	23.294078103691
75-79	27.25709387784721	25.735228363325017	21.55866027020221	25.449017488625557
80-84	27.75956983282377	23.808866758061097	22.96868306192662	25.46288034718851
85-89	26.933290730352937	23.26366598276048	23.237373575624826	26.56566971126176
90-94	26.31306427229891	23.231422715823317	23.749555225249182	26.70595778662859
95-99	26.4270006148491	23.11594640754511	23.81156970175688	26.645483275848914
100-104	26.385371213805904	23.26706716013522	23.696377280000927	26.65118434605795
105-109	26.563896562068983	23.323219943805277	23.557756045416	26.555127448709737
110-114	26.521845298649744	23.532249370561665	23.696989334132454	26.24891599665614
115-119	26.841361778519694	23.86167389118111	23.388203309285	25.908761021014197
120-124	26.974802765783274	24.523411913003727	23.080676539571602	25.421108781641394
125-129	27.658589722155202	24.60622665053788	22.87814433863828	24.857039288668634
130-134	28.219319105829133	25.039841669254642	22.668553022488997	24.072286202427232
135-139	29.196232592164684	25.23452443626992	22.264889492680624	23.304353478884778
140-144	30.09346477161276	25.599946065983648	22.039653026803062	22.266936135600524
145-149	31.091844408957943	26.055747735659306	21.81017690785756	21.04223094752519
150-152	31.86142247873655	26.270477457724244	21.660736017823716	20.20736404571549
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	489.0
1	290.5
2	71.5
3	40.5
4	26.5
5	27.5
6	30.0
7	30.0
8	35.0
9	41.0
10	46.5
11	57.5
12	85.5
13	117.5
14	160.5
15	254.0
16	371.0
17	560.0
18	849.0
19	1194.5
20	1657.0
21	2311.5
22	3214.0
23	4468.5
24	5985.0
25	7875.5
26	10261.0
27	12990.5
28	16267.0
29	20305.5
30	25192.0
31	30934.5
32	37486.0
33	45336.0
34	55260.5
35	67712.5
36	84204.0
37	107315.5
38	137325.0
39	175196.5
40	220413.5
41	270404.5
42	321703.5
43	371100.5
44	417988.0
45	460751.5
46	500689.0
47	538908.5
48	576212.5
49	611209.0
50	642307.5
51	671252.0
52	699186.5
53	726285.5
54	752883.0
55	776124.0
56	791451.5
57	804240.0
58	790320.5
59	728260.5
60	646315.5
61	563472.5
62	480438.0
63	400931.0
64	329299.0
65	267757.5
66	216329.5
67	173855.5
68	138166.5
69	109012.0
70	86206.5
71	68024.5
72	53962.0
73	42613.0
74	33589.5
75	26829.0
76	21525.0
77	17523.0
78	14239.0
79	11504.5
80	9253.0
81	7410.0
82	5770.5
83	4433.5
84	3427.5
85	2586.5
86	1949.5
87	1399.5
88	945.5
89	657.5
90	428.5
91	277.0
92	170.0
93	98.0
94	53.5
95	30.0
96	21.0
97	15.0
98	20.5
99	34.5
100	287.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2536903955577381
2	0.0020837624562432934
3	0.07284563088182676
4	0.0
5	0.0
6	0.0
7	1.1678904622012558E-4
8	2.45871676252896E-5
9	2.274313005339288E-4
10-14	3.9339468200463355E-5
15-19	0.0
20-24	8.777618842228386E-4
25-29	0.005109213432535178
30-34	9.797986298677906E-4
35-39	0.0038970660686084018
40-44	0.019839385556846178
45-49	0.0016706980401384281
50-54	0.005881250495969272
55-59	4.032295490547494E-4
60-64	0.006922517044900287
65-69	0.005099378565485063
70-74	0.003583579681385959
75-79	0.2500773112752018
80-84	0.4526681963573005
85-89	0.3885387163986389
90-94	0.36463138395818856
95-99	0.4495173508261196
100-104	0.6359360264026841
105-109	0.49181219657514275
110-114	0.5132890874958332
115-119	0.47965753025958086
120-124	0.6385459542461086
125-129	0.8583208107815222
130-134	0.6479984908396512
135-139	0.4518728014846224
140-144	0.5827884048638581
145-149	0.5238210007481261
150-152	0.6124007797656298
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
152	1.6268649E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.6343430508436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.57556703556564	96.24360728381845
2	1.23290407245897	2.407475583184824
3	0.09643428034790943	0.2824589282804704
4	0.03576008386639273	0.13965649182953302
5	0.01610300987281403	0.07861033950367231
6	0.013805703762691593	0.08087464903349718
7	0.005934959086313382	0.04056190819980894
8	0.005990432777020303	0.046789757501969426
9	0.003947843698200153	0.03469006133470663
>10	0.013246278627826317	0.1916105519743601
>50	1.946599933531341E-4	0.012067362727605946
