FastQCFastQC Report
Mon 15 Aug 2016
V503_H3K27ac_ChIPSeq_trimmed_19bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV503_H3K27ac_ChIPSeq_trimmed_19bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35164175
Sequences flagged as poor quality0
Sequence length19
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCG1022300.290722020351679Illumina Paired End Adapter 2 (100% over 19bp)
GATCGGAAGAGCGGTTCAG599570.17050591973222748Illumina Paired End Adapter 2 (100% over 19bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA66100.011.8812372
GATCGGA68200.011.5535231
AATGCCG123000.011.3565413
AGCGGTT74950.010.62177210
TCGGAAG77650.010.2646753
GTTCAGC137000.010.2406393
GAATGCC139000.010.23160212
TTCAGCA145850.09.7215374
GCGGTTC82150.09.72153311
GGAATGC149150.09.61109211
GAGCGGT85250.09.4299159
CAGGAAT152800.09.3568959
CGGAAGA85700.09.3533664
AAGAGCG85550.09.3410197
AGGAATG156300.09.13905210
TCAGCAG172350.08.4659155
CGTATGC11750.07.96747731
AGAGCGG104100.07.9408738
GCAGGAA190750.07.7338048
TATGCCG12200.07.72688343
Produced by FastQC (version 0.11.3)