FastQCFastQC Report
Sun 14 Aug 2016
C101_H3K4me1_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameC101_H3K4me1_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16994724
Sequences flagged as poor quality0
Sequence length152
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG482810.28409405177748104Illumina Paired End PCR Primer 2 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA66000.048.05571145-146
GTATCAT99550.031.933718145-146
ATCGGAC103550.024.18907570-74
TCGGAAC100800.024.06703670-74
CGGTTCG48850.024.02390570-74
CGCCGTA56950.022.987448140-144
GATCGGT66450.022.64726470-74
ATCGGAT60950.022.34395470-74
AGCGGTA68850.022.11222670-74
GCGGTTA62000.022.08326370-74
GATCGGC110150.021.5735770-74
AGCGGTC104450.021.00402870-74
TCGGAAT71250.020.79377270-74
GCCGTAT63400.020.50867140-144
TCGCCGT63750.020.419304140-144
CGGCATT87550.019.61730675-79
TCGGAAA108600.019.4755970-74
CCGTATC66000.019.409058140-144
CGGTTCC122250.019.29499270-74
GTCGCCG72800.019.081114140-144
Produced by FastQC (version 0.11.3)