>100	7.385642069587062E-5	0.015456008001970667
>500	0.0	0.0
>1k	2.5192135668871196E-5	0.042159203647703876
>5k	6.295693232155272E-6	0.03665926905206972
>10k+	6.29569323215527E-6	0.3473226019093225
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	56505	0.3473244766667472	Illumina Paired End PCR Primer 2 (100% over 31bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2293583812644798E-4	0.0	0.0	0.0
2	2.8889921959715276E-4	0.0	0.0	6.1467919063224E-6
3	6.45413150163852E-4	0.0	0.0	1.22935838126448E-5
4	9.343123697610048E-4	0.0	0.0	1.22935838126448E-5
5	0.0012600923407960919	0.0	6.1467919063224E-6	1.22935838126448E-5
6	0.0015428447684869223	0.0	6.1467919063224E-6	1.22935838126448E-5
7	0.0017764228609271735	0.0	6.1467919063224E-6	1.84403757189672E-5
8	0.002089909248149616	0.0	6.1467919063224E-6	1.84403757189672E-5
9	0.002427982802997348	0.0	1.22935838126448E-5	1.84403757189672E-5
10-14	0.004516662692765699	3.68807514379344E-6	3.1963317912876483E-5	2.089909248149616E-5
15-19	0.030173372109755393	6.146791906322399E-6	4.671561848805023E-5	2.4587167625289595E-5
20-24	0.4178798128842782	6.146791906322399E-6	6.269727744448848E-5	3.196331791287648E-5
25-29	0.5122355273630895	1.1064225431380319E-5	7.990829478219119E-5	3.9339468200463355E-5
30-34	0.8326899178905389	1.2293583812644798E-5	9.711931211989391E-5	5.2862410394372636E-5
35-39	1.792452464860481	1.2293583812644798E-5	1.2908263003277038E-4	6.269727744448847E-5
40-44	3.7923702207847745	1.2293583812644798E-5	1.4998172251426655E-4	8.113765316345567E-5
45-49	7.003128532676561	1.2293583812644798E-5	1.905505490959944E-4	8.605508668851359E-5
50-54	11.307906390997804	1.2293583812644798E-5	2.1267899995875504E-4	9.09725202135715E-5
55-59	16.33977965840925	1.721101733770272E-5	2.2866065891519326E-4	9.343123697610048E-5
60-64	21.91016844730008	2.335780924402512E-5	2.348074508215157E-4	1.0203674564495185E-4
65-69	25.654590003140402	2.4587167625289595E-5	2.7168820225945004E-4	1.389174970828862E-4
70-74	25.775182684192156	2.7045884387818556E-5	3.368441964664675E-4	1.6719273985196928E-4
75-79	25.776568171087842	3.0733959531611995E-5	3.4422034675405435E-4	1.9546798262105232E-4
80-84	25.77687796939992	3.442203467540544E-5	3.724955895231374E-4	2.1513771672128398E-4
85-89	25.776919767584882	3.68807514379344E-5	3.847891733357822E-4	2.2128450862760637E-4
90-94	25.777153345677323	3.811010981919888E-5	4.093763409610719E-4	2.348074508215157E-4
95-99	25.779501420185532	4.548626010678575E-5	4.364222253488904E-4	2.4587167625289596E-4
100-104	25.7810590172546	4.917433525057919E-5	4.708442600242958E-4	2.618533352093342E-4
105-109	25.784780285074685	5.409176877563711E-5	4.966607860308499E-4	2.839817860720949E-4
110-114	25.7976664196271	6.023856068195951E-5	5.482938380439582E-4	2.9381665312221074E-4
115-119	25.831554912765036	6.146791906322399E-5	5.937800981507437E-4	2.962753698847397E-4
120-124	25.892384794828388	6.146791906322399E-5	6.380369998762651E-4	3.036515201723266E-4
125-129	25.990241721977036	6.146791906322399E-5	6.908994102706377E-4	3.1594510398497133E-4
130-134	26.12222563778959	6.76147109695464E-5	7.179452946584563E-4	3.3315612132267405E-4
135-139	26.293074489467443	8.236701154472016E-5	7.437618206650105E-4	3.491377802791123E-4
140	26.409334911583624	8.605508668851359E-5	7.622021963839776E-4	3.503671386603768E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCA	7665	0.0	51.005924	145-146
GTATCAT	10655	0.0	36.24562	145-146
CGGTTCG	5260	0.0	25.041166	70-74
ATCGGAC	9100	0.0	24.246933	70-74
TCGGAAC	9455	0.0	23.537329	70-74
CGCCGTA	6775	0.0	23.508871	140-144
ATCGGAT	7035	0.0	22.895239	70-74
GATCGGT	7505	0.0	22.842894	70-74
GCGGTTA	6100	0.0	22.598307	70-74
AGCGGTA	6710	0.0	21.56676	70-74
TCGGAAT	7110	0.0	21.483046	70-74
GCCGTAT	7575	0.0	21.27778	140-144
GATCGGC	10295	0.0	21.248056	70-74
TCGCCGT	7590	0.0	21.0425	140-144
CGGCATT	9860	0.0	20.644936	75-79
AGCGGTC	10200	0.0	20.042948	70-74
CCGTATC	7875	0.0	20.038857	140-144
GCGGTTG	9580	0.0	19.66337	70-74
GTCGCCG	8620	0.0	19.600018	140-144
GAACCGA	8850	0.0	19.33184	90-94
>>END_MODULE
