FastQCFastQC Report
Sun 14 Aug 2016
C104_H3K4me1_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameC104_H3K4me1_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16268649
Sequences flagged as poor quality0
Sequence length152
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG565050.3473244766667472Illumina Paired End PCR Primer 2 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA76650.051.005924145-146
GTATCAT106550.036.24562145-146
CGGTTCG52600.025.04116670-74
ATCGGAC91000.024.24693370-74
TCGGAAC94550.023.53732970-74
CGCCGTA67750.023.508871140-144
ATCGGAT70350.022.89523970-74
GATCGGT75050.022.84289470-74
GCGGTTA61000.022.59830770-74
AGCGGTA67100.021.5667670-74
TCGGAAT71100.021.48304670-74
GCCGTAT75750.021.27778140-144
GATCGGC102950.021.24805670-74
TCGCCGT75900.021.0425140-144
CGGCATT98600.020.64493675-79
AGCGGTC102000.020.04294870-74
CCGTATC78750.020.038857140-144
GCGGTTG95800.019.6633770-74
GTCGCCG86200.019.600018140-144
GAACCGA88500.019.3318490-94
Produced by FastQC (version 0.11.3)