FastQCFastQC Report
Sun 14 Aug 2016
C101_input.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameC101_input.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37512507
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC51650.017.79738211
AGCGGTT70150.013.06109110
GAGCGGT70550.012.9020189
AGAGCGG76500.011.9768738
CGGTTCA76000.011.95723512
ATGCCGA77600.011.54323625
AAGAGCG81050.011.3045137
AATGCCG80450.011.15266124
TCGGAAG84850.010.8868093
TGCCGAG82800.010.85464326
ATCGGAA92150.09.9755522
CGGAAGA92800.09.9379844
GATCGGA95700.09.4315451
CTCGTAT11450.09.04211828
TCTCGTA11850.08.61021927
GACCGAT13200.08.06732821
GAGACCG35800.07.711807330
GTTCAGC125300.07.63585314
GAATGCC128000.07.548149623
CCGATCT13700.07.44651223
Produced by FastQC (version 0.11.3